Results of kinetic clustering and dose-dependence classification ================================================================ Kinetic clustering ------------------ We used clustering to determine which characteristics of immediate-early signaling are most variable across our dataset, and whether they correlate with cell line, ligand identity, or receptor family. Unsupervised k-means clustering (with k=4) of the time-series data identified trajectories that correspond to sustained, transient, late, or no response Dose-dependence classification ------------------------------ We classified each treatment into one of five groups based on how the pERK and pAKT responses differed at high and low ligand doses. The classification groups are as follows: equal response; stronger response at 100ng than 1ng; response only at 100ng; no response; response only at 1ng.