Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
MZ7-mel 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.7634 2.2831 0.3571 0.2451 0.2611 0.2933 0.5780 0.5952 1.8349 0.5780 0.5208 0.2695 0.3012 0.4525 0.2024 0.3817 0.3226 1.6556 0.7143
MZ7-mel 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.8000 2.9070 0.4630 0.3155 0.2865 0.6494 0.8696 2.1834 0.7246 0.8621 0.3623 0.3876 0.2079 0.4926 0.3906 1.9685 1.0152
MZ7-mel 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.6849 2.1930 0.3774 0.2674 0.2667 0.2994 0.5682 0.5587 1.8382 0.4739 0.5348 0.2976 0.3497 0.4484 0.1923 0.2710 0.3268 1.1765 0.7246
MZ7-mel 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.6667 2.5000 0.3610 0.2625 0.2404 0.3086 0.6944 0.7092 1.8083 0.5348 0.4878 0.2890 0.3413 0.4717 0.1927 0.2786 0.3030 1.5152 0.6623
MZ7-mel 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.6944 2.2779 0.3521 0.2688 0.2695 0.3106 0.5917 0.6135 1.8692 0.5319 0.5051 0.2976 0.3012 0.4367 0.1938 0.2985 0.3003 1.4124 0.6757
MZ7-mel 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.7143 3.0120 0.4831 0.2717 0.2778 0.3571 1.0288 0.8130 2.1008 0.6098 0.7092 0.3610 0.4082 0.5435 0.2066 0.3279 0.3521 1.9380 0.7246
MZ7-mel 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.7752 3.0395 0.3759 0.2364 0.3247 0.3175 0.5618 0.6329 2.2573 0.7407 0.6494 0.2959 0.3268 0.5263 0.1842 0.3205 0.3876 1.5244 0.7519
MZ7-mel 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.7752 0.4202 0.2545 0.3145 0.3584 0.9434 0.7299 2.3866 0.7576 0.7519 0.3049 0.3484 0.5128 0.1942 0.3610 0.4049 2.2371 0.7353
MZ7-mel 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.7634 2.4450 0.3521 0.2208 0.2463 0.3125 0.5747 0.7246 1.9194 0.6993 0.5208 0.2571 0.2959 0.4525 0.1887 0.2591 0.2933 1.4006 0.6289
MZ7-mel 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.7937 2.9155 0.4525 0.2618 0.3718 0.3584 1.0000 0.9434 2.5840 0.8621 0.7463 0.3472 0.4219 0.6289 0.2037 0.4237 0.4237 2.2321 0.7752
MZ7-mel 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.6849 2.3474 0.3984 0.2538 0.3175 0.2994 0.5376 0.6452 1.9231 0.4975 0.5988 0.3367 0.3185 0.4464 0.3401 0.3460 1.5267 0.7634
MZ7-mel 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.7143 2.2573 0.4310 0.2494 0.2882 0.3049 0.6173 0.5780 1.8484 0.5000 0.5556 0.3663 0.3311 0.4785 0.2110 0.2874 0.3559 1.6026 0.7092
MZ7-mel 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.6173 1.7452 0.3623 0.2632 0.2924 0.5405 0.4878 1.6051 0.4425 0.4695 0.3086 0.2801 0.3846 0.2618 0.2639 1.2034 0.8000
MZ7-mel 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.8696 1.9417 0.4000 0.3247 0.3300 0.6897 0.5988 1.7301 0.5263 0.5025 0.3448 0.3279 0.4739 0.3115 0.3311 1.8182 0.8197
MZ7-mel 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.7692 2.9155 0.4329 0.2353 0.2994 0.3448 0.5495 0.6289 2.0080 0.5814 0.6494 0.3003 0.3290 0.4878 0.1996 0.3290 1.6260 0.8333
MZ7-mel 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.9346 2.8986 0.4219 0.2359 0.3247 0.3425 0.6944 0.6410 2.0408 0.6452 0.6579 0.3345 0.3636 0.5650 0.2083 0.3534 0.3906 2.2936 0.7634
MZ7-mel 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.7576 2.3753 0.2227 0.2688 0.2933 0.5587 0.5376 1.8349 0.5650 0.5051 0.2488 0.2801 0.4444 0.2179 0.3311 0.2882 1.4948 0.6944
MZ7-mel 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 1.0776 2.8571 0.2674 0.3571 0.3759 0.7634 2.2124 0.6757 0.6098 0.3521 0.3922 0.5952 0.2092 0.4274 2.0408 0.8130
MZ7-mel 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.6711 1.7762 0.3846 0.2445 0.3040 0.3300 0.5587 0.5208 1.5083 0.4348 0.5102 0.2817 0.4082 0.4000 0.2114 0.2967 0.3165 1.2987 0.8265
MZ7-mel 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.6536 1.7921 0.3690 0.2494 0.2857 0.3165 0.7042 0.5376 1.5408 0.4425 0.4762 0.3185 0.3247 0.4464 0.2088 0.3030 0.3003 1.3831 0.8265
MZ7-mel 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 2.3256
MZ7-mel 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
MZ7-mel 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.7937 2.4331 0.4184 0.2208 0.3077 0.3205 0.5714 0.7143 1.9685 0.5747 0.6579 0.2976 0.3068 0.5102 0.1972 0.3846 0.3546 1.5337 0.8265
MZ7-mel 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.9174 2.6178 0.4785 0.2481 0.3215 0.3497 0.7634 0.6623 2.0534 0.5848 0.6711 0.3185 0.3484 0.5181 0.2008 0.4329 0.3774 1.6556 0.7874
MZ7-mel 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.8000 2.4213 0.3861 0.2232 0.3155 0.3165 0.6173 0.6061 1.9231 0.6250 0.5848 0.3460 0.3484 0.4367 0.2222 0.3846 0.4032 1.5291 0.7634