Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0430 0.2674 0.0023 0.0023 0.0044 0.0377 0.0470 0.0102 0.0086 0.0723 0.0084 0.0126 0.0293 0.0040 0.0064 0.0030 0.0085 0.0101 0.0028 0.0117 0.0108
WM-115 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0424 0.2976 0.0024 0.0016 0.0042 0.0354 0.0457 0.0090 0.0084 0.0817 0.0082 0.0123 0.0301 0.0043 0.0062 0.0023 0.0097 0.0086 0.0023 0.0130 0.0105
WM-115 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0525 0.2625 0.0022 0.0019 0.0096 0.0478 0.0488 0.0105 0.0094 0.0805 0.0108 0.0150 0.0361 0.0058 0.0066 0.0032 0.0062 0.0138 0.0023 0.0131 0.0121
WM-115 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0521 0.3058 0.0028 0.0013 0.0088 0.0463 0.0408 0.0095 0.0087 0.0918 0.0101 0.0149 0.0332 0.0062 0.0049 0.0022 0.0062 0.0111 0.0023 0.0141 0.0081
WM-115 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0441 0.2755 0.0026 0.0025 0.0034 0.0388 0.0626 0.0092 0.0097 0.0710 0.0068 0.0120 0.0326 0.0041 0.0061 0.0026 0.0095 0.0075 0.0028 0.0119 0.0125
WM-115 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0492 0.3058 0.0026 0.0019 0.0044 0.0391 0.0672 0.0088 0.0092 0.0858 0.0075 0.0124 0.0328 0.0047 0.0057 0.0020 0.0090 0.0082 0.0026 0.0121 0.0121
WM-115 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0527 0.2849 0.0025 0.0022 0.0056 0.0430 0.0779 0.0140 0.0104 0.0614 0.0084 0.0143 0.0378 0.0052 0.0076 0.0031 0.0096 0.0114 0.0029 0.0114 0.0155
WM-115 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0525 0.3165 0.0030 0.0020 0.0052 0.0370 0.0642 0.0105 0.0092 0.0719 0.0066 0.0133 0.0361 0.0054 0.0075 0.0025 0.0097 0.0090 0.0110 0.0166
WM-115 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0445 0.2849 0.0021 0.0020 0.0330 0.0743 0.0104 0.0095 0.0702 0.0062 0.0140 0.0313 0.0047 0.0061 0.0030 0.0106 0.0085 0.0032 0.0128 0.0109
WM-115 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0473 0.3086 0.0023 0.0019 0.0058 0.0313 0.0473 0.0073 0.0106 0.0784 0.0064 0.0097 0.0299 0.0050 0.0058 0.0026 0.0081 0.0076 0.0031 0.0133 0.0189
WM-115 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0498 0.2809 0.0023 0.0020 0.0052 0.0387 0.0883 0.0126 0.0101 0.0659 0.0084 0.0112 0.0368 0.0049 0.0063 0.0028 0.0129 0.0084 0.0035 0.0097 0.0120
WM-115 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0526 0.3257 0.0028 0.0019 0.0051 0.0351 0.0614 0.0084 0.0097 0.0723 0.0086 0.0115 0.0324 0.0050 0.0049 0.0030 0.0087 0.0096 0.0030 0.0104 0.0146
WM-115 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0608 0.2786 0.0026 0.0022 0.0052 0.0777 0.0109 0.0109 0.0617 0.0089 0.0153 0.0379 0.0058 0.0061 0.0026 0.0118 0.0096 0.0032 0.0167 0.0230
WM-115 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0554 0.3077 0.0026 0.0023 0.0062 0.0379 0.0655 0.0090 0.0105 0.0810 0.0079 0.0118 0.0346 0.0052 0.0061 0.0027 0.0092 0.0105 0.0028 0.0154
WM-115 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0483 0.2247 0.0028 0.0020 0.0030 0.0272 0.0668 0.0136 0.0085 0.0693 0.0126 0.0253 0.0061 0.0027 0.0081 0.0117 0.0026 0.0078 0.0225
WM-115 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0376 0.2632 0.0029 0.0016 0.0033 0.0251 0.0658 0.0115 0.0086 0.0860 0.0089 0.0130 0.0290 0.0039 0.0053 0.0025 0.0101 0.0083 0.0031 0.0083 0.0147
WM-115 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0431 0.2288 0.0025 0.0016 0.0030 0.0235 0.0723 0.0118 0.0095 0.0597 0.0086 0.0190 0.0247 0.0033 0.0057 0.0028 0.0086 0.0076 0.0024 0.0090 0.0215
WM-115 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0421 0.2611 0.0025 0.0020 0.0039 0.0256 0.0712 0.0145 0.0099 0.0640 0.0087 0.0132 0.0259 0.0038 0.0053 0.0031 0.0099 0.0098 0.0021 0.0116 0.0288
WM-115 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0404 0.1883 0.0025 0.0018 0.0030 0.0183 0.0577 0.0091 0.0081 0.0632 0.0092 0.0160 0.0305 0.0038 0.0030 0.0029 0.0060 0.0097 0.0027 0.0081 0.0141
WM-115 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0336 0.2169 0.0026 0.0014 0.0036 0.0185 0.0539 0.0075 0.0081 0.0780 0.0082 0.0104 0.0316 0.0035 0.0029 0.0069 0.0077 0.0093 0.0208
WM-115 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0403 0.1466 0.0028 0.0017 0.0026 0.0212 0.0687 0.0133 0.0082 0.0579 0.0075 0.0072 0.0301 0.0032 0.0059 0.0023 0.0083 0.0065 0.0024 0.0103 0.0212
WM-115 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0428 0.1681 0.0034 0.0018 0.0048 0.0248 0.0126 0.0090 0.0546 0.0072 0.0088 0.0326 0.0038 0.0053 0.0028 0.0105 0.0096 0.0024 0.0114 0.0367
WM-115 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0454 0.2770 0.0021 0.0015 0.0056 0.0292 0.0511 0.0127 0.0097 0.0704 0.0102 0.0167 0.0248 0.0046 0.0067 0.0020 0.0084 0.0022 0.0157 0.0140
WM-115 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0417 0.3125 0.0027 0.0014 0.0048 0.0283 0.0394 0.0123 0.0095 0.0821 0.0082 0.0210 0.0244 0.0034 0.0069 0.0020 0.0104 0.0109 0.0019 0.0118
WM-115 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0391 0.2841 0.0022 0.0034 0.0304 0.0542 0.0138 0.0087 0.0791 0.0084 0.0184 0.0233 0.0029 0.0062 0.0029 0.0084 0.0087 0.0021 0.0102 0.0139