RNAseq:
RNAseq_Assay_Name: 3' DGEseq
RNAseq_Assay_Date: 9/1/2017
RNAseq_Instrument: NextSeq
RNAseq_Software_Version:
RNAseq_Single_Paired: Paired End
RNAseq_Read_Length_1: 75
RNAseq_Read_Length_2: 75
RNAseq_Strand_Specificity: Stranded
RNAseq_Library_starting_quantity: 55 ng
RNAseq_RNA_Integrity_Number: 9
RNAseq_Lysis_method: TCL lysis buffer + 1% beta-mercaptoethanol
RNAseq_Extraction_method: SPRI beads
RNAseq_Fragmentation_method: transposon-based
RNAseq_Size_selection_method: SPRI beads
RNAseq_Size_Range: 200-500
RNAseq_Spike-in_Controls: PhiX
RNAseq_Spike-in_Source: Illumina
RNAseq_Spike-in_Concentration: ~12%
RNAseq_Mean_Sequencing_Depth: ~230000
RNAseq_Sequencing_Cycles: 75 paired end
RNAseq_Reference_Genome: GRCh38
RNAseq_Trim_metric: <30 (Phred)
RNAseq_Lib_prep_kit: Nextera DNA kit
RNAseq_Biosample_Type: cell lines
RNAseq_Biosample_summary: human breast cell lines
RNAseq_Associated_SOPs: Nextera DNA kit
RNAseq_Replication_Type: triplicate
RNAseq_Replication_Count: 3
RNAseq_Library_Prep_Date: 8/24/2017
RNAseq_Library_Date_Extracted: 8/11/2017
RNAseq_Spike-in_Time: Spike-in added to library prior to sequencing
RNAseq_Library_Indexing_Basepairing: 8
RNAseq_Barcode: N701
RNAseq_cDNA_Preparation_Method: Random
RNAseq_PCR_Cycle_Number: 5
RNAseq_Sequencing_Facility: Harvard University Bauer core
RNAseq_Sequencing_Chemistry_Version: v2
RNAseq_Raw_File_Type: fastq
RNAseq_Raw_File_Replicate_Number:
RNAseq_Plate_Format: 336
RNAseq_Temperature:
RNAseq_Complexity_Perturbagen: Multiple
RNAseq_Complexity_Concentration: Multiple
RNAseq_Complexity_Cell_Type: Multiple
RNAseq_Complexity_Cell_Density: Multiple
RNAseq_Complexity_Time_Point: One
RNAseq_Complexity_Perturbagen_Number: No
RNAseq_Concentration: n/a
RNAseq_Time_Point: 6 h