Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
LOXIMVI 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0934 0.2500 0.1172 0.0140 0.0173 0.0395 0.0042 0.0097 0.5882 0.0191 0.0510 0.4065 0.0337 0.0334 0.0047 0.0739 0.0179 0.0116 0.4464 0.1042
LOXIMVI 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.1037 0.2294 0.1144 0.0129 0.0178 0.0308 0.0051 0.0132 0.5076 0.0193 0.0500 0.3195 0.0296 0.0311 0.0057 0.0743 0.0208 0.0100 0.4219 0.0625
LOXIMVI 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0891 0.1201 0.0706 0.0094 0.0125 0.0305 0.0043 0.0065 0.4425 0.0132 0.0246 0.2793 0.0256 0.0232 0.0040 0.0622 0.0109 0.0076 0.2907 0.0475
LOXIMVI 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0984 0.1110 0.0688 0.0095 0.0134 0.0250 0.0040 0.0090 0.2941 0.0127 0.0248 0.2732 0.0253 0.0219 0.0044 0.0629 0.0146 0.0079 0.2688 0.0483
LOXIMVI 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.1495 0.3106 0.1181 0.0157 0.0167 0.0563 0.0069 0.0131 0.5263 0.0345 0.0645 0.3922 0.0427 0.0610 0.0064 0.0900 0.0251 0.0110 0.4348 0.1490
LOXIMVI 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.1592 0.3205 0.1274 0.0131 0.0176 0.0475 0.0069 0.0165 0.5076 0.0419 0.0695 0.3677 0.0392 0.0693 0.0080 0.0936 0.0250 0.0117 0.4082 0.1081
LOXIMVI 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.1177 0.1707 0.0958 0.0107 0.0109 0.0375 0.0035 0.0065 0.4975 0.0224 0.0441 0.2865 0.0320 0.0302 0.0052 0.0713 0.0141 0.0086 0.3663 0.0965
LOXIMVI 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.1178 0.1754 0.0999 0.0105 0.0119 0.0309 0.0035 0.0089 0.5376 0.0231 0.0431 0.3663 0.0344 0.0354 0.0062 0.0705 0.0129 0.0078 0.3509 0.0781
LOXIMVI 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.1337 0.2825 0.1192 0.0133 0.0178 0.0599 0.0068 0.0129 0.4630 0.0244 0.0703 0.4115 0.0414 0.0360 0.0080 0.0856 0.0265 0.0114 0.4566 0.1330
LOXIMVI 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.1395 0.2732 0.1276 0.0112 0.0190 0.0487 0.0072 0.0182 0.4831 0.0280 0.0726 0.4329 0.0387 0.0407 0.0086 0.0903 0.0283 0.0126 0.4237 0.1065
LOXIMVI 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.1047 0.1468 0.0852 0.0093 0.0110 0.0359 0.0035 0.0063 0.4049 0.0152 0.0347 0.2695 0.0264 0.0205 0.0044 0.0647 0.0125 0.0079 0.3021 0.0811
LOXIMVI 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0956 0.1332 0.0843 0.0089 0.0115 0.0319 0.0037 0.0089 0.3891 0.0140 0.0352 0.3175 0.0283 0.0173 0.0056 0.0797 0.0151 0.0086 0.2924 0.0701
LOXIMVI 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.1250 0.2611 0.1131 0.0120 0.0147 0.0447 0.0053 0.0104 0.5208 0.0298 0.0539 0.3356 0.0350 0.0437 0.0054 0.0837 0.0186 0.0104 0.4484
LOXIMVI 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.1236 0.2481 0.1166 0.0114 0.0142 0.0412 0.0047 0.0098 0.5319 0.0291 0.0530 0.3226 0.0342 0.0479 0.0053 0.0809 0.0167 0.0087 0.3521 0.0688
LOXIMVI 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0917 0.1395 0.0811 0.0090 0.0094 0.0291 0.0028 0.0056 0.3937 0.0204 0.0286 0.2519 0.0252 0.0285 0.0043 0.0622 0.0105 0.0075 0.2801 0.0689
LOXIMVI 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0856 0.1351 0.0724 0.0086 0.0104 0.0258 0.0032 0.0078 0.4032 0.0174 0.0291 0.2433 0.0246 0.0251 0.0041 0.0626 0.0100 0.0058 0.2237 0.0714
LOXIMVI 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0989 0.2370 0.1117 0.0101 0.0149 0.0404 0.0047 0.0112 0.4425 0.0205 0.0589 0.3205 0.0309 0.0280 0.0064 0.0729 0.0204 0.0102 0.3731 0.0903
LOXIMVI 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0966 0.2105 0.1109 0.0121 0.0148 0.0358 0.0051 0.0116 0.4367 0.0198 0.0568 0.3003 0.0324 0.0299 0.0061 0.0766 0.0166 0.0085 0.2959 0.0735
LOXIMVI 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0803 0.1307 0.0732 0.0073 0.0096 0.0280 0.0028 0.0053 0.3226 0.0120 0.0289 0.2237 0.0231 0.0170 0.0041 0.0613 0.0115 0.0079 0.2488 0.0562
LOXIMVI 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0765 0.1072 0.0814 0.0084 0.0115 0.0262 0.0036 0.0077 0.3155 0.0120 0.0299 0.2421 0.0251 0.0172 0.0047 0.0562 0.0123 0.0068 0.2283 0.0640
LOXIMVI 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.1078 0.1984 0.1027 0.0106 0.0229 0.0375 0.0040 0.0080 0.4808 0.0271 0.0469 0.2976 0.0289 0.0389 0.0044 0.0719 0.0166 0.0100 0.3663 0.0850
LOXIMVI 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.1033 0.1727 0.1016 0.0105 0.0111 0.0329 0.0041 0.0086 0.4237 0.0218 0.0382 0.2591 0.0255 0.0399 0.0039 0.0699 0.0137 0.0070 0.3247 0.0560
LOXIMVI 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0858 0.1462 0.0769 0.0092 0.0107 0.0293 0.0028 0.0050 0.4237 0.0165 0.0287 0.2278 0.0228 0.0235 0.0036 0.0625 0.0107 0.0078 0.2865 0.0637
LOXIMVI 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0890 0.1333 0.0748 0.0088 0.0104 0.0251 0.0031 0.0076 0.3846 0.0157 0.0268 0.2165 0.0206 0.0258 0.0042 0.0639 0.0114 0.0064 0.2532 0.0611
LOXIMVI 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.1006 0.2506 0.1174 0.0120 0.0175 0.0420 0.0044 0.0090 0.4762 0.0227 0.0568 0.3497 0.0293 0.0287 0.0051 0.0739 0.0189 0.0104 0.4546 0.0838