184A1 F3aN400-Venus-P2A-NLS-mCherry - Cell Line (ID:51129)
HMS LINCS ID: | 51129 |
Name: | 184A1 F3aN400-Venus-P2A-NLS-mCherry |
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LINCS ID: | |
Alternative ID: | CLO_0001137 |
Parent Cell Line: | HMSL51088-3: 184A1 |
Reference Source: | Somponnat Sampattavanich (Mahidol University) |
Organism: | Homo sapiens |
Organ: | breast |
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Production Details: | Modification type: stable piggyBac transposon-mediated transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated construct: pPB-CAG.EBNXN (A. Bradley, Sanger Institute) containing FoxO3aN400-HA-Venus-P2A-NLS-Myc-mCherry, which includes a fragment of human FoxO3a spanning residues 1-400 and carrying a loss-of-function H212R mutation in the DNA binding domain (PMID: 11964479) |
Genetic Modification(s): | pPB-CAG-FoxO3aN400-HA-Venus-P2A-NLS-Myc-mCherry |
Known Mutations: | |
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Growth Properties: | adherent |
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Date Publicly Available: | 2017-05-02 |
Most Recent Update: | 2017-05-02 |
Datasets:
HMS Dataset ID | Dataset Title | HMS Dataset Type |
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20290 | Growth factor-induced FoxO3a nuclear-cytoplasmic pulsing dynamics of non-phosphorylatable reporters in mammary epithelial cell lines. Dataset 1 of 3: single-cell reporter measurements. | Microscopy/Imaging |
20291 | Growth factor-induced FoxO3a nuclear-cytoplasmic pulsing dynamics of non-phosphorylatable reporters in mammary epithelial cell lines. Dataset 2 of 3: single-cell reporter pulsing metrics. | Analysis |
20292 | Growth factor-induced FoxO3a nuclear-cytoplasmic pulsing dynamics of non-phosphorylatable reporters in mammary epithelial cell lines. Dataset 3 of 3: mean reporter pulsing metrics. | Analysis |
20293 | IGF-I-induced FoxO3a nuclear-cytoplasmic pulsing dynamics in mammary epithelial cell lines following treatment with increasing doses of an AKT inhibitor. Dataset 1 of 3: single-cell reporter measurements. | Microscopy/Imaging |
20294 | IGF-I-induced FoxO3a nuclear-cytoplasmic pulsing dynamics in mammary epithelial cell lines following treatment with increasing doses of an AKT inhibitor. Dataset 2 of 3: single-cell reporter pulsing metrics. | Analysis |
20295 | IGF-I-induced FoxO3a nuclear-cytoplasmic pulsing dynamics in mammary epithelial cell lines following treatment with increasing doses of an AKT inhibitor. Dataset 3 of 3: mean reporter pulsing metrics. | Analysis |
20296 | EGF-induced FoxO3a nuclear-cytoplasmic pulsing dynamics in mammary epithelial cell lines following treatment with increasing doses of a MEK inhibitor. Dataset 1 of 3: single-cell reporter measurements. | Microscopy/Imaging |
20297 | EGF-induced FoxO3a nuclear-cytoplasmic pulsing dynamics in mammary epithelial cell lines following treatment with increasing doses of a MEK inhibitor. Dataset 2 of 3: single-cell reporter pulsing metrics. | Analysis |
20298 | EGF-induced FoxO3a nuclear-cytoplasmic pulsing dynamics in mammary epithelial cell lines following treatment with increasing doses of a MEK inhibitor. Dataset 3 of 3: mean reporter pulsing metrics. | Analysis |
Batch Information:
HMS LINCS Batch ID | Provider | Provider Catalog ID | Provider Batch ID |
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51129-1 | Somponnat Sampattavanich (Mahidol University) |