Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
COLO 858 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0288 0.7194 0.0035 0.0034 0.0099 0.0123 0.0070 0.0142 0.0651 0.6135 0.0321 0.0030 0.0239 0.0089 0.0326 0.0079 0.0696 0.0149 0.0106 0.0994 0.0152
COLO 858 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0309 0.7353 0.0038 0.0033 0.0116 0.0133 0.0084 0.0146 0.0627 0.5714 0.0295 0.0035 0.0237 0.0093 0.0349 0.0057 0.0742 0.0136 0.0118 0.1218 0.0138
COLO 858 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0402 0.4951 0.0049 0.0039 0.0071 0.0085 0.0101 0.0171 0.0655 0.5988 0.0424 0.0052 0.0192 0.0089 0.0310 0.0053 0.0719 0.0090 0.0087 0.1109 0.0176
COLO 858 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0388 0.4785 0.0043 0.0044 0.0082 0.0090 0.0113 0.0196 0.0707 0.5650 0.0395 0.0041 0.0198 0.0085 0.0340 0.0061 0.0758 0.0110 0.0100 0.1247 0.0179
COLO 858 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0267 0.3425 0.0037 0.0025 0.0060 0.0074 0.0061 0.0132 0.0535 0.3861 0.0341 0.0027 0.0152 0.0060 0.0162 0.0060 0.0373 0.0072 0.0052 0.0590 0.0115
COLO 858 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0293 0.3677 0.0039 0.0028 0.0053 0.0079 0.0073 0.0117 0.0523 0.3623 0.0385 0.0030 0.0168 0.0064 0.0168 0.0046 0.0347 0.0119 0.0059 0.0676 0.0111
COLO 858 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0433 0.5495 0.0041 0.0029 0.0067 0.0086 0.0076 0.0138 0.0657 0.4405 0.0354 0.0027 0.0164 0.0066 0.0198 0.0051 0.0265 0.0069 0.0050 0.0547 0.0146
COLO 858 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0501 0.5236 0.0049 0.0039 0.0070 0.0110 0.0096 0.0178 0.0659 0.4292 0.0381 0.0037 0.0191 0.0053 0.0217 0.0066 0.0280 0.0083 0.0048 0.0680 0.0145
COLO 858 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0304 0.8000 0.0037 0.0031 0.0095 0.0105 0.0075 0.0144 0.0655 0.6993 0.0365 0.0029 0.0255 0.0099 0.0400 0.0078 0.0754 0.0208 0.0121 0.1006 0.0215
COLO 858 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0310 0.8333 0.0038 0.0035 0.0115 0.0120 0.0086 0.0140 0.0517 0.6757 0.0326 0.0031 0.0258 0.0091 0.0393 0.0048 0.0820 0.0151 0.0125 0.0147
COLO 858 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0540 0.4878 0.0053 0.0048 0.0132 0.0096 0.0134 0.0239 0.0919 0.4831 0.0592 0.0049 0.0194 0.0083 0.0266 0.0084 0.0589 0.0120 0.0163 0.0821 0.0194
COLO 858 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0487 0.5051 0.0052 0.0059 0.0143 0.0110 0.0148 0.0280 0.0891 0.5076 0.0060 0.0202 0.0081 0.0269 0.0065 0.0674 0.0136 0.0177 0.0944 0.0272
COLO 858 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0282 0.4000 0.0048 0.0026 0.0068 0.0082 0.0072 0.0127 0.0438 0.4255 0.0425 0.0026 0.0185 0.0080 0.0152 0.0034 0.0429 0.0138 0.0054 0.0677 0.0139
COLO 858 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0290 0.3521 0.0039 0.0031 0.0079 0.0090 0.0073 0.0124 0.0460 0.4115 0.0397 0.0031 0.0180 0.0071 0.0166 0.0043 0.0499 0.0112 0.0061 0.0805 0.0168
COLO 858 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0549 0.5682 0.0049 0.0032 0.0068 0.0095 0.0077 0.0159 0.0577 0.3788 0.0383 0.0030 0.0270 0.0097 0.0210 0.0071 0.0342 0.0062 0.0050 0.0858 0.0265
COLO 858 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0541 0.5208 0.0051 0.0043 0.0064 0.0116 0.0089 0.0196 0.0638 0.3891 0.0386 0.0041 0.0237 0.0113 0.0230 0.0076 0.0319 0.0087 0.0054 0.0829 0.0231
COLO 858 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0333 0.6452 0.0044 0.0045 0.0136 0.0140 0.0102 0.0199 0.0481 0.5988 0.0363 0.0038 0.0202 0.0348 0.0074 0.0681 0.0132 0.0858 0.0175
COLO 858 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0306 0.0034 0.0051 0.0131 0.0154 0.0092 0.0189 0.0679 0.5263 0.0370 0.0031 0.0200 0.0083 0.0092 0.0773 0.0197 0.0107 0.1127 0.0173
COLO 858 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0282 0.4016 0.0040 0.0033 0.0092 0.0090 0.0066 0.0165 0.0625 0.4405 0.0424 0.0026 0.0158 0.0066 0.0220 0.0053 0.0538 0.0105 0.0060 0.0638 0.0116
COLO 858 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0334 0.4673 0.0038 0.0038 0.0103 0.0106 0.0075 0.0196 0.0642 0.4115 0.0402 0.0028 0.0157 0.0069 0.0262 0.0051 0.0534 0.0120 0.0052 0.0753 0.0145
COLO 858 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0253 0.5435 0.0029 0.0043 0.0081 0.0095 0.0073 0.0158 0.0606 0.5650 0.0422 0.0031 0.0181 0.0077 0.0167 0.0042 0.0666 0.0125 0.0089 0.0898 0.0139
COLO 858 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0288 0.6329 0.0034 0.0039 0.0077 0.0108 0.0083 0.0155 0.0544 0.5405 0.0456 0.0034 0.0187 0.0075 0.0288 0.0043 0.0652 0.0118 0.0085 0.1140 0.0118
COLO 858 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0415 0.5495 0.0043 0.0033 0.0081 0.0100 0.0077 0.0165 0.0565 0.3922 0.0422 0.0027 0.0183 0.0079 0.0229 0.0049 0.0322 0.0086 0.0050 0.0525 0.0278
COLO 858 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0489 0.5618 0.0044 0.0037 0.0089 0.0119 0.0111 0.0177 0.0600 0.4098 0.0440 0.0031 0.0204 0.0081 0.0276 0.0056 0.0299 0.0105 0.0043 0.0585
COLO 858 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0294 0.6452 0.0037 0.0036 0.0108 0.0109 0.0069 0.0150 0.0473 0.7463 0.0297 0.0029 0.0205 0.0092 0.0324 0.0043 0.0689 0.0180 0.0103 0.1049 0.0151