Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
COLO 858 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0283 0.6757 0.0032 0.0033 0.0111 0.0071 0.0150 0.0475 0.6757 0.0336 0.0034 0.0204 0.0086 0.0399 0.0046 0.0768 0.0166 0.0105 0.1374 0.0153
COLO 858 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0433 0.3279 0.0037 0.0028 0.0080 0.0087 0.0084 0.0187 0.0783 0.4566 0.0444 0.0034 0.0131 0.0068 0.0226 0.0065 0.0595 0.0086 0.0064 0.0772 0.0239
COLO 858 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0429 0.3521 0.0039 0.0037 0.0072 0.0098 0.0073 0.0219 0.0659 0.4386 0.0454 0.0037 0.0201 0.0064 0.0230 0.0062 0.0564 0.0096 0.0050 0.0806 0.0247
COLO 858 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0240 0.4132 0.0030 0.0029 0.0079 0.0098 0.0064 0.0123 0.0599 0.5682 0.0393 0.0028 0.0149 0.0068 0.0160 0.0047 0.0653 0.0122 0.0086 0.0980 0.0124
COLO 858 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0258 0.4505 0.0033 0.0030 0.0082 0.0100 0.0069 0.0121 0.0579 0.5181 0.0391 0.0031 0.0151 0.0070 0.0181 0.0041 0.0675 0.0117 0.0061 0.1117 0.0130
COLO 858 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0382 0.4149 0.0043 0.0030 0.0075 0.0094 0.0077 0.0132 0.0587 0.3623 0.0385 0.0026 0.0169 0.0072 0.0184 0.0055 0.0301 0.0087 0.0056 0.0800 0.0290
COLO 858 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0498 0.4505 0.0043 0.0039 0.0067 0.0109 0.0091 0.0167 0.0611 0.3831 0.0384 0.0035 0.0183 0.0075 0.0253 0.0058 0.0356 0.0099 0.0049 0.0843 0.0280
COLO 858 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0333 0.8696 0.0040 0.0035 0.0121 0.0126 0.0079 0.0170 0.0741 0.6944 0.0377 0.0031 0.0288 0.0372 0.0080 0.0851 0.0176 0.0139 0.1028 0.0221
COLO 858 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0376 0.9259 0.0039 0.0033 0.0122 0.0139 0.0091 0.0160 0.0712 0.6452 0.0322 0.0034 0.0288 0.0104 0.0383 0.0047 0.0787 0.0155 0.0129 0.1348 0.0168
COLO 858 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0410 0.3185 0.0041 0.0037 0.0074 0.0082 0.0093 0.0157 0.0696 0.4464 0.0394 0.0036 0.0165 0.0061 0.0166 0.0051 0.0556 0.0082 0.0072 0.0749 0.0211
COLO 858 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0386 0.4587 0.0042 0.0041 0.0072 0.0096 0.0101 0.0225 0.0599 0.4329 0.0355 0.0037 0.0172 0.0061 0.0182 0.0057 0.0585 0.0097 0.0076 0.0861 0.0203
COLO 858 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0279 0.4367 0.0038 0.0027 0.0062 0.0080 0.0068 0.0122 0.0591 0.5495 0.0341 0.0027 0.0155 0.0068 0.0180 0.0043 0.0660 0.0084 0.0092 0.0810 0.0129
COLO 858 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0290 0.4566 0.0042 0.0030 0.0055 0.0085 0.0072 0.0128 0.0569 0.5236 0.0328 0.0028 0.0157 0.0064 0.0179 0.0047 0.0677 0.0088 0.0099 0.1076 0.0121
COLO 858 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0505 0.4505 0.0055 0.0032 0.0080 0.0088 0.0066 0.0119 0.0647 0.3521 0.0429 0.0027 0.0171 0.0069 0.0206 0.0063 0.0317 0.0090 0.0048 0.0545 0.0154
COLO 858 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0533 0.4854 0.0039 0.0076 0.0098 0.0082 0.0146 0.0658 0.3509 0.0432 0.0190 0.0075 0.0212 0.0077 0.0308 0.0079 0.0044 0.0593
COLO 858 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0290 0.6329 0.0035 0.0029 0.0078 0.0070 0.0060 0.0142 0.0664 0.5848 0.0442 0.0025 0.0185 0.0080 0.0306 0.0068 0.0683 0.0118 0.0092 0.0760 0.0191
COLO 858 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0310 0.6329 0.0032 0.0030 0.0080 0.0080 0.0063 0.0139 0.0512 0.5917 0.0393 0.0027 0.0187 0.0077 0.0318 0.0049 0.0696 0.0081 0.0100 0.0129
COLO 858 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0587 0.3610 0.0040 0.0035 0.0078 0.0081 0.0080 0.0164 0.0728 0.3968 0.0476 0.0031 0.0171 0.0064 0.0198 0.0049 0.0417 0.0103 0.0132 0.0603 0.0244
COLO 858 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0533 0.3559 0.0043 0.0040 0.0072 0.0078 0.0087 0.0172 0.0690 0.4115 0.0488 0.0036 0.0161 0.0063 0.0190 0.0056 0.0441 0.0091 0.0153 0.0672
COLO 858 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0385 0.3704 0.0039 0.0037 0.0059 0.0072 0.0095 0.0170 0.0772 0.4032 0.0400 0.0044 0.0189 0.0066 0.0134 0.0045 0.0330 0.0108 0.0049 0.0576 0.0181
COLO 858 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0371 0.4202 0.0038 0.0043 0.0063 0.0074 0.0094 0.0153 0.3759 0.0341 0.0037 0.0162 0.0064 0.0170 0.0041 0.0333 0.0074 0.0052 0.0683 0.0219
COLO 858 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0597 0.5155 0.0045 0.0050 0.0074 0.0118 0.0100 0.0180 0.0560 0.4082 0.0385 0.0031 0.0231 0.0096 0.0196 0.0062 0.0285 0.0107 0.0044 0.0554 0.0217
COLO 858 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0527 0.4975 0.0048 0.0044 0.0068 0.0124 0.0116 0.0205 0.0582 0.3922 0.0349 0.0039 0.0220 0.0090 0.0242 0.0061 0.0298 0.0098 0.0042 0.0607 0.0164
COLO 858 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0545 0.0046 0.0057 0.0206 0.0117 0.0126 0.0285 0.0626 0.2463 0.1385 0.0046 0.0154 0.0074 0.0135 0.0080 0.0634 0.0336 0.0057 0.0435 0.0276
COLO 858 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0578 0.4525 0.0059 0.0067 0.0223 0.0112 0.0139 0.0288 0.0797 0.2278 0.1372 0.0044 0.0168 0.0064 0.0210 0.0086 0.0614 0.0386 0.0056 0.0523 0.0285