Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
AZ-628 MMAC-SF 1.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.6897 -0.4723 0.9524 -1.2821 0.4444 -0.2981 0.2008 0.0199 0.4405 0.4323 0.5814 0.1083 1.0000 0.2316 0.5495 0.2021 1.1521 0.0135 0.9091 0.0138 1.3774 0.3330 0.5952 -0.1819 0.4902 0.0152 0.4484 -0.6092 0.1905 -0.0948 0.6211 -0.2528 0.4717 0.1581 0.6329 0.6061 1.0373 0.1420 1.0493 0.4492
AZ-628 MMAC-SF 1.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.7813 -0.4723 1.0764 -1.2821 0.4219 -0.2981 0.2347 0.0199 0.3817 0.4323 0.6849 0.1083 0.9804 0.2316 0.6667 0.2021 1.2500 0.0135 0.9615 0.0138 1.5129 0.3330 0.6098 -0.1819 0.5155 0.0152 0.4739 -0.6092 0.1818 -0.0948 0.6369 -0.2528 0.4762 0.1581 0.6849 0.6061 1.0953 0.1420 1.2005 0.4492
AZ-628 MMAC-SF 1.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.8929 -0.4723 1.1962 -1.2821 -0.2981 0.2519 0.0199 0.5319 0.4323 0.6494 0.1083 1.1765 0.2316 0.6944 0.2021 1.3316 0.0135 1.0905 0.0138 1.4388 0.3330 0.7092 -0.1819 0.6098 0.0152 0.5780 -0.6092 0.2198 -0.0948 0.5747 -0.2528 0.4587 0.1581 0.8130 0.6061 1.1962 0.1420 0.4492
AZ-628 MMAC-SF 1.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.8197 -0.5648 1.4045 -1.1312 0.4878 -0.2657 0.2747 0.0431 0.4695 0.3503 0.8547 0.3864 1.0823 0.2032 0.6757 0.0460 -0.3789 1.1779 0.2001 1.5773 0.3561 0.6024 -0.3058 0.6024 -0.1354 0.4762 -0.6057 0.1988 -0.0056 0.5952 -0.2528 0.5181 0.2031 0.8130 0.6817 1.0331 -0.0240 1.2579 0.4887
AZ-628 MMAC-SF 1.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.7576 -0.5648 1.0893 -1.1312 0.4484 -0.2657 0.2247 0.0431 0.4032 0.3503 0.7299 0.3864 0.9259 0.2032 0.4975 0.0460 0.8929 -0.3789 1.0858 0.2001 1.4706 0.3561 0.5465 -0.3058 0.4167 -0.1354 0.5025 -0.6057 0.2188 -0.0056 -0.2528 0.2031 0.6711 0.6817 0.9901 -0.0240 1.0331 0.4887
AZ-628 MMAC-SF 1.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.8130 -0.5648 1.3089 -1.1312 0.4878 -0.2657 0.2114 0.0431 0.4167 0.3503 0.8065 0.3864 0.9804 0.2032 0.6410 0.0460 0.9901 -0.3789 1.1062 0.2001 1.5848 0.3561 0.6173 -0.3058 0.4673 -0.1354 0.5435 -0.6057 0.2033 -0.0056 0.5917 -0.2528 0.4951 0.2031 0.7407 0.6817 1.0989 -0.0240 1.3298 0.4887
AZ-628 MMAC-SF 1.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.9009 -0.5648 1.2970 -1.1312 -0.2657 0.2618 0.0431 0.4651 0.3503 0.7937 0.3864 1.1765 0.2032 0.6289 0.0460 1.0132 -0.3789 1.0905 0.2001 1.4306 0.3561 0.6289 -0.3058 0.5780 -0.1354 0.5618 -0.6057 0.1953 -0.0056 0.5495 -0.2528 0.4878 0.2031 0.7874 0.6817 1.0121 -0.0240 1.2531 0.4887
AZ-628 MMAC-SF 1.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.5917 -0.5648 1.1013 -1.1312 0.4202 -0.2657 0.2370 0.0431 0.4032 0.3503 0.7519 0.3864 0.9434 0.2032 0.5952 0.0460 0.9524 -0.3789 1.0661 0.2001 1.3550 0.3561 0.4854 -0.3058 0.4505 -0.1354 0.4219 -0.6057 0.1815 -0.0056 0.5952 -0.2528 0.4854 0.2031 0.6329 0.6817 0.9615 -0.0240 1.0246 0.4887
