Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib UNC1215 0.3160 2.6667
uM
 48
hr
 2 2480 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 325.51 NGFR rabbit IgG(H+L) / donkey NGFR 148.28
Colo858-A2-rep2
C8
COLO 858 Vemurafenib Vorinostat 0.3160 2.6667
uM
 48
hr
 2 478 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 242.50 NGFR rabbit IgG(H+L) / donkey NGFR 28.47
Colo858-A2-rep2
D2
COLO 858 Vemurafenib JW55 0.3160 2.6667
uM
 48
hr
 2 1836 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 220.99 NGFR rabbit IgG(H+L) / donkey NGFR 102.31
Colo858-A2-rep2
D3
COLO 858 Vemurafenib Entinostat 0.3160 2.6667
uM
 48
hr
 2 2000 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.13 NGFR rabbit IgG(H+L) / donkey NGFR 12.84
Colo858-A2-rep2
D4
COLO 858 Vemurafenib AGK2 0.3160 2.6667
uM
 48
hr
 2 635 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 266.45 NGFR rabbit IgG(H+L) / donkey NGFR 71.47
Colo858-A2-rep2
D5
COLO 858 Vemurafenib GSK-J4 0.3160 2.6667
uM
 48
hr
 2 682 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 189.00 NGFR rabbit IgG(H+L) / donkey NGFR 11.65
Colo858-A2-rep2
D6
COLO 858 Vemurafenib (-)-JQ1 0.3160 2.6667
uM
 48
hr
 2 3101 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 297.94 NGFR rabbit IgG(H+L) / donkey NGFR 134.38
Colo858-A2-rep2
D7
COLO 858 Vemurafenib IOX2 0.3160 2.6667
uM
 48
hr
 2 3207 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 355.43 NGFR rabbit IgG(H+L) / donkey NGFR 154.65
Colo858-A2-rep2
D8
COLO 858 Vemurafenib Azacitidine 0.3160 2.6667
uM
 48
hr
 2 674 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 228.99 NGFR rabbit IgG(H+L) / donkey NGFR 27.17
Colo858-A2-rep2
E2
COLO 858 Vemurafenib C646 0.3160 2.6667
uM
 48
hr
 2 2543 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.83 NGFR rabbit IgG(H+L) / donkey NGFR 167.50
Colo858-A2-rep2
E3
COLO 858 Vemurafenib Mocetinostat 0.3160 2.6667
uM
 48
hr
 2 2331 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 165.13 NGFR rabbit IgG(H+L) / donkey NGFR 22.79
Colo858-A2-rep2
E4
COLO 858 Vemurafenib Resveratrol 0.3160 2.6667
uM
 48
hr
 2 2640 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.76 NGFR rabbit IgG(H+L) / donkey NGFR 122.85
Colo858-A2-rep2
E5
COLO 858 Vemurafenib Daminozide 0.3160 2.6667
uM
 48
hr
 2 3110 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 319.78 NGFR rabbit IgG(H+L) / donkey NGFR 135.02
Colo858-A2-rep2
E6
COLO 858 Vemurafenib I-BET 0.3160 2.6667
uM
 48
hr
 2 2077 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.89 NGFR rabbit IgG(H+L) / donkey NGFR 10.47
Colo858-A2-rep2
E7
COLO 858 Vemurafenib Epigallocatechin gallate 0.3160 2.6667
uM
 48
hr
 2 2922 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 321.58 NGFR rabbit IgG(H+L) / donkey NGFR 130.77
Colo858-A2-rep2
E8
COLO 858 Vemurafenib Decitabine 0.3160 2.6667
uM
 48
hr
 2 1525 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 271.76 NGFR rabbit IgG(H+L) / donkey NGFR 135.82
Colo858-A2-rep2
F2
COLO 858 Vemurafenib Garcinol 0.3160 2.6667
uM
 48
hr
 2 1133 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 279.50 NGFR rabbit IgG(H+L) / donkey NGFR 140.01
Colo858-A2-rep2
F3
COLO 858 Vemurafenib Pracinostat 0.3160 2.6667
uM
 48
hr
 2 127 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 200.69 NGFR rabbit IgG(H+L) / donkey NGFR 37.13
Colo858-A2-rep2
F4
COLO 858 Vemurafenib BIX-01294 0.3160 2.6667
uM
 48
hr
 2 721 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.48 NGFR rabbit IgG(H+L) / donkey NGFR 30.74
Colo858-A2-rep2
F5
COLO 858 Vemurafenib Methylstat 0.3160 2.6667
uM
 48
hr
 2 1461 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.86 NGFR rabbit IgG(H+L) / donkey NGFR 69.08
Colo858-A2-rep2
F6
COLO 858 Vemurafenib I-BET151 0.3160 2.6667
uM
 48
hr
 2 1438 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 110.13 NGFR rabbit IgG(H+L) / donkey NGFR 13.48
Colo858-A2-rep2
F7
COLO 858 Vemurafenib CX-5461 0.3160 2.6667
uM
 48
hr
 2 1927 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 203.59 NGFR rabbit IgG(H+L) / donkey NGFR 217.19
Colo858-A2-rep2
F8
COLO 858 Vemurafenib RG-108 0.3160 2.6667
uM
 48
hr
 2 1341 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 308.36 NGFR rabbit IgG(H+L) / donkey NGFR 140.74
Colo858-A2-rep2
G2
COLO 858 Vemurafenib Anacardic acid 0.3160 2.6667
uM
 48
hr
 2 998 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 267.99 NGFR rabbit IgG(H+L) / donkey NGFR 163.66
Colo858-A2-rep2
G3
COLO 858 Vemurafenib MC1568 0.3160 2.6667
uM
 48
hr
 2 1124 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.62 NGFR rabbit IgG(H+L) / donkey NGFR 110.02
Colo858-A2-rep2
G4