Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
COLO 858 0.0000
uM
 10
hr
 1
InnoScan/Mapix
COLO 858 0.0000
uM
 10
hr
 1
InnoScan/Mapix
COLO 858 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0365 0.4630 0.0032 0.0030 0.0060 0.0053 0.0065 0.0129 0.0634 0.4348 0.0987 0.0026 0.0169 0.0081 0.0134 0.0047 0.1098 0.0100 0.0103 0.1003 0.0083
COLO 858 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0404 0.0035 0.0034 0.0064 0.0062 0.0074 0.0125 0.0704 0.4525 0.1030 0.0028 0.0192 0.0077 0.0209 0.0059 0.1140 0.0109 0.0107 0.1253 0.0141
COLO 858 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0586 0.5319 0.0039 0.0036 0.0075 0.0067 0.0087 0.0171 0.0649 0.2717 0.1441 0.0029 0.0223 0.0082 0.0199 0.0076 0.0441 0.0098 0.0050 0.0432 0.0279
COLO 858 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0609 0.5181 0.0046 0.0046 0.0075 0.0081 0.0106 0.0190 0.0749 0.2717 0.1623 0.0037 0.0245 0.0235 0.0091 0.0415 0.0130 0.0048 0.0498 0.0366
COLO 858 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0433 0.3356 0.0041 0.0041 0.0120 0.0096 0.0085 0.0189 0.0491 0.3623 0.1114 0.0036 0.0161 0.0092 0.0135 0.0075 0.0830 0.0221 0.0076 0.0687 0.0173
COLO 858 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0479 0.3597 0.0040 0.0037 0.0130 0.0104 0.0095 0.0180 0.0561 0.3195 0.1131 0.0039 0.0170 0.0071 0.0180 0.0049 0.0897 0.0204 0.0080 0.0834 0.0189
COLO 858 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0412 0.3745 0.0033 0.0032 0.0063 0.0055 0.0068 0.0117 0.0619 0.2985 0.1034 0.0025 0.0193 0.0074 0.0162 0.0049 0.0548 0.0087 0.0051 0.0555 0.0126
COLO 858 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0394 0.3774 0.0034 0.0031 0.0058 0.0066 0.0079 0.0132 0.0595 0.2817 0.1130 0.0030 0.0199 0.0076 0.0209 0.0059 0.0541 0.0095 0.0044 0.0620 0.0124
COLO 858 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0327 0.4016 0.0031 0.0027 0.0065 0.0059 0.0064 0.0116 0.0569 0.3125 0.0862 0.0023 0.0150 0.0066 0.0119 0.0049 0.0887 0.0104 0.0072 0.0735 0.0123
COLO 858 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0344 0.4255 0.0032 0.0029 0.0069 0.0070 0.0071 0.0120 0.0617 0.2793 0.0876 0.0028 0.0166 0.0063 0.0134 0.0055 0.0915 0.0135 0.0054 0.0907 0.0137
COLO 858 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0483 0.4546 0.0041 0.0026 0.0075 0.0068 0.0061 0.0111 0.0641 0.2778 0.0990 0.0025 0.0189 0.0067 0.0124 0.0061 0.0522 0.0081 0.0052 0.0562 0.0261
COLO 858 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0531 0.4329 0.0045 0.0035 0.0081 0.0084 0.0081 0.0157 0.0639 0.3215 0.1114 0.0033 0.0202 0.0081 0.0177 0.0076 0.0527 0.0115 0.0062 0.0628 0.0276
COLO 858 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0455 0.3677 0.0040 0.0049 0.0104 0.0091 0.0199 0.0461 0.3759 0.1023 0.0037 0.0157 0.0071 0.0139 0.0072 0.0781 0.0243 0.0077 0.0760 0.0276
COLO 858 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0481 0.3817 0.0040 0.0135 0.0118 0.0106 0.0197 0.0557 0.3484 0.0953 0.0039 0.0163 0.0070 0.0141 0.0049 0.0875 0.0237 0.0085 0.0962 0.0211
COLO 858 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0476 0.2740 0.0039 0.0029 0.0080 0.0065 0.0076 0.0135 0.0661 0.2841 0.1261 0.0032 0.0137 0.0060 0.0145 0.0056 0.0588 0.0120 0.0052 0.0584 0.0143
COLO 858 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0458 0.2849 0.0039 0.0034 0.0094 0.0072 0.0091 0.0149 0.0673 0.2577 0.1337 0.0033 0.0147 0.0060 0.0166 0.0058 0.0583 0.0127 0.0055 0.0634 0.0143
COLO 858 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0368 0.3861 0.0033 0.0028 0.0083 0.0066 0.0075 0.0134 0.0700 0.3086 0.0810 0.0027 0.0157 0.0061 0.0116 0.0054 0.0959 0.0113 0.0083 0.0765 0.0148
COLO 858 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0371 0.4115 0.0034 0.0032 0.0065 0.0079 0.0083 0.0137 0.0635 0.2849 0.0874 0.0030 0.0161 0.0060 0.0128 0.0066 0.1003 0.0128 0.0098 0.0887 0.0131
COLO 858 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0596 0.4854 0.0037 0.0026 0.0051 0.0050 0.0060 0.0105 0.0694 0.2703 0.1115 0.0024 0.0205 0.0076 0.0153 0.0064 0.0393 0.0061 0.0046 0.0457 0.0188
COLO 858 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0585 0.4673 0.0071 0.0034 0.0054 0.0057 0.0081 0.0142 0.0684 0.2538 0.1120 0.0031 0.0217 0.0079 0.0167 0.0075 0.0386 0.0076 0.0053 0.0469 0.0187
COLO 858 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0412 0.3401 0.0039 0.0036 0.0093 0.0074 0.0081 0.0148 0.0572 0.3401 0.0940 0.0029 0.0156 0.0070 0.0130 0.0069 0.0820 0.0202 0.0077 0.0585 0.0208
COLO 858 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0439 0.3300 0.0039 0.0033 0.0110 0.0083 0.0095 0.0153 0.0470 0.3300 0.0955 0.0030 0.0162 0.0065 0.0124 0.0050 0.0871 0.0151 0.0077 0.0726 0.0165
COLO 858 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0597 0.3559 0.0041 0.0027 0.0068 0.0053 0.0072 0.0111 0.0640 0.3345 0.1229 0.0024 0.0198 0.0074 0.0130 0.0056 0.0629 0.0084 0.0130 0.0680 0.0270