AZ-628 MMAC-SF 1.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.5405 -0.5648 0.9901 -1.1312 0.4546 -0.2657 0.2252 0.0431 0.3448 0.3503 0.6579 0.3864 0.9709 0.2032 0.4762 0.0460 0.8621 -0.3789 1.0941 0.2001 1.3661 0.3561 0.5051 -0.3058 0.3937 -0.1354 0.4049 -0.6057 0.2137 -0.0056 0.6250 -0.2528 0.5000 0.2031 0.5556 0.6817 0.9804 -0.0240 1.0215 0.4887
AZ-628 MMAC-SF 1.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.6410 -0.5648 1.1001 -1.1312 0.4464 -0.2657 0.1980 0.0431 0.3922 0.3503 0.7813 0.3864 0.9009 0.2032 0.5780 0.0460 0.8929 -0.3789 1.1261 0.2001 1.5773 0.3561 0.4762 -0.3058 0.4762 -0.1354 0.4762 -0.6057 0.2227 -0.0056 0.6250 -0.2528 0.4831 0.2031 0.6579 0.6817 0.9524 -0.0240 1.0965 0.4887
AZ-628 MMAC-SF 1.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.7143 -0.5648 1.3106 -1.1312 0.5155 -0.2657 0.2506 0.0431 0.5208 0.3503 0.8197 0.3864 1.0965 0.2032 0.5714 0.0460 0.9524 -0.3789 0.2001 1.4535 0.3561 0.6135 -0.3058 0.5495 -0.1354 0.4274 -0.6057 0.2188 -0.0056 0.5780 -0.2528 0.4831 0.2031 0.7634 0.6817 1.0091 -0.0240 1.2469 0.4887
AZ-628 MMAC-SF 1.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.7246 -0.6528 1.1792 -1.1907 0.4608 -0.2784 0.2825 0.0925 0.4386 0.3325 0.0804 0.9901 0.1080 0.5556 -0.0758 0.7874 -0.8445 0.9346 -0.0866 1.4451 0.2299 0.5556 -0.6120 0.4695 -0.2179 0.4630 -0.6363 0.2268 0.0585 0.5525 -0.3603 0.5076 0.2244 0.7937 0.6598 1.3812 0.3491 1.3280 0.5136
AZ-628 MMAC-SF 1.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.6993 -0.6528 1.0000 -1.1907 0.4405 -0.2784 0.2315 0.0925 0.3937 0.3325 0.6135 0.0804 0.8696 0.1080 0.4717 -0.0758 0.6211 -0.8445 0.9009 -0.0866 1.2346 0.2299 0.4546 -0.6120 0.4386 -0.2179 0.4695 -0.6363 0.2179 0.0585 -0.3603 0.2244 0.6944 0.6598 1.4205 0.3491 1.0799 0.5136
AZ-628 MMAC-SF 1.0000
uM
 10
hr
 3
Odyssey/Microvigene
 0.6849 -0.6528 1.1723 -1.1907 0.4292 -0.2784 0.2083 0.0925 0.4149 0.3325 0.6410 0.0804 0.8475 0.1080 0.5376 -0.0758 0.6579 -0.8445 0.8197 -0.0866 1.3316 0.2299 0.5376 -0.6120 0.4274 -0.2179 0.4608 -0.6363 0.0585 0.5952 -0.3603 0.4902 0.2244 0.6944 0.6598 1.2739 0.3491 1.2285 0.5136
AZ-628 MMAC-SF 1.0000
uM
 10
hr
 4
Odyssey/Microvigene
 0.7246 -0.6528 1.1507 -1.1907 0.4808 -0.2784 0.2604 0.0925 0.4082 0.3325 0.6369 0.0804 1.0309 0.1080 0.5000 -0.0758 0.6803 -0.8445 0.8929 -0.0866 1.4144 0.2299 0.4608 -0.6120 0.5076 -0.2179 0.4831 -0.6363 0.2141 0.0585 0.5525 -0.3603 0.4951 0.2244 0.7092 0.6598 1.3055 0.3491 1.2300 0.5136
AZ-628 MMAC-SF 1.0000
uM
 10
hr
 5
Odyssey/Microvigene
 0.6329 -0.6528 1.0482 -1.1907 0.4184 -0.2784 0.2268 0.0925 0.4016 0.3325 0.6623 0.0804 0.9009 0.1080 0.5405 -0.0758 0.7194 -0.8445 0.8403 -0.0866 1.3351 0.2299 0.4630 -0.6120 0.4310 -0.2179 0.4329 -0.6363 0.2179 0.0585 0.5495 -0.3603 0.4808 0.2244 0.6667 0.6598 1.2469 0.3491 1.1521 0.5136
AZ-628 MMAC-SF 1.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.5988 -0.6528 1.0267 -1.1907 0.4673 -0.2784 0.2188 0.0925 0.3759 0.3325 0.5376 0.0804 0.9259 0.1080 0.4673 -0.0758 0.6944 -0.8445 0.8547 -0.0866 1.3263 0.2299 0.3774 -0.6120 0.4329 -0.2179 0.4310 -0.6363 0.2128 0.0585 0.5814 -0.3603 0.4926 0.2244 0.6135 0.6598 1.2210 0.3491 1.1338 0.5136
