Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
COLO 858 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0554 0.4673 0.0046 0.0035 0.0070 0.0093 0.0094 0.0151 0.0664 0.2433 0.0560 0.0038 0.0224 0.0076 0.0242 0.0079 0.0249 0.0100 0.0045 0.0459 0.0226
COLO 858 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0528 0.4484 0.0052 0.0036 0.0081 0.0107 0.0104 0.0186 0.0610 0.2558 0.0497 0.0041 0.0236 0.0094 0.0232 0.0072 0.0228 0.0092 0.0046 0.0469 0.0125
COLO 858 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0277 0.2359 0.0035 0.0031 0.0085 0.0066 0.0075 0.0113 0.0575 0.3257 0.0686 0.0025 0.0123 0.0119 0.0120 0.0075 0.0465 0.0079 0.0781 0.0126
COLO 858 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0269 0.2950 0.0031 0.0031 0.0095 0.0090 0.0064 0.0115 0.0607 0.2674 0.0696 0.0024 0.0133 0.0059 0.0174 0.0075 0.0476 0.0123 0.0078 0.0949 0.0125
COLO 858 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0430 0.4000 0.0040 0.0036 0.0072 0.0092 0.0078 0.0146 0.0523 0.2494 0.0355 0.0030 0.0202 0.0086 0.0251 0.0068 0.0300 0.0098 0.0048 0.0643 0.0102
COLO 858 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0476 0.4630 0.0037 0.0035 0.0075 0.0095 0.0095 0.0142 0.0536 0.2232 0.0416 0.0032 0.0214 0.0094 0.0253 0.0059 0.0279 0.0116 0.0042 0.0700 0.0125
COLO 858 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0283 0.2315 0.0028 0.0061 0.0065 0.0053 0.0082 0.0784 0.4329 0.0663 0.0026 0.0125 0.0065 0.0111 0.0053 0.0715 0.0081 0.0118 0.0942 0.0112
COLO 858 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0289 0.3247 0.0029 0.0029 0.0060 0.0073 0.0065 0.0080 0.0846 0.4255 0.0874 0.0029 0.0132 0.0061 0.0172 0.0056 0.0659 0.0084 0.0113 0.1097 0.0130
COLO 858 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0474 0.4484 0.0041 0.0032 0.0078 0.0089 0.0093 0.0157 0.0661 0.2695 0.0469 0.0030 0.0227 0.0081 0.0247 0.0057 0.0235 0.0100 0.0046 0.0422 0.0120
COLO 858 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0505 0.4854 0.0045 0.0041 0.0084 0.0113 0.0102 0.0167 0.0638 0.2849 0.0538 0.0035 0.0242 0.0083 0.0303 0.0077 0.0226 0.0106 0.0041 0.0451 0.0125
COLO 858 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0330 0.3345 0.0031 0.0035 0.0057 0.0059 0.0092 0.0123 0.0794 0.3165 0.0659 0.0032 0.0187 0.0081 0.0161 0.0082 0.0466 0.0087 0.0068 0.0712 0.0159
COLO 858 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0348 0.3937 0.0029 0.0042 0.0063 0.0070 0.0104 0.0142 0.0809 0.2809 0.0806 0.0035 0.0201 0.0076 0.0181 0.0064 0.0450 0.0080 0.0076 0.0852 0.0190
COLO 858 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0489 0.2933 0.0040 0.0029 0.0076 0.0074 0.0097 0.0147 0.0672 0.2188 0.0455 0.0028 0.0152 0.0067 0.0151 0.0083 0.0295 0.0087 0.0050 0.0590 0.0104
COLO 858 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0475 0.2933 0.0041 0.0034 0.0064 0.0085 0.0091 0.0137 0.0612 0.2114 0.0484 0.0031 0.0164 0.0064 0.0188 0.0061 0.0298 0.0100 0.0042 0.0645 0.0121
COLO 858 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0326 0.2625 0.0034 0.0033 0.0069 0.0072 0.0072 0.0114 0.0976 0.2653 0.0728 0.0032 0.0158 0.0077 0.0140 0.0073 0.0350 0.0101 0.0054 0.0552 0.0201
COLO 858 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0339 0.2857 0.0055 0.0034 0.0073 0.0081 0.0081 0.0113 0.0732 0.2392 0.0709 0.0036 0.0166 0.0067 0.0166 0.0059 0.0354 0.0085 0.0044 0.0684 0.0213
COLO 858 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0494 0.3937 0.0043 0.0029 0.0072 0.0093 0.0091 0.0133 0.0606 0.2857 0.0416 0.0031 0.0162 0.0071 0.0138 0.0059 0.0291 0.0117 0.0052 0.0497 0.0117
COLO 858 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0517 0.3311 0.0042 0.0037 0.0066 0.0109 0.0108 0.0172 0.0612 0.3003 0.0453 0.0035 0.0203 0.0068 0.0070 0.0305 0.0106 0.0045 0.0620 0.0128
COLO 858 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0392 0.2865 0.0046 0.0036 0.0061 0.0059 0.0071 0.0124 0.0700 0.2315 0.0604 0.0028 0.0162 0.0076 0.0146 0.0078 0.0271 0.0072 0.0054 0.0458 0.0227
COLO 858 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0478 0.3077 0.0042 0.0026 0.0062 0.0065 0.0075 0.0110 0.0680 0.2141 0.0631 0.0029 0.0160 0.0072 0.0152 0.0076 0.0289 0.0067 0.0057 0.0606 0.0224
COLO 858 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0538 0.4000 0.0042 0.0029 0.0066 0.0085 0.0088 0.0141 0.0667 0.2278 0.0395 0.0031 0.0217 0.0074 0.0206 0.0082 0.0279 0.0100 0.0044 0.0503 0.0137
COLO 858 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0482 0.4202 0.0046 0.0029 0.0069 0.0088 0.0099 0.0156 0.0605 0.2331 0.0411 0.0032 0.0230 0.0074 0.0226 0.0063 0.0219 0.0103 0.0047 0.0517 0.0108
COLO 858 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0385 0.3226 0.0050 0.0028 0.0053 0.0066 0.0101 0.0107 0.0684 0.2703 0.0587 0.0029 0.0179 0.0073 0.0165 0.0057 0.0373 0.0066 0.0060 0.0544 0.0257
COLO 858 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0384 0.3322 0.0042 0.0030 0.0050 0.0068 0.0078 0.0107 0.0573 0.2597 0.0576 0.0029 0.0187 0.0178 0.0057 0.0380 0.0089 0.0074 0.0686 0.0266
COLO 858 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0547 0.4587 0.0043 0.0030 0.0072 0.0099 0.0090 0.0141 0.0689 0.3268 0.0423 0.0040 0.0235 0.0071 0.0250 0.0082 0.0275 0.0097 0.0054 0.0479 0.0221