Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0268 0.2342 0.0487 0.0216 0.0049 0.0068 0.0037 0.0036 0.0046 0.0204 0.0640 0.0213 0.0636 0.0081 0.0044 0.0033 0.0064 0.0077 0.0048 0.0808 0.0503
WM1552C 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0401 0.4484 0.0763 0.0387 0.0106 0.0121 0.0070 0.0112 0.0106 0.0271 0.1054 0.0466 0.1129 0.0237 0.0111 0.0098 0.0123 0.0172 0.0101 0.1527 0.0665
WM1552C 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0489 0.6061 0.0857 0.0483 0.0116 0.0108 0.0073 0.0075 0.0104 0.0308 0.1252 0.0485 0.1471 0.0216 0.0111 0.0077 0.0148 0.0224 0.0111 0.1730 0.0890
WM1552C 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0254 0.1359 0.0241 0.0118 0.0035 0.0036 0.0027 0.0042 0.0036 0.0277 0.0172 0.0322 0.0071 0.0044 0.0040 0.0054 0.0048 0.0050 0.0693 0.0279
WM1552C 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0309 0.1647 0.0276 0.0131 0.0044 0.0048 0.0030 0.0042 0.0043 0.0536 0.0410 0.0194 0.0454 0.0069 0.0052 0.0037 0.0053 0.0067 0.0052 0.0786 0.0446
WM1552C 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0268 0.2985 0.0639 0.0285 0.0090 0.0093 0.0055 0.0076 0.0072 0.0284 0.0787 0.0318 0.0694 0.0135 0.0067 0.0058 0.0092 0.0130 0.0070 0.1048 0.0394
WM1552C 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0334 0.3460 0.0568 0.0308 0.0078 0.0085 0.0043 0.0057 0.0058 0.0306 0.0876 0.0289 0.0898 0.0144 0.0066 0.0059 0.0097 0.0107 0.0073 0.1103 0.0559
WM1552C 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0220 0.1629 0.0346 0.0151 0.0041 0.0040 0.0029 0.0039 0.0037 0.0480 0.0371 0.0158 0.0369 0.0072 0.0048 0.0040 0.0054 0.0055 0.0047 0.0708 0.0311
WM1552C 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0284 0.1938 0.0354 0.0178 0.0050 0.0064 0.0033 0.0035 0.0043 0.0485 0.0434 0.0168 0.0524 0.0068 0.0045 0.0040 0.0069 0.0072 0.0054 0.0791 0.0488
WM1552C 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0282 0.2976 0.0595 0.0291 0.0078 0.0068 0.0048 0.0069 0.0065 0.0264 0.0674 0.0304 0.0693 0.0138 0.0065 0.0068 0.0085 0.0109 0.0072 0.1091 0.0402
WM1552C 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0378 0.3597 0.0614 0.0351 0.0081 0.0081 0.0052 0.0065 0.0066 0.0284 0.0809 0.0299 0.0932 0.0146 0.0079 0.0054 0.0100 0.0134 0.0075 0.1171 0.0639
WM1552C 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0271 0.1522 0.0257 0.0128 0.0037 0.0036 0.0026 0.0037 0.0034 0.0756 0.0297 0.0167 0.0346 0.0075 0.0049 0.0045 0.0052 0.0048 0.0051 0.0820 0.0366
WM1552C 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0368 0.1869 0.0300 0.0164 0.0044 0.0059 0.0031 0.0043 0.0047 0.0623 0.0406 0.0180 0.0546 0.0083 0.0056 0.0052 0.0053 0.0076 0.0058 0.0862 0.0555
WM1552C 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0235 0.1795 0.0389 0.0180 0.0050 0.0049 0.0038 0.0046 0.0048 0.0538 0.0370 0.0179 0.0428 0.0085 0.0046 0.0048 0.0065 0.0079 0.0054 0.0778 0.0345
WM1552C 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0341 0.2024 0.0359 0.0195 0.0050 0.0057 0.0034 0.0041 0.0044 0.0838 0.0407 0.0157 0.0544 0.0097 0.0054 0.0040 0.0068 0.0084 0.0059 0.0826 0.0441
WM1552C 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0299 0.1580 0.0307 0.0149 0.0070 0.0044 0.0030 0.0037 0.0040 0.0944 0.0313 0.0157 0.0364 0.0071 0.0058 0.0045 0.0056 0.0057 0.0053 0.0851 0.0377
WM1552C 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0346 0.1905 0.0329 0.0161 0.0077 0.0052 0.0029 0.0032 0.0042 0.0520 0.0371 0.0165 0.0539 0.0083 0.0046 0.0034 0.0063 0.0071 0.0055 0.0962 0.0530
WM1552C 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0372 0.4000 0.0773 0.0435 0.0109 0.0101 0.0063 0.0079 0.0087 0.0345 0.1017 0.0388 0.0915 0.0222 0.0106 0.0174 0.0096 0.1276 0.0658
WM1552C 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.4695 0.0827 0.0530 0.0109 0.0116 0.0065 0.0079 0.0094 0.0362 0.1179 0.0364 0.0219 0.0062 0.0050 0.0113 0.0198 0.0107 0.1385 0.0957
WM1552C 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0319 0.1647 0.0282 0.0151 0.0040 0.0046 0.0028 0.0041 0.0042 0.0428 0.0316 0.0170 0.0407 0.0074 0.0050 0.0042 0.0057 0.0066 0.0055 0.0856 0.0455
WM1552C 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0380 0.1869 0.0296 0.0161 0.0039 0.0051 0.0032 0.0032 0.0045 0.0387 0.0398 0.0192 0.0584 0.0086 0.0048 0.0038 0.0059 0.0080 0.0057 0.0935 0.0653
WM1552C 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.0345 0.3831 0.0700 0.0382 0.0110 0.0126 0.0075 0.0103 0.0109 0.0321 0.0853 0.0394 0.0895 0.0227 0.0104 0.0077 0.0119 0.0213 0.0124 0.1076 0.0562
WM1552C 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.5291 0.0976 0.0506 0.0104 0.0127 0.0083 0.0112 0.0127 0.0379 0.1138 0.0444 0.1379 0.0253 0.0101 0.0066 0.0112 0.0223 0.0138 0.1454 0.0713
WM1552C 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.0246 0.2008 0.0375 0.0192 0.0060 0.0056 0.0042 0.0052 0.0054 0.0294 0.0402 0.0178 0.0507 0.0127 0.0065 0.0045 0.0052 0.0079 0.0067 0.0627 0.0395
WM1552C 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.0275 0.3012 0.0428 0.0208 0.0053 0.0069 0.0034 0.0051 0.0057 0.0299 0.0500 0.0221 0.0653 0.0128 0.0061 0.0042 0.0054 0.0098 0.0068 0.0707 0.0451