Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Triciribine | T47D | 0 (control) | 24 | hr | 156.16 | 103.29 | p-4EBP1 | 393.33 | | |
| Dactolisib | T47D | 0 (control) | 6 | hr | 102.03 | 45.53 | p-4EBP1 | 121.29 | | |
| Dactolisib | T47D | 0 (control) | 24 | hr | 136.31 | 100.86 | p-4EBP1 | 345.05 | | |
| Gefitinib | AU565 | 1.0 | nM | 6 | hr | 289.01 | 63.74 | p-4EBP1 | 68.42 | |
| Gefitinib | AU565 | 1.0 | nM | 24 | hr | 597.04 | 145.45 | p-4EBP1 | 170.31 | |
| Torkinib | AU565 | 0.1 | nM | 6 | hr | 346.86 | 73.14 | p-4EBP1 | 98.91 | |
| Torkinib | AU565 | 0.1 | nM | 24 | hr | 484.96 | 127.02 | p-4EBP1 | 215.15 | |
| GSK1059615 | AU565 | 1.0 | nM | 6 | hr | 261.27 | 65.61 | p-4EBP1 | 75.05 | |
| GSK1059615 | AU565 | 1.0 | nM | 24 | hr | 424.14 | 125.45 | p-4EBP1 | 174.34 | |
| MK2206 | AU565 | 1.0 | nM | 6 | hr | 273.47 | 71.35 | p-4EBP1 | 96.49 | |
| MK2206 | AU565 | 1.0 | nM | 24 | hr | 416.57 | 131.35 | p-4EBP1 | 192.89 | |
| Triciribine | AU565 | 1.0 | nM | 6 | hr | 291.94 | 77.13 | p-4EBP1 | 98.69 | |
| Triciribine | AU565 | 1.0 | nM | 24 | hr | 492.56 | 131.78 | p-4EBP1 | 221.65 | |
| Dactolisib | AU565 | 1.0 | nM | 6 | hr | 202.47 | 47.00 | p-4EBP1 | 51.99 | |
| Dactolisib | AU565 | 1.0 | nM | 24 | hr | 513.37 | 123.17 | p-4EBP1 | 183.62 | |
| Gefitinib | HCC1954 | 1.0 | nM | 6 | hr | 192.39 | 39.79 | p-4EBP1 | 52.60 | |
| Gefitinib | HCC1954 | 1.0 | nM | 24 | hr | 286.14 | 129.34 | p-4EBP1 | 149.15 | |
| Torkinib | HCC1954 | 0.1 | nM | 6 | hr | 181.17 | 40.64 | p-4EBP1 | 53.94 | |
| Torkinib | HCC1954 | 0.1 | nM | 24 | hr | 268.67 | 120.63 | p-4EBP1 | 124.48 | |
| GSK1059615 | HCC1954 | 1.0 | nM | 6 | hr | 373.59 | 94.92 | p-4EBP1 | 112.38 | |
| GSK1059615 | HCC1954 | 1.0 | nM | 24 | hr | 310.46 | 118.73 | p-4EBP1 | 119.18 | |
| MK2206 | HCC1954 | 1.0 | nM | 6 | hr | 194.30 | 65.17 | p-4EBP1 | 85.41 | |
| MK2206 | HCC1954 | 1.0 | nM | 24 | hr | 287.93 | 122.38 | p-4EBP1 | 149.60 | |
| Triciribine | HCC1954 | 1.0 | nM | 6 | hr | 235.54 | 85.78 | p-4EBP1 | 104.82 | |
| Triciribine | HCC1954 | 1.0 | nM | 24 | hr | 303.58 | 117.35 | p-4EBP1 | 135.97 | |