Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
LOXIMVI 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.1311 1.0309 0.1151 0.0214 0.0404 0.0761 0.0101 0.0148 0.8475 0.0461 0.1256 0.6803 0.0831 0.0829 0.0091 0.0881 0.0519 0.0303 0.2660 0.2584
LOXIMVI 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.1366 0.9615 0.1166 0.0248 0.0408 0.0719 0.0136 0.0185 0.6849 0.0608 0.1188 0.5747 0.0661 0.0852 0.0116 0.1006 0.0506 0.0217 0.2488 0.1848
LOXIMVI 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.0633 0.3195 0.0514 0.0105 0.0244 0.0286 0.0031 0.0040 0.5051 0.0183 0.0459 0.2770 0.0252 0.0275 0.0042 0.0430 0.0124 0.0093 0.0968 0.0689
LOXIMVI 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.0769 0.3145 0.0542 0.0215 0.0157 0.0268 0.0052 0.0065 0.3509 0.0169 0.0463 0.2653 0.0258 0.0370 0.0069 0.0457 0.0225 0.0103 0.1013 0.0821
LOXIMVI 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0954 0.4717 0.0784 0.0170 0.0195 0.0406 0.0067 0.0093 0.8065 0.0311 0.0627 0.4717 0.0505 0.0462 0.0063 0.0574 0.0265 0.0151 0.2174 0.2825
LOXIMVI 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.1198 0.4673 0.0770 0.0160 0.0172 0.0317 0.0078 0.0122 0.7299 0.0310 0.0631 0.3774 0.0471 0.0528 0.0090 0.0564 0.0437 0.0174 0.2146 0.1294
LOXIMVI 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0828 0.3185 0.0541 0.0116 0.0157 0.0265 0.0037 0.0058 0.6211 0.0185 0.0451 0.3650 0.0329 0.0342 0.0051 0.0438 0.0165 0.0105 0.1250 0.1511
LOXIMVI 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0799 0.3003 0.0463 0.0112 0.0154 0.0218 0.0046 0.0074 0.4717 0.0201 0.0408 0.2793 0.0278 0.0321 0.0062 0.0417 0.0194 0.0115 0.1247 0.0864
LOXIMVI 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.1393 0.9259 0.1326 0.0255 0.0309 0.0561 0.0096 0.0140 0.9709 0.0440 0.1031 0.7353 0.0718 0.0834 0.0090 0.0758 0.0480 0.0216 0.2237 0.3731
LOXIMVI 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.1441 0.8403 0.1120 0.0247 0.0368 0.0459 0.0126 0.0183 0.7692 0.0377 0.0991 0.5236 0.0626 0.0799 0.0762 0.0615 0.0227 0.2128 0.1873
LOXIMVI 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0780 0.3704 0.0546 0.0127 0.0187 0.0270 0.0038 0.0054 0.5587 0.0162 0.0446 0.3257 0.0288 0.0373 0.0049 0.0433 0.0139 0.0104 0.1011 0.1284
LOXIMVI 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0791 0.3290 0.0512 0.0126 0.0180 0.0223 0.0053 0.0075 0.4032 0.0164 0.0402 0.2681 0.0259 0.0280 0.0059 0.0433 0.0210 0.0106 0.1002 0.0928
LOXIMVI 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.1560 0.7463 0.0947 0.0153 0.0272 0.0680 0.0109 0.0158 0.7042 0.0540 0.1121 0.6369 0.0859 0.0692 0.0104 0.0894 0.0453 0.0239 0.2865 0.3584
LOXIMVI 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.1631 0.7463 0.1006 0.0160 0.0282 0.0624 0.0092 0.0156 0.7407 0.0622 0.1218 0.6135 0.0758 0.0774 0.0116 0.0946 0.0471 0.0237 0.2387 0.2247
LOXIMVI 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0763 0.2740 0.0452 0.0088 0.0108 0.0276 0.0033 0.0049 0.4566 0.0196 0.0433 0.2857 0.0322 0.0233 0.0055 0.0434 0.0123 0.0107 0.1153 0.1880
LOXIMVI 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0666 0.2778 0.0441 0.0120 0.0124 0.0264 0.0033 0.0058 0.4878 0.0190 0.0434 0.2841 0.0320 0.0239 0.0059 0.0452 0.0120 0.0088 0.0985 0.1346
LOXIMVI 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.1764 1.0309 0.1212 0.0263 0.0375 0.0901 0.0130 0.0149 0.8333 0.0488 0.1368 0.7143 0.0820 0.0693 0.0159 0.1075 0.0584 0.0249 0.2506 0.3802
LOXIMVI 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.1698 1.0000 0.1344 0.0215 0.0408 0.0870 0.0112 0.0180 0.8929 0.0553 0.1466 0.6897 0.0868 0.0793 0.0176 0.1082 0.0568 0.0225 0.2079 0.2667
LOXIMVI 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0814 0.3413 0.0538 0.0110 0.0145 0.0310 0.0037 0.0055 0.4762 0.0175 0.0467 0.2985 0.0287 0.0355 0.0058 0.0483 0.0135 0.0106 0.0912 0.1479
LOXIMVI 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0699 0.2950 0.0613 0.0107 0.0172 0.0291 0.0046 0.0063 0.4348 0.0151 0.0460 0.3165 0.0293 0.0292 0.0070 0.0472 0.0179 0.0105 0.0840 0.1226
LOXIMVI 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.1300 0.8850 0.1036 0.0150 0.0294 0.0791 0.0125 0.0142 0.6289 0.0563 0.1205 0.6329 0.0870 0.0363 0.0094 0.0958 0.0481 0.0242 0.2933 0.2304
LOXIMVI 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.1350 0.8065 0.1074 0.0158 0.0266 0.0627 0.0098 0.0135 0.7092 0.0521 0.1135 0.6211 0.0785 0.0706 0.0087 0.1008 0.0343 0.0193 0.2336 0.1580
LOXIMVI 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0697 0.3690 0.0531 0.0100 0.0137 0.0292 0.0036 0.0049 0.4425 0.0233 0.0529 0.3049 0.0332 0.0266 0.0057 0.0454 0.0150 0.0114 0.1131 0.1198
LOXIMVI 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0679 0.3571 0.0534 0.0103 0.0142 0.0280 0.0046 0.0064 0.4587 0.0235 0.0534 0.2959 0.0322 0.0275 0.0060 0.0475 0.0171 0.0092 0.0940 0.1224
LOXIMVI 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.1027 0.8065 0.0969 0.0191 0.0301 0.0541 0.0075 0.0106 0.5917 0.0339 0.0992 0.5181 0.0601 0.0535 0.0094 0.0709 0.0332 0.0231 0.1996 0.1709