Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
LOXIMVI 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0925 0.0790 0.0533 0.0055 0.0233 0.0278 0.0036 0.0054 0.0405 0.0142 0.0233 0.1672 0.0179 0.0121 0.0048 0.0533 0.0131 0.0085 0.1799 0.0673
LOXIMVI 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.1017 0.1618 0.0808 0.0068 0.0231 0.0379 0.0050 0.0060 0.0373 0.0222 0.0422 0.1712 0.0187 0.0190 0.0052 0.0614 0.0202 0.0103 0.2294 0.0987
LOXIMVI 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0999 0.1527 0.0869 0.0075 0.0226 0.0343 0.0046 0.0060 0.0382 0.0207 0.0450 0.1712 0.0181 0.0203 0.0048 0.0631 0.0163 0.0087 0.1845 0.0646
LOXIMVI 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0812 0.1085 0.0649 0.0078 0.0164 0.0275 0.0027 0.0040 0.0341 0.0142 0.0307 0.1263 0.0135 0.0114 0.0044 0.0480 0.0109 0.0088 0.1669 0.0597
LOXIMVI 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0756 0.1166 0.0639 0.0068 0.0183 0.0234 0.0031 0.0049 0.0362 0.0132 0.0312 0.1332 0.0139 0.0114 0.0045 0.0491 0.0105 0.0065 0.1333 0.0608
LOXIMVI 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0816 0.1454 0.0764 0.0072 0.0161 0.0313 0.0034 0.0047 0.0423 0.0190 0.0415 0.1418 0.0150 0.0131 0.0054 0.0489 0.0141 0.0081 0.1923 0.0870
LOXIMVI 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0775 0.1377 0.0742 0.0068 0.0162 0.0268 0.0034 0.0059 0.0439 0.0204 0.0411 0.1328 0.0157 0.0142 0.0053 0.0521 0.0121 0.0071 0.1513 0.0688
LOXIMVI 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0736 0.0878 0.0544 0.0069 0.0129 0.0263 0.0025 0.0039 0.0369 0.0134 0.0214 0.1148 0.0136 0.0107 0.0040 0.0450 0.0100 0.0068 0.1458 0.0556
LOXIMVI 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0713 0.0725 0.0618 0.0170 0.0255 0.0032 0.0049 0.0369 0.0139 0.0226 0.1247 0.0142 0.0105 0.0054 0.0485 0.0096 0.0063 0.1351 0.0588
LOXIMVI 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0789 0.1133 0.0701 0.0058 0.0300 0.0032 0.0047 0.0342 0.0331 0.1486 0.0149 0.0136 0.0038 0.0503 0.0141 0.0092 0.1949 0.0718
LOXIMVI 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0735 0.1010 0.0703 0.0064 0.0174 0.0254 0.0033 0.0049 0.0326 0.0146 0.0285 0.1314 0.0131 0.0144 0.0038 0.0484 0.0129 0.0071 0.1701 0.0543
LOXIMVI 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0677 0.0736 0.0548 0.0058 0.0162 0.0245 0.0023 0.0035 0.0326 0.0121 0.0224 0.1191 0.0125 0.0108 0.0458 0.0099 0.0073 0.1490 0.0526
LOXIMVI 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0694 0.0720 0.0533 0.0050 0.0154 0.0242 0.0027 0.0048 0.0274 0.0108 0.0220 0.1106 0.0109 0.0116 0.0039 0.0470 0.0108 0.0058 0.1183 0.0539
LOXIMVI 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0744 0.1252 0.0706 0.0079 0.0175 0.0285 0.0026 0.0037 0.0426 0.0159 0.0299 0.1397 0.0136 0.0121 0.0039 0.0468 0.0118 0.0080 0.2092 0.0618
LOXIMVI 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0799 0.1138 0.0679 0.0155 0.0244 0.0029 0.0047 0.0416 0.0149 0.0273 0.1229 0.0134 0.0121 0.0040 0.0455 0.0112 0.0062 0.1795 0.0538
LOXIMVI 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0856 0.0719 0.0500 0.0064 0.0165 0.0298 0.0027 0.0038 0.0409 0.0128 0.0190 0.1468 0.0170 0.0144 0.0041 0.0583 0.0108 0.0079 0.1595 0.0636
LOXIMVI 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0987 0.0687 0.0546 0.0054 0.0177 0.0263 0.0029 0.0056 0.0390 0.0127 0.0202 0.1605 0.0189 0.0131 0.0040 0.0636 0.0128 0.0067 0.1435 0.0690
LOXIMVI 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.1120 0.0708 0.0061 0.0364 0.0023 0.0040 0.0516 0.0161 0.0186 0.1645 0.0178 0.0144 0.0031 0.0710 0.0109 0.0095 0.1667 0.0796
LOXIMVI 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.1076 0.0668 0.0562 0.0054 0.0203 0.0253 0.0025 0.0046 0.0430 0.0122 0.0167 0.1297 0.0139 0.0152 0.0032 0.0579 0.0100 0.0085 0.1539 0.0561
LOXIMVI 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.1087 0.0885 0.0587 0.0066 0.0234 0.0274 0.0024 0.0038 0.0514 0.0129 0.0227 0.1724 0.0158 0.0146 0.0040 0.0615 0.0111 0.0102 0.1669 0.1263
LOXIMVI 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.1103 0.0748 0.0522 0.0053 0.0225 0.0217 0.0031 0.0052 0.0387 0.0107 0.0212 0.1508 0.0151 0.0120 0.0039 0.0697 0.0112 0.0095 0.1536 0.0953
LOXIMVI 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0842 0.0861 0.0551 0.0064 0.0196 0.0305 0.0024 0.0041 0.0490 0.0126 0.0210 0.1421 0.0162 0.0138 0.0033 0.0569 0.0097 0.0071 0.1585 0.0635
LOXIMVI 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0873 0.0829 0.0531 0.0180 0.0233 0.0027 0.0045 0.0419 0.0131 0.0206 0.1144 0.0130 0.0133 0.0033 0.0509 0.0097 0.0064 0.1550 0.0451
LOXIMVI 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.1006 0.0987 0.0537 0.0063 0.0211 0.0301 0.0029 0.0039 0.0566 0.0138 0.0247 0.1754 0.0157 0.0036 0.0653 0.0112 0.0077 0.1241
LOXIMVI 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.1122 0.0934 0.0547 0.0061 0.0211 0.0221 0.0032 0.0053 0.0411 0.0142 0.0210 0.1529 0.0152 0.0148 0.0039 0.0656 0.0122 0.0081 0.1647 0.0842