Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Iniparib 0.3160 0.1067
uM
 48
hr
 1 1399 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 304.34 NGFR rabbit IgG(H+L) / donkey NGFR 159.18
Colo858-B2-rep1
B3
COLO 858 Vemurafenib CTB 0.3160 0.1067
uM
 48
hr
 1 1399 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 278.66 NGFR rabbit IgG(H+L) / donkey NGFR 181.20
Colo858-B2-rep1
B4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1405 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 151.46 NGFR rabbit IgG(H+L) / donkey NGFR 1114.77
WM115-B2-rep1
A11
WM-115 Vemurafenib Rucaparib 1.0000 0.5333
uM
 48
hr
 2 1408 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 147.49 NGFR rabbit IgG(H+L) / donkey NGFR 1104.73
WM115-B1-rep2
C3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1409 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.89 NGFR rabbit IgG(H+L) / donkey NGFR 1192.22
WM115-B2-rep1
A10
WM-115 Vemurafenib Azacitidine 1.0000 0.1067
uM
 48
hr
 2 1410 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.24 NGFR rabbit IgG(H+L) / donkey NGFR 1072.40
WM115-B2-rep2
E2
WM-115 Vemurafenib UNC1215 1.0000 0.5333
uM
 48
hr
 1 1417 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.34 NGFR rabbit IgG(H+L) / donkey NGFR 1116.16
WM115-B1-rep1
C8
WM-115 Vemurafenib Pracinostat 1.0000 0.5333
uM
 48
hr
 2 1417 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.20 NGFR rabbit IgG(H+L) / donkey NGFR 609.07
WM115-B1-rep2
F4
COLO 858 Vemurafenib Ischemin 0.3160 0.1067
uM
 48
hr
 1 1417 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 326.68 NGFR rabbit IgG(H+L) / donkey NGFR 144.77
Colo858-B2-rep1
G7
WM-115 Vemurafenib GSK-J2 1.0000 0.1067
uM
 48
hr
 2 1417 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.53 NGFR rabbit IgG(H+L) / donkey NGFR 1067.45
WM115-B2-rep2
C6
WM-115 Vemurafenib IOX2 1.0000 2.6667
uM
 48
hr
 1 1418 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.29 NGFR rabbit IgG(H+L) / donkey NGFR 964.73
WM115-A2-rep1
D8
WM-115 Vemurafenib Vorinostat 1.0000 2.6667
uM
 48
hr
 2 1419 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.82 NGFR rabbit IgG(H+L) / donkey NGFR 531.22
WM115-A2-rep2
D2
WM-115 Vemurafenib Daminozide 1.0000 0.5333
uM
 48
hr
 2 1419 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.10 NGFR rabbit IgG(H+L) / donkey NGFR 1163.35
WM115-B1-rep2
E6
WM-115 Vemurafenib AGK2 1.0000 0.1067
uM
 48
hr
 1 1420 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.59 NGFR rabbit IgG(H+L) / donkey NGFR 1264.14
WM115-B2-rep1
D5
WM-115 Vemurafenib Iniparib 1.0000 0.1067
uM
 48
hr
 1 1420 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 147.78 NGFR rabbit IgG(H+L) / donkey NGFR 1106.46
WM115-B2-rep1
B3
WM-115 Vemurafenib GSK-J2 1.0000 0.5333
uM
 48
hr
 1 1420 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.29 NGFR rabbit IgG(H+L) / donkey NGFR 997.01
WM115-B1-rep1
C6
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1421 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 197.45 NGFR rabbit IgG(H+L) / donkey NGFR 159.25
A375-B1-rep2
H3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1422 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 173.16 NGFR rabbit IgG(H+L) / donkey NGFR 882.89
WM115-A2-rep2
A2
WM-115 Vemurafenib CTB 1.0000 0.1067
uM
 48
hr
 1 1423 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 146.93 NGFR rabbit IgG(H+L) / donkey NGFR 1068.64
WM115-B2-rep1
B4
WM-115 Vemurafenib Pracinostat 1.0000 0.1067
uM
 48
hr
 1 1425 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.48 NGFR rabbit IgG(H+L) / donkey NGFR 911.70
WM115-B2-rep1
F4
WM-115 Vemurafenib I-BET 1.0000 0.1067
uM
 48
hr
 2 1425 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.30 NGFR rabbit IgG(H+L) / donkey NGFR 924.32
WM115-B2-rep2
E7
WM-115 Vemurafenib Methylstat 1.0000 0.1067
uM
 48
hr
 2 1428 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.16 NGFR rabbit IgG(H+L) / donkey NGFR 1139.78
WM115-B2-rep2
F6
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1429 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 185.39 NGFR rabbit IgG(H+L) / donkey NGFR 144.00
A375-B1-rep2
H10
COLO 858 Vemurafenib Olaparib 0.3160 2.6667
uM
 48
hr
 2 1429 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 244.86 NGFR rabbit IgG(H+L) / donkey NGFR 158.62
Colo858-A2-rep2
B2
WM-115 Vemurafenib CTB 1.0000 0.5333
uM
 48
hr
 1 1430 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.37 NGFR rabbit IgG(H+L) / donkey NGFR 993.65
WM115-B1-rep1
B4