Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
COLO 858 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0893 0.3731 0.0049 0.0036 0.0061 0.0077 0.0094 0.0176 0.0676 0.1621 0.0432 0.0031 0.0118 0.0070 0.0299 0.0062 0.0679 0.0082 0.0066 0.1131 0.0250
COLO 858 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0561 0.3534 0.0048 0.0034 0.0063 0.0096 0.0082 0.0147 0.0593 0.2066 0.0277 0.0037 0.0132 0.0076 0.0352 0.0053 0.0556 0.0108 0.0059 0.0953 0.0188
COLO 858 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0513 0.4016 0.0056 0.0045 0.0061 0.0102 0.0089 0.0164 0.0620 0.1969 0.0273 0.0036 0.0153 0.0081 0.0400 0.0062 0.0580 0.0094 0.0060 0.1038 0.0195
COLO 858 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0756 0.3497 0.0045 0.0040 0.0053 0.0061 0.0092 0.0219 0.0565 0.3279 0.0379 0.0035 0.0116 0.0075 0.0150 0.0051 0.0736 0.0061 0.0090 0.1585 0.0189
COLO 858 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0801 0.3891 0.0040 0.0032 0.0047 0.0065 0.0090 0.0184 0.0588 0.3086 0.0332 0.0032 0.0124 0.0070 0.0160 0.0052 0.0814 0.0071 0.2037 0.0191
COLO 858 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0615 0.4926 0.0046 0.0031 0.0059 0.0092 0.0087 0.0153 0.0449 0.3268 0.0164 0.0036 0.0161 0.0082 0.0266 0.0048 0.0546 0.0101 0.0066 0.1471 0.0268
COLO 858 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0635 0.5102 0.0048 0.0043 0.0057 0.0096 0.0094 0.0183 0.0433 0.3333 0.0240 0.0039 0.0164 0.0094 0.0305 0.0047 0.0633 0.0100 0.0066 0.1451 0.0296
COLO 858 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.1103 0.4651 0.0053 0.0053 0.0072 0.0086 0.0126 0.0246 0.0670 0.2725 0.0427 0.0050 0.0134 0.0086 0.0462 0.0074 0.0514 0.0099 0.0058 0.0601 0.0232
COLO 858 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.1148 0.4854 0.0057 0.0050 0.0079 0.0094 0.0143 0.0270 0.0614 0.2577 0.0401 0.0051 0.0131 0.0080 0.0475 0.0058 0.0484 0.0087 0.0056 0.0842 0.0317
COLO 858 0.0000
uM
 48
hr
 8
InnoScan/Mapix
COLO 858 0.0000
uM
 48
hr
 8
InnoScan/Mapix
COLO 858 0.0000
uM
 48
hr
 8
InnoScan/Mapix
COLO 858 0.0000
uM
 48
hr
 8
InnoScan/Mapix
COLO 858 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0786 0.5376 0.0045 0.0038 0.0064 0.0084 0.0100 0.0228 0.0529 0.3460 0.0210 0.0035 0.0189 0.0082 0.0295 0.0070 0.0589 0.0080 0.0066 0.1064 0.0187
COLO 858 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0918 0.5780 0.0052 0.0043 0.0061 0.0099 0.0105 0.0231 0.0520 0.3367 0.0212 0.0040 0.0185 0.0090 0.0324 0.0056 0.0450 0.0080 0.0056 0.1133 0.0221
COLO 858 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.7634 1.9455 0.1642 0.1130 0.3279 0.1715 0.2141 1.2346 0.5155 0.3690 0.1541 0.2008 0.2299 0.3247 0.0909 0.0952 0.3521 0.5328
COLO 858 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 1.0504 2.7701 0.1227 0.1618 0.1157 0.4219 0.1407 0.2294 1.5748 0.7042 0.3937 0.1447 0.1992 0.2532 0.3300 0.0952 0.1311 0.4405 0.6265
COLO 858 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.6289 1.1876 0.1393 0.1520 0.1866 0.2433 0.1736 0.2227 1.1312 0.3745 0.3534 0.1721 0.1883 0.2331 0.4425 0.0415 0.0943 0.4878 0.3735
COLO 858 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.6211 1.1403 0.1605 0.1689 0.1969 0.2506 0.2128 0.2786 0.8475 0.3650 0.3436 0.1992 0.2174 0.2618 0.4255 0.0820 0.0719 0.5435 0.3652
COLO 858 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.7937 2.0492 0.1333 0.2151 0.1284 0.3257 0.1916 0.2907 1.2422 0.5155 0.4608 0.1493 0.2160 0.2342 0.3704 0.0730 0.0990 0.3861 0.5126
COLO 858 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 1.0000 2.8571 0.1466 0.2198 0.1377 0.4149 0.1919 0.3185 1.5552 0.6667 0.4525 0.1342 0.2288 0.2217 0.3876 0.1032 0.1451 0.4651 0.6431
COLO 858 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
COLO 858 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.5988 1.1390 0.1366 0.2012 0.1876 0.2198 0.2016 0.2801 0.7519 0.3663 0.4348 0.2058 0.2646 0.2101 0.4464 0.0503 0.0730 0.4831 0.3763
COLO 858 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.9434 2.6110 0.1155 0.2012 0.1280 0.3876 0.1460 0.2564 1.6949 0.6098 0.5051 0.1592 0.2375 0.2469 0.3610 0.0671 0.1309 0.4367 0.6189
COLO 858 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.9434 2.6385 0.1477 0.1887 0.1203 0.4000 0.1534 0.2849 1.8215 0.6757 0.4717 0.1522 0.2597 0.2740 0.3846 0.1456 0.1284 0.5291 0.6337