Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
COLO 858 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0573 0.6711 0.0064 0.0049 0.0079 0.0076 0.0118 0.0204 0.0648 0.2994 0.0343 0.0053 0.0176 0.0098 0.0307 0.0074 0.0812 0.0127 0.0101 0.1255 0.0234
COLO 858 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0578 0.7042 0.0059 0.0045 0.0091 0.0090 0.0119 0.0198 0.0514 0.2967 0.0302 0.0047 0.0181 0.0096 0.0316 0.0050 0.0904 0.0114 0.0104 0.1730 0.0194
COLO 858 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0491 0.4184 0.0042 0.0043 0.0086 0.0081 0.0098 0.0177 0.0748 0.2632 0.0316 0.0037 0.0130 0.0075 0.0188 0.0059 0.0702 0.0074 0.0203 0.1276 0.0190
COLO 858 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0556 0.3718 0.0046 0.0049 0.0088 0.0089 0.0111 0.0219 0.0746 0.2740 0.0348 0.0035 0.0138 0.0075 0.0221 0.0063 0.0772 0.0098 0.0235 0.1389 0.0186
COLO 858 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0704 0.6289 0.0051 0.0066 0.0058 0.0070 0.0156 0.0306 0.0700 0.4016 0.0051 0.0155 0.0093 0.0261 0.0044 0.0839 0.0074 0.0092 0.1675 0.0285
COLO 858 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0765 0.5882 0.0057 0.0064 0.0065 0.0073 0.0157 0.0365 0.0711 0.3953 0.0430 0.0062 0.0164 0.0086 0.0277 0.0051 0.0937 0.0082 0.0108 0.2160 0.0297
COLO 858 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0594 0.3401 0.0045 0.0048 0.0064 0.0108 0.0108 0.0233 0.0581 0.2933 0.0292 0.0039 0.0162 0.0067 0.0233 0.0067 0.0589 0.0075 0.0061 0.1129 0.0224
COLO 858 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0737 0.4386 0.0050 0.0054 0.0069 0.0126 0.0123 0.0245 0.0637 0.3096 0.0306 0.0039 0.0161 0.0079 0.0242 0.0057 0.0587 0.0095 0.0067 0.1054 0.0257
COLO 858 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0900 0.5952 0.0052 0.0056 0.0101 0.0097 0.0121 0.0281 0.0658 0.2165 0.0415 0.0041 0.0156 0.0099 0.0356 0.0072 0.0622 0.0194 0.0091 0.0962 0.0287
COLO 858 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0752 0.9434 0.0051 0.0045 0.0110 0.0126 0.0127 0.0221 0.2392 0.0436 0.0040 0.0174 0.0087 0.0489 0.0080 0.0798 0.0157 0.0099 0.1471 0.0309
COLO 858 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0497 0.3774 0.0045 0.0033 0.0075 0.0087 0.0092 0.0164 0.0569 0.4219 0.0228 0.0028 0.0116 0.0078 0.0242 0.0053 0.0782 0.0099 0.0083 0.1309 0.0163
COLO 858 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0544 0.5181 0.0040 0.0031 0.0084 0.0100 0.0097 0.0149 0.0529 0.4202 0.0258 0.0029 0.0127 0.0087 0.0332 0.0057 0.0888 0.0102 0.0083 0.1748 0.0149
COLO 858 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0791 0.5682 0.0054 0.0041 0.0055 0.0066 0.0105 0.0215 0.0720 0.2849 0.0520 0.0038 0.0155 0.0105 0.0239 0.0047 0.0794 0.0082 0.0103 0.1208 0.0157
COLO 858 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0789 1.0101 0.0052 0.0034 0.0067 0.0078 0.0117 0.0189 0.0806 0.3030 0.0579 0.0038 0.0163 0.0101 0.0341 0.0063 0.0895 0.0085 0.0107 0.1792 0.0280
COLO 858 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0493 0.5128 0.0039 0.0032 0.0068 0.0104 0.0089 0.0159 0.0490 0.3597 0.0234 0.0028 0.0166 0.0093 0.0272 0.0045 0.0703 0.0094 0.0093 0.1385 0.0131
COLO 858 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0505 0.6369 0.0047 0.0035 0.0076 0.0114 0.0102 0.0163 0.0592 0.3436 0.0266 0.0029 0.0189 0.0104 0.0366 0.0057 0.0720 0.0118 0.0070 0.1587 0.0147
COLO 858 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0692 0.4831 0.0054 0.0046 0.0072 0.0079 0.0097 0.0214 0.0678 0.2778 0.0386 0.0039 0.0126 0.0086 0.0377 0.0781 0.0119 0.0086 0.1171 0.0410
COLO 858 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0708 0.5128 0.0048 0.0032 0.0073 0.0090 0.0095 0.0171 0.0708 0.2481 0.0366 0.0037 0.0127 0.0079 0.0521 0.0055 0.0953 0.0107 0.0090 0.1645 0.0375
COLO 858 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0518 0.4065 0.0047 0.0031 0.0067 0.0080 0.0086 0.0146 0.0568 0.2632 0.0263 0.0026 0.0128 0.0077 0.0365 0.0056 0.0793 0.0073 0.0074 0.1195 0.0182
COLO 858 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0547 0.4386 0.0052 0.0033 0.0062 0.0090 0.0091 0.0145 0.0569 0.2475 0.0283 0.0027 0.0146 0.0089 0.0422 0.0056 0.0812 0.0094 0.0065 0.1328 0.0185
COLO 858 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0675 0.6250 0.0051 0.0041 0.0062 0.0069 0.0096 0.0227 0.0647 0.4367 0.0436 0.0038 0.0153 0.0102 0.0282 0.0055 0.0914 0.0077 0.0107 0.2101 0.0317
COLO 858 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0730 0.6803 0.0053 0.0034 0.0069 0.0086 0.0191 0.0659 0.4132 0.0475 0.0038 0.0164 0.0100 0.0384 0.0058 0.1003 0.0091 0.0113 0.2688 0.0359
COLO 858 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0485 0.4975 0.0039 0.0028 0.0070 0.0107 0.0095 0.0143 0.0572 0.3623 0.0184 0.0040 0.0160 0.0089 0.0283 0.0811 0.0108 0.0079 0.1484 0.0192
COLO 858 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0501 0.4566 0.0043 0.0037 0.0068 0.0123 0.0093 0.0139 0.0600 0.3774 0.0192 0.0038 0.0169 0.0097 0.0394 0.0055 0.0858 0.0115 0.0080 0.1603 0.0167
COLO 858 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0820 0.3584 0.0061 0.0047 0.0066 0.0070 0.0098 0.0220 0.0687 0.1792 0.0405 0.0038 0.0119 0.0073 0.0274 0.0055 0.0593 0.0078 0.0063 0.0830 0.0264