Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.1058 0.4902 0.0132 0.0120 0.0138 0.1314 0.0232 0.1773 0.0348 0.0345 0.1980 0.0232 0.0597 0.0096 0.0546 0.0181 0.1049 0.0261
WM1552C 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.0844 0.4505 0.0113 0.0126 0.0150 0.1284 0.0225 0.1300 0.0480 0.0290 0.1681 0.0260 0.0515 0.0070 0.0471 0.0138 0.0919
WM1552C 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.0888 0.4255 0.0109 0.0146 0.0135 0.1161 0.0236 0.1152 0.0407 0.0281 0.1707 0.0233 0.0551 0.0080 0.0431 0.0143 0.1079 0.0264
WM1552C 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.0827 0.3788 0.0084 0.0099 0.0103 0.0720 0.0158 0.0914 0.0223 0.0235 0.0962 0.0147 0.0403 0.0063 0.0287 0.0102 0.0612 0.0203
WM1552C 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.0886 0.3367 0.0097 0.0103 0.0144 0.0867 0.0157 0.0929 0.0196 0.0267 0.1068 0.0146 0.0393 0.0070 0.0245 0.0107 0.0600 0.0199
WM1552C 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.1070 0.5952 0.0142 0.0129 0.0110 0.1887 0.0274 0.1715 0.0442 0.0351 0.2976 0.0379 0.0847 0.0083 0.1361 0.0229 0.1319 0.0274
WM1552C 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.1114 0.5618 0.0121 0.0118 0.0187 0.1898 0.0291 0.1563 0.0439 0.0342 0.2985 0.0336 0.0841 0.0098 0.0958 0.0257 0.1451 0.0250
WM1552C 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.1002 0.5465 0.0113 0.0115 0.0117 0.1409 0.0249 0.1410 0.0409 0.0304 0.2359 0.0269 0.0720 0.0085 0.0682 0.0163 0.1353 0.0243
WM1552C 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.1086 0.5525 0.0138 0.0129 0.0137 0.1751 0.0295 0.1905 0.0426 0.0384 0.2500 0.0272 0.0618 0.0097 0.0664 0.0187 0.1294 0.0276
WM1552C 0.0000
uM
 24
hr
 5
Odyssey/Microvigene
 0.0883 0.4926 0.0112 0.0122 0.0119 0.1621 0.0281 0.1502 0.0548 0.0288 0.2681 0.0347 0.0471 0.0080 0.0816 0.0167 0.1536 0.0324
WM1552C 0.0000
uM
 24
hr
 5
Odyssey/Microvigene
 0.0948 0.5525 0.0118 0.0155 0.0161 0.1493 0.0222 0.1637 0.0544 0.0329 0.2639 0.0327 0.0700 0.0090 0.0742 0.0232 0.1595 0.0285
WM1552C 0.0000
uM
 24
hr
 5
Odyssey/Microvigene
 0.0990 0.4808 0.0133 0.0141 0.0156 0.1355 0.0256 0.1748 0.0509 0.0275 0.1848 0.0272 0.0487 0.0077 0.0652 0.0165 0.1142 0.0590
WM1552C 0.0000
uM
 24
hr
 5
Odyssey/Microvigene
 0.1031 0.5435 0.0140 0.0162 0.0140 0.1486 0.0292 0.1779 0.0532 0.0309 0.2451 0.0233 0.0565 0.0082 0.0598 0.0172 0.1230 0.0340
WM1552C 0.0000
uM
 24
hr
 5
Odyssey/Microvigene
 0.1047 0.5780 0.0119 0.0137 0.0159 0.1707 0.0309 0.1786 0.0529 0.0329 0.2833 0.0416 0.0752 0.0080 0.0863 0.0200 0.1869 0.0330
WM1552C 0.0000
uM
 24
hr
 5
Odyssey/Microvigene
 0.1185 0.6369 0.0152 0.0165 0.0130 0.1761 0.0353 0.2012 0.0542 0.0327 0.2916 0.0342 0.0901 0.0096 0.0967 0.0266 0.2012 0.0422
WM1552C 0.0000
uM
 24
hr
 5
Odyssey/Microvigene
 0.1043 0.5181 0.0119 0.0136 0.0098 0.1333 0.0240 0.1590 0.0450 0.0293 0.2105 0.0224 0.0646 0.0075 0.0651 0.0181 0.1179 0.0475
WM1552C 0.0000
uM
 24
hr
 5
Odyssey/Microvigene
 0.1097 0.5848 0.0136 0.0148 0.0185 0.1495 0.0273 0.1739 0.0421 0.0340 0.2262 0.0247 0.0760 0.0082 0.0503 0.0167 0.1168 0.0294
WM1552C 0.0000
uM
 24
hr
 6
Odyssey/Microvigene
 0.0876 0.5128 0.0117 0.0119 0.0130 0.1707 0.0287 0.1689 0.0555 0.0300 0.2941 0.0333 0.0534 0.0071 0.1012 0.0214 0.1502 0.0300
WM1552C 0.0000
uM
 24
hr
 6
Odyssey/Microvigene
 0.0984 0.5263 0.0112 0.0142 0.0123 0.1473 0.0281 0.1376 0.0536 0.0291 0.2817 0.0305 0.0659 0.0084 0.0835 0.0236 0.1709 0.0257
WM1552C 0.0000
uM
 24
hr
 6
Odyssey/Microvigene
 0.0912 0.5181 0.0114 0.0129 0.0129 0.1391 0.0265 0.1745 0.0557 0.0273 0.2364 0.0311 0.0508 0.0083 0.0747 0.0216 0.1410 0.0343
WM1552C 0.0000
uM
 24
hr
 6
Odyssey/Microvigene
 0.0967 0.5747 0.0128 0.0145 0.0202 0.1475 0.0280 0.1754 0.0562 0.0322 0.2597 0.0262 0.0545 0.0080 0.0784 0.0192 0.1484 0.0278
WM1552C 0.0000
uM
 24
hr
 6
Odyssey/Microvigene
 0.1046 0.5714 0.0127 0.0123 0.0127 0.1862 0.0301 0.1805 0.0528 0.0321 0.3040 0.0383 0.0743 0.0081 0.1018 0.0256 0.1629
WM1552C 0.0000
uM
 24
hr
 6
Odyssey/Microvigene
 0.1146 0.6098 0.0127 0.0131 0.0188 0.1822 0.0307 0.1709 0.0499 0.0332 0.3247 0.0339 0.0829 0.0094 0.0941 0.0238 0.1779 0.0231
WM1552C 0.0000
uM
 24
hr
 6
Odyssey/Microvigene
 0.0940 0.4464 0.0106 0.0146 0.0104 0.1183 0.0208 0.1311 0.0325 0.0272 0.1695 0.0205 0.0553 0.0070 0.0482 0.0178 0.1022 0.0271
WM1552C 0.0000
uM
 24
hr
 6
Odyssey/Microvigene
 0.0979 0.4808 0.0119 0.0142 0.0168 0.1344 0.0222 0.1407 0.0312 0.0333 0.2004 0.0185 0.0575 0.0077 0.0416 0.0152 0.0958 0.0237