Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Epigallocatechin gallate 1.0000 2.6667
uM
 48
hr
 1 1303 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.63 NGFR rabbit IgG(H+L) / donkey NGFR 1092.09
WM115-A2-rep1
E8
WM-115 Vemurafenib Pracinostat 1.0000 2.6667
uM
 48
hr
 1 784 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 114.81 NGFR rabbit IgG(H+L) / donkey NGFR 370.58
WM115-A2-rep1
F4
WM-115 Vemurafenib BIX-01294 1.0000 2.6667
uM
 48
hr
 1 985 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 241.84 NGFR rabbit IgG(H+L) / donkey NGFR 563.90
WM115-A2-rep1
F5
WM-115 Vemurafenib I-BET151 1.0000 2.6667
uM
 48
hr
 1 691 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.59 NGFR rabbit IgG(H+L) / donkey NGFR 497.01
WM115-A2-rep1
F7
WM-115 Vemurafenib CX-5461 1.0000 2.6667
uM
 48
hr
 1 1088 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.79 NGFR rabbit IgG(H+L) / donkey NGFR 1051.81
WM115-A2-rep1
F8
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1093 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.58 NGFR rabbit IgG(H+L) / donkey NGFR 1088.13
WM115-A2-rep1
A10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1094 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 146.45 NGFR rabbit IgG(H+L) / donkey NGFR 1030.60
WM115-A2-rep1
A11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1054 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.53 NGFR rabbit IgG(H+L) / donkey NGFR 926.14
WM115-A2-rep1
A3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1158 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.15 NGFR rabbit IgG(H+L) / donkey NGFR 1015.40
WM115-A2-rep1
A4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 930 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.08 NGFR rabbit IgG(H+L) / donkey NGFR 1077.20
WM115-A2-rep1
A7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 997 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.02 NGFR rabbit IgG(H+L) / donkey NGFR 955.42
WM115-A2-rep1
A8
WM-115 Vemurafenib Olaparib 1.0000 2.6667
uM
 48
hr
 1 1273 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.00 NGFR rabbit IgG(H+L) / donkey NGFR 997.40
WM115-A2-rep1
B2
WM-115 Vemurafenib Iniparib 1.0000 2.6667
uM
 48
hr
 1 1294 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.06 NGFR rabbit IgG(H+L) / donkey NGFR 1014.90
WM115-A2-rep1
B3
WM-115 Vemurafenib PFI-1 1.0000 2.6667
uM
 48
hr
 1 1044 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.16 NGFR rabbit IgG(H+L) / donkey NGFR 698.55
WM115-A2-rep1
B7
WM-115 Vemurafenib UNC669 1.0000 2.6667
uM
 48
hr
 1 1162 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.02 NGFR rabbit IgG(H+L) / donkey NGFR 1039.91
WM115-A2-rep1
B8
WM-115 Vemurafenib Rucaparib 1.0000 2.6667
uM
 48
hr
 1 1345 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 148.87 NGFR rabbit IgG(H+L) / donkey NGFR 1031.56
WM115-A2-rep1
C3
WM-115 Vemurafenib Belinostat 1.0000 2.6667
uM
 48
hr
 1 705 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.59 NGFR rabbit IgG(H+L) / donkey NGFR 342.51
WM115-A2-rep1
C4
WM-115 Vemurafenib (+)-JQ1 1.0000 2.6667
uM
 48
hr
 1 521 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 121.85 NGFR rabbit IgG(H+L) / donkey NGFR 452.89
WM115-A2-rep1
C7
WM-115 Vemurafenib UNC1215 1.0000 2.6667
uM
 48
hr
 1 1451 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.72 NGFR rabbit IgG(H+L) / donkey NGFR 1069.96
WM115-A2-rep1
C8
WM-115 Vemurafenib JW55 1.0000 2.6667
uM
 48
hr
 1 1394 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.07 NGFR rabbit IgG(H+L) / donkey NGFR 1001.10
WM115-A2-rep1
D3
WM-115 Vemurafenib Entinostat 1.0000 2.6667
uM
 48
hr
 1 1496 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 148.91 NGFR rabbit IgG(H+L) / donkey NGFR 594.32
WM115-A2-rep1
D4
WM-115 Vemurafenib Azacitidine 1.0000 2.6667
uM
 48
hr
 1 589 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 180.96 NGFR rabbit IgG(H+L) / donkey NGFR 483.03
WM115-A2-rep1
E2
WM-115 Vemurafenib C646 1.0000 2.6667
uM
 48
hr
 1 829 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.27 NGFR rabbit IgG(H+L) / donkey NGFR 903.05
WM115-A2-rep1
E3
WM-115 Vemurafenib Tranylcypromine 1.0000 2.6667
uM
 48
hr
 1 1043 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.10 NGFR rabbit IgG(H+L) / donkey NGFR 964.47
WM115-A2-rep1
G6
WM-115 Vemurafenib UNC0638 1.0000 2.6667
uM
 48
hr
 1 1121 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 170.32 NGFR rabbit IgG(H+L) / donkey NGFR 760.80
WM115-A2-rep1
G5