AZ-628 MMAC-SF 1.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.6369 -0.6528 1.1429 -1.1907 0.4202 -0.2784 0.2469 0.0925 0.3718 0.3325 0.6173 0.0804 0.8772 0.1080 0.5495 -0.0758 0.6849 -0.8445 0.9091 -0.0866 1.3908 0.2299 0.4651 -0.6120 0.4525 -0.2179 0.4785 -0.6363 0.2137 0.0585 0.5988 -0.3603 0.4975 0.2244 0.6024 0.6598 1.2837 0.3491 1.0929 0.5136
AZ-628 MMAC-SF 1.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.7246 -0.6528 1.3038 -1.1907 0.5405 -0.2784 0.2688 0.0925 0.4717 0.3325 0.6897 0.0804 1.0320 0.1080 0.5848 -0.0758 0.7143 -0.8445 0.9524 -0.0866 1.3441 0.2299 0.5714 -0.6120 0.5181 -0.2179 0.4739 -0.6363 0.2227 0.0585 0.5319 -0.3603 0.5025 0.2244 0.7692 0.6598 1.3298 0.3491 1.2739 0.5136
AZ-628 MMAC-SF 1.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.8265 -0.6072 1.3477 -0.9627 0.5291 -0.1243 0.2494 0.0410 0.4673 0.5126 0.7813 0.2579 0.9901 0.1412 0.5291 -0.1364 0.5376 -1.3476 0.9901 0.0419 1.5129 0.3604 0.6061 -0.2355 0.5814 0.0650 0.5917 -0.4788 0.2212 -0.0313 0.5319 -0.3683 0.5076 0.2244 0.7692 0.7591 0.5952 -1.0233 1.6474 0.9104
AZ-628 MMAC-SF 1.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.6410 -0.6072 1.1136 -0.9627 0.4630 -0.1243 0.2288 0.0410 0.5126 0.6667 0.2579 0.8547 0.1412 0.4255 -0.1364 0.4651 -1.3476 0.9901 0.0419 1.4388 0.3604 0.5618 -0.2355 0.4808 0.0650 0.4444 -0.4788 0.1908 -0.0313 -0.3683 0.5682 0.2244 0.7194 0.7591 0.4505 -1.0233 1.3587 0.9104
AZ-628 MMAC-SF 1.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.6536 -0.6072 1.2151 -0.9627 0.4926 -0.1243 0.1845 0.0410 0.4329 0.5126 0.7576 0.2579 0.8850 0.1412 0.5465 -0.1364 0.4762 -1.3476 0.9259 0.0419 1.3680 0.3604 0.5780 -0.2355 0.5181 0.0650 0.4630 -0.4788 0.2232 -0.0313 0.5556 -0.3683 0.4651 0.2244 0.6944 0.7591 0.4386 -1.0233 1.5015 0.9104
AZ-628 MMAC-SF 1.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.7092 -0.6072 1.3387 -0.9627 0.5376 -0.1243 0.2326 0.0410 0.4444 0.5126 0.7752 0.2579 0.9804 0.1412 0.5051 -0.1364 0.4854 -1.3476 0.9259 0.0419 1.3569 0.3604 0.6024 -0.2355 0.4673 0.0650 0.5435 -0.4788 0.2062 -0.0313 0.5556 -0.3683 0.5319 0.2244 0.8065 0.7591 0.5000 -1.0233 1.5432 0.9104
AZ-628 MMAC-SF 1.0000
uM
 24
hr
 5
Odyssey/Microvigene
 0.7576 -0.6072 1.3605 -0.9627 0.4630 -0.1243 0.2033 0.0410 0.4651 0.5126 0.6803 0.2579 0.9615 0.1412 0.5650 -0.1364 0.4902 -1.3476 0.9709 0.0419 1.5083 0.3604 0.5882 -0.2355 0.5465 0.0650 0.4115 -0.4788 0.2033 -0.0313 0.5435 -0.3683 0.4630 0.2244 0.7042 0.7591 0.5000 -1.0233 1.5924 0.9104
AZ-628 MMAC-SF 1.0000
uM
 24
hr
 6
Odyssey/Microvigene
 0.6494 -0.6072 1.1710 -0.9627 0.5102 -0.1243 0.2222 0.0410 0.4184 0.5126 0.6579 0.2579 0.9346 0.1412 0.4149 -0.1364 0.4785 -1.3476 0.9901 0.0419 1.4859 0.3604 -0.2355 0.5435 0.0650 0.4975 -0.4788 0.1949 -0.0313 0.6135 -0.3683 0.4951 0.2244 0.7692 0.7591 0.4587 -1.0233 1.4970 0.9104