Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
Download: Excel (XLSX), Comma-separated (CSV)
| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Triciribine | T47D | 1.0 | uM | 6 | hr | 101.59 | 28.35 | p-4EBP1 | 83.39 | |
| Triciribine | T47D | 1.0 | uM | 24 | hr | 164.68 | 77.07 | p-4EBP1 | 336.21 | |
| Dactolisib | T47D | 1.0 | uM | 6 | hr | 88.25 | 32.38 | p-4EBP1 | 5.74 | |
| Dactolisib | T47D | 1.0 | uM | 24 | hr | 116.07 | 42.41 | p-4EBP1 | 9.24 | |
| Gefitinib | AU565 | 3.16 | uM | 6 | hr | 233.77 | 53.87 | p-4EBP1 | 66.06 | |
| Gefitinib | AU565 | 3.16 | uM | 24 | hr | 334.36 | 80.31 | p-4EBP1 | 122.07 | |
| Torkinib | AU565 | 2.37 | uM | 6 | hr | 242.90 | 43.68 | p-4EBP1 | 7.46 | |
| Torkinib | AU565 | 2.37 | uM | 24 | hr | 397.60 | 69.26 | p-4EBP1 | 11.59 | |
| GSK1059615 | AU565 | 3.16 | uM | 6 | hr | 178.44 | 36.96 | p-4EBP1 | 20.50 | |
| GSK1059615 | AU565 | 3.16 | uM | 24 | hr | 375.35 | 78.43 | p-4EBP1 | 38.94 | |
| MK2206 | AU565 | 3.16 | uM | 6 | hr | 395.42 | 73.69 | p-4EBP1 | 128.53 | |
| MK2206 | AU565 | 3.16 | uM | 24 | hr | 337.64 | 74.37 | p-4EBP1 | 110.16 | |
| Triciribine | AU565 | 3.16 | uM | 6 | hr | 254.84 | 61.55 | p-4EBP1 | 62.39 | |
| Triciribine | AU565 | 3.16 | uM | 24 | hr | 430.15 | 83.77 | p-4EBP1 | 111.00 | |
| Dactolisib | AU565 | 3.16 | uM | 6 | hr | 377.06 | 85.43 | p-4EBP1 | 9.63 | |
| Dactolisib | AU565 | 3.16 | uM | 24 | hr | 474.09 | 70.93 | p-4EBP1 | 11.17 | |
| Gefitinib | HCC1954 | 3.16 | uM | 6 | hr | 127.32 | 31.57 | p-4EBP1 | 45.85 | |
| Gefitinib | HCC1954 | 3.16 | uM | 24 | hr | 234.61 | 82.80 | p-4EBP1 | 130.60 | |
| Torkinib | HCC1954 | 2.37 | uM | 6 | hr | 260.07 | 58.14 | p-4EBP1 | 8.55 | |
| Torkinib | HCC1954 | 2.37 | uM | 24 | hr | 233.60 | 73.33 | p-4EBP1 | 14.31 | |
| GSK1059615 | HCC1954 | 3.16 | uM | 6 | hr | 388.17 | 71.55 | p-4EBP1 | 16.94 | |
| GSK1059615 | HCC1954 | 3.16 | uM | 24 | hr | 297.52 | 63.23 | p-4EBP1 | 17.76 | |
| MK2206 | HCC1954 | 3.16 | uM | 6 | hr | 227.07 | 61.78 | p-4EBP1 | 79.73 | |
| MK2206 | HCC1954 | 3.16 | uM | 24 | hr | 255.02 | 78.54 | p-4EBP1 | 99.48 | |
| Triciribine | HCC1954 | 3.16 | uM | 6 | hr | 211.49 | 66.52 | p-4EBP1 | 97.58 | |