Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
COLO 858 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.6369 1.3908 0.1215 0.1414 0.1423 0.3571 0.1423 0.2278 1.0870 0.4505 0.2890 0.1570 0.1949 0.1923 0.3290 0.0746 0.0775 0.4237 0.3848
COLO 858 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.9259 2.3310 0.1366 0.1927 0.1125 0.4132 0.1701 0.2857 1.5601 0.6536 0.5025 0.1707 0.2494 0.1880 0.3509 0.1099 0.1302 0.3704 0.5640
COLO 858 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.9259 2.7624 0.1416 0.1927 0.1471 0.4785 0.1799 0.3058 1.6234 0.7143 0.5051 0.1695 0.2506 0.2053 0.3731 0.1189 0.1600 0.4785 0.6401
COLO 858 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
COLO 858 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.6329 1.3210 0.1383 0.2037 0.3390 0.2092 0.2833 1.0616 0.4132 0.3906 0.2070 0.2695 0.1776 0.0221 0.0980 0.4608 0.3971
COLO 858 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.8772 2.1008 0.1148 0.1543 0.1163 0.3636 0.1306 0.1957 1.4493 0.5263 0.3584 0.1479 0.1739 0.1667 0.2865 0.0526 0.0926 0.3472 0.5601
COLO 858 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.8696 2.3641 0.1085 0.1433 0.1252 0.4310 0.1468 0.2155 1.6313 0.5495 0.3663 0.1637 0.1529 0.3175 0.1202 0.4049 0.5592
COLO 858 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.6173 1.3423 0.1471 0.1335 0.2833 0.1464 0.1988 0.8929 0.4219 0.3021 0.1462 0.1859 0.1730 0.3175 0.0323 0.0926 0.3718 0.4002
COLO 858 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.5618 1.1507 0.1116 0.1410 0.1255 0.2591 0.1650 0.2128 0.9434 0.3597 0.3012 0.1443 0.1883 0.1808 0.3145 0.0685 0.0806 0.4405 0.3517
COLO 858 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.8547 2.1598 0.1124 0.1458 0.1214 0.3584 0.1372 0.2387 1.3774 0.5525 0.3497 0.1508 0.1733 0.2950 0.0405 0.1030 0.3460 0.5242
COLO 858 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.8929 2.3202 0.1053 0.1513 0.1189 0.4525 0.1524 0.2538 1.5576 0.5714 0.3279 0.1563 0.1721 0.1698 0.3115 0.0602 0.1217 0.4202 0.5526
COLO 858 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.6024 1.3755 0.1126 0.1653 0.2907 0.1852 0.2232 0.9434 0.3906 0.3345 0.1686 0.2016 0.1721 0.4032 0.0284 0.0935 0.4202 0.3792
COLO 858 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.5747 1.1862 0.1202 0.1792 0.1493 0.2469 0.1961 0.2674 0.9434 0.3436 0.3390 0.1808 0.2024 0.1792 0.4032 0.1133 0.0826 0.4630 0.3549
COLO 858 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.6803 1.4793 0.1223 0.1437 0.1314 0.3030 0.1497 0.2183 1.5060 0.4000 0.3268 0.1592 0.1792 0.1764 0.3378 0.0592 0.0800 0.3774 0.4273
COLO 858 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.8065 1.6287 0.1016 0.1377 0.1289 0.4000 0.1758 0.2433 1.4368 0.4444 0.3322 0.1681 0.1835 0.1980 0.3610 0.0461 0.0820 0.4464 0.4684
COLO 858 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.5618 1.0787 0.1092 0.1539 0.1407 0.2387 0.1618 0.2119 1.0672 0.3571 0.3049 0.1637 0.1764 0.1799 0.3300 0.0420 0.0735 0.4651 0.3681
COLO 858 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.5435 1.0834 0.1220 0.1748 0.1431 0.2849 0.1686 0.2421 0.9901 0.3390 0.3125 0.1724 0.1984 0.2016 0.3636 0.0658 0.0909 0.5405 0.3621
COLO 858 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.6711 1.4225 0.1220 0.1513 0.0952 0.3049 0.1383 0.2088 1.3228 0.4149 0.3367 0.1707 0.2933 0.0442 0.0893 0.3096 0.4092
COLO 858 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.7299 1.9724 0.1125 0.1522 0.1067 0.4219 0.1431 0.2252 1.7153 0.4587 0.3704 0.1675 0.1764 0.1745 0.3115 0.0541 0.3891 0.5266
COLO 858 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.5682 1.1198 0.1256 0.1908 0.1449 0.2336 0.2137 0.2532 1.0000 0.3521 0.3497 0.1707 0.2016 0.1873 0.3521 0.0448 0.0763 0.4184 0.3674
COLO 858 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.5650 1.0953 0.1264 0.2088 0.1462 0.2899 0.2193 0.2849 1.0000 0.3367 0.3677 0.2141 0.1916 0.3521 0.0901 0.4975 0.3764
COLO 858 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.5682 1.2048 0.1258 0.1642 0.1482 0.2500 0.1546 0.2463 0.8929 0.3718 0.3145 0.1704 0.1976 0.1684 0.3257 0.0962 0.0685 0.3876 0.3847
COLO 858 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.6061 1.0741 0.1321 0.1751 0.1355 0.2899 0.1724 0.2584 0.7813 0.4082 0.3559 0.1661 0.2033 0.1795 0.3677 0.0676 0.0885 0.4464 0.3937
COLO 858 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.6135 1.1655 0.1437 0.2222 0.1855 0.2392 0.1845 0.2755 0.9524 0.3484 0.3460 0.2105 0.2342 0.2500 0.3953 0.0990 0.0877 0.4975 0.4143
COLO 858 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.6135 1.0695 0.1421 0.2183 0.1812 0.2646 0.1838 0.2967 0.8547 0.3636 0.3472 0.2024 0.2347 0.2770 0.4329 0.0645 0.0787 0.5714 0.4938