Responses of breast cancer lines to growth factors
Analysis of growth factor signaling in genetically diverse breast cancer lines
Mario Niepel1*, Marc Hafner1*, Emily A. Pace2*, Mirra Chung1, Diana H. Chai2, Lili Zhou1, Jeremy L. Muhlich1, Birgit Schoeberl2 and Peter K. Sorger1
1HMS LINCS Center; 2Merrimack Pharmaceuticals
Growth factor signaling is a key determinant of cellular behavior in normal and diseased tissue. Here we determined the variance of signaling responses across a panel of breast cancer lines and identified potential molecular underpinnings of these responses. We correlated basal receptors levels with ligand responses to derive a simple heuristic that approximates the responsiveness of cells to individual growth factors.
- We analyzed the response of 39 breast cancer cell lines to 15 ligands at 2 doses, 3 time points by measuring pERK and pAKT levels and we related these responses to clinical cancer subtypes and the expression and phosphorylation levels of RTKs.
- The response to ErbB ligands is ubiquitous across virtually all cell lines and many respond to HGF, FGFs, and IGF/INS.
- TNBCs are highly responsive to a variety of ligands, while HER2amp cell lines only show muted responses.
- The responsiveness of individual cell lines can be determined by a simple heuristic.
- We published an interactive website to browse the data by cell lines and ligands.
|1.||Results of kinetic clustering and dose-dependence classification||Details||Download (.xlsx)|
|2.||All ligand responses (Data from Sci. Signal., 2013, Vol. 6, ra84)||Details||Download (.xls)|
|3.||All basal levels (Data from Sci. Signal., 2013, Vol. 6, ra84)||Details||Download (.xls)|