Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Rucaparib 1.0000 2.6667
uM
 48
hr
 1 1345 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 148.87 NGFR rabbit IgG(H+L) / donkey NGFR 1031.56
WM115-A2-rep1
C3
WM-115 Vemurafenib Belinostat 1.0000 2.6667
uM
 48
hr
 1 705 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.59 NGFR rabbit IgG(H+L) / donkey NGFR 342.51
WM115-A2-rep1
C4
WM-115 Vemurafenib Selisistat 1.0000 2.6667
uM
 48
hr
 1 1455 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.38 NGFR rabbit IgG(H+L) / donkey NGFR 1061.79
WM115-A2-rep1
C5
WM-115 Vemurafenib GSK-J2 1.0000 2.6667
uM
 48
hr
 1 1446 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.27 NGFR rabbit IgG(H+L) / donkey NGFR 1076.83
WM115-A2-rep1
C6
WM-115 Vemurafenib (+)-JQ1 1.0000 2.6667
uM
 48
hr
 1 521 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 121.85 NGFR rabbit IgG(H+L) / donkey NGFR 452.89
WM115-A2-rep1
C7
WM-115 Vemurafenib UNC1215 1.0000 2.6667
uM
 48
hr
 1 1451 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.72 NGFR rabbit IgG(H+L) / donkey NGFR 1069.96
WM115-A2-rep1
C8
WM-115 Vemurafenib Vorinostat 1.0000 2.6667
uM
 48
hr
 1 1431 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.45 NGFR rabbit IgG(H+L) / donkey NGFR 436.29
WM115-A2-rep1
D2
WM-115 Vemurafenib JW55 1.0000 2.6667
uM
 48
hr
 1 1394 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.07 NGFR rabbit IgG(H+L) / donkey NGFR 1001.10
WM115-A2-rep1
D3
WM-115 Vemurafenib Entinostat 1.0000 2.6667
uM
 48
hr
 1 1496 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 148.91 NGFR rabbit IgG(H+L) / donkey NGFR 594.32
WM115-A2-rep1
D4
WM-115 Vemurafenib AGK2 1.0000 2.6667
uM
 48
hr
 1 193 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.10 NGFR rabbit IgG(H+L) / donkey NGFR 876.79
WM115-A2-rep1
D5
WM-115 Vemurafenib GSK-J4 1.0000 2.6667
uM
 48
hr
 1 1461 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.76 NGFR rabbit IgG(H+L) / donkey NGFR 1082.05
WM115-A2-rep1
D6
WM-115 Vemurafenib (-)-JQ1 1.0000 2.6667
uM
 48
hr
 1 1468 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.58 NGFR rabbit IgG(H+L) / donkey NGFR 994.87
WM115-A2-rep1
D7
WM-115 Vemurafenib IOX2 1.0000 2.6667
uM
 48
hr
 1 1418 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.29 NGFR rabbit IgG(H+L) / donkey NGFR 964.73
WM115-A2-rep1
D8
WM-115 Vemurafenib Azacitidine 1.0000 2.6667
uM
 48
hr
 1 589 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 180.96 NGFR rabbit IgG(H+L) / donkey NGFR 483.03
WM115-A2-rep1
E2
WM-115 Vemurafenib C646 1.0000 2.6667
uM
 48
hr
 1 829 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.27 NGFR rabbit IgG(H+L) / donkey NGFR 903.05
WM115-A2-rep1
E3
WM-115 Vemurafenib Mocetinostat 1.0000 2.6667
uM
 48
hr
 1 987 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.34 NGFR rabbit IgG(H+L) / donkey NGFR 568.17
WM115-A2-rep1
E4
WM-115 Vemurafenib Resveratrol 1.0000 2.6667
uM
 48
hr
 1 1297 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.13 NGFR rabbit IgG(H+L) / donkey NGFR 1028.89
WM115-A2-rep1
E5
WM-115 Vemurafenib Daminozide 1.0000 2.6667
uM
 48
hr
 1 1297 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.03 NGFR rabbit IgG(H+L) / donkey NGFR 1069.08
WM115-A2-rep1
E6
WM-115 Vemurafenib I-BET 1.0000 2.6667
uM
 48
hr
 1 1020 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.67 NGFR rabbit IgG(H+L) / donkey NGFR 541.61
WM115-A2-rep1
E7
WM-115 Vemurafenib Epigallocatechin gallate 1.0000 2.6667
uM
 48
hr
 1 1303 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.63 NGFR rabbit IgG(H+L) / donkey NGFR 1092.09
WM115-A2-rep1
E8
WM-115 Vemurafenib Decitabine 1.0000 2.6667
uM
 48
hr
 1 1175 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.60 NGFR rabbit IgG(H+L) / donkey NGFR 898.23
WM115-A2-rep1
F2
WM-115 Vemurafenib Garcinol 1.0000 2.6667
uM
 48
hr
 1 758 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.60 NGFR rabbit IgG(H+L) / donkey NGFR 1025.88
WM115-A2-rep1
F3
WM-115 Vemurafenib Pracinostat 1.0000 2.6667
uM
 48
hr
 1 784 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 114.81 NGFR rabbit IgG(H+L) / donkey NGFR 370.58
WM115-A2-rep1
F4
WM-115 Vemurafenib BIX-01294 1.0000 2.6667
uM
 48
hr
 1 985 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 241.84 NGFR rabbit IgG(H+L) / donkey NGFR 563.90
WM115-A2-rep1
F5
WM-115 Vemurafenib Methylstat 1.0000 2.6667
uM
 48
hr
 1 908 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 117.67 NGFR rabbit IgG(H+L) / donkey NGFR 816.15
WM115-A2-rep1
F6