Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| GSK1059615 | MCF 10A | 31.6 | nM | 6 | hr | 379.12 | 165.32 | p-4EBP1 | 348.91 | |
| GSK1059615 | MCF 10A | 31.6 | nM | 24 | hr | 290.16 | 157.18 | p-4EBP1 | 233.47 | |
| MK2206 | MCF 10A | 31.6 | nM | 6 | hr | 375.80 | 155.36 | p-4EBP1 | 359.32 | |
| MK2206 | MCF 10A | 31.6 | nM | 24 | hr | 264.50 | 170.32 | p-4EBP1 | 218.34 | |
| Triciribine | MCF 10A | 31.6 | nM | 6 | hr | 379.12 | 156.82 | p-4EBP1 | 360.76 | |
| Triciribine | MCF 10A | 31.6 | nM | 24 | hr | 237.54 | 158.78 | p-4EBP1 | 206.20 | |
| Dactolisib | MCF 10A | 31.6 | nM | 6 | hr | 348.78 | 134.55 | p-4EBP1 | 195.18 | |
| Dactolisib | MCF 10A | 31.6 | nM | 24 | hr | 276.44 | 153.41 | p-4EBP1 | 89.11 | |
| Gefitinib | T47D | 31.6 | nM | 6 | hr | 161.99 | 98.63 | p-4EBP1 | 479.28 | |
| Gefitinib | T47D | 31.6 | nM | 24 | hr | 141.14 | 119.21 | p-4EBP1 | 409.49 | |
| Torkinib | T47D | 7.5 | nM | 6 | hr | 162.62 | 84.08 | p-4EBP1 | 434.94 | |
| Torkinib | T47D | 7.5 | nM | 24 | hr | 135.75 | 78.36 | p-4EBP1 | 306.59 | |
| GSK1059615 | T47D | 31.6 | nM | 6 | hr | 169.81 | 82.41 | p-4EBP1 | 499.15 | |
| GSK1059615 | T47D | 31.6 | nM | 24 | hr | 147.95 | 105.49 | p-4EBP1 | 411.60 | |
| MK2206 | T47D | 31.6 | nM | 6 | hr | 94.55 | 35.69 | p-4EBP1 | 78.93 | |
| MK2206 | T47D | 31.6 | nM | 24 | hr | 148.55 | 96.56 | p-4EBP1 | 352.26 | |
| Triciribine | T47D | 31.6 | nM | 6 | hr | 107.57 | 58.92 | p-4EBP1 | 199.41 | |
| Triciribine | T47D | 31.6 | nM | 24 | hr | 160.29 | 105.55 | p-4EBP1 | 367.55 | |
| Dactolisib | T47D | 31.6 | nM | 6 | hr | 90.45 | 41.37 | p-4EBP1 | 104.60 | |
| Dactolisib | T47D | 31.6 | nM | 24 | hr | 112.85 | 54.74 | p-4EBP1 | 222.74 | |
| Gefitinib | AU565 | 100.0 | nM | 6 | hr | 277.70 | 67.19 | p-4EBP1 | 80.51 | |
| Gefitinib | AU565 | 100.0 | nM | 24 | hr | 469.54 | 116.93 | p-4EBP1 | 140.88 | |
| Torkinib | AU565 | 31.6 | nM | 6 | hr | 251.01 | 49.47 | p-4EBP1 | 31.81 | |
| Torkinib | AU565 | 31.6 | nM | 24 | hr | 395.95 | 75.66 | p-4EBP1 | 84.49 | |
| GSK1059615 | AU565 | 100.0 | nM | 6 | hr | 260.75 | 64.41 | p-4EBP1 | 85.16 | |