DGE-seq Gene expression data for 7 breast cancer cell lines treated with abemaciclib, palbociclib, ribociclib at 0.1, 0.3, 1, or 3 μM, or alvocidib at 0.1 or 1 μM for 6 hours - Dataset (ID:20373)

    
  RNAseq: 
    RNAseq_Assay_Name: 3' DGEseq 
    RNAseq_Assay_Date: 9/1/2017 
    RNAseq_Instrument: NextSeq 
    RNAseq_Software_Version:  
    RNAseq_Single_Paired: Paired End 
    RNAseq_Read_Length_1: 75 
    RNAseq_Read_Length_2: 75 
    RNAseq_Strand_Specificity: Stranded 
    RNAseq_Library_starting_quantity: 55 ng 
    RNAseq_RNA_Integrity_Number: 9 
    RNAseq_Lysis_method: TCL lysis buffer + 1% beta-mercaptoethanol 
    RNAseq_Extraction_method: SPRI beads 
    RNAseq_Fragmentation_method: transposon-based 
    RNAseq_Size_selection_method: SPRI beads 
    RNAseq_Size_Range: 200-500 
    RNAseq_Spike-in_Controls: PhiX 
    RNAseq_Spike-in_Source: Illumina 
    RNAseq_Spike-in_Concentration: ~12% 
    RNAseq_Mean_Sequencing_Depth: ~230000 
    RNAseq_Sequencing_Cycles: 75 paired end 
    RNAseq_Reference_Genome: GRCh38 
    RNAseq_Trim_metric: <30 (Phred) 
    RNAseq_Lib_prep_kit: Nextera DNA kit 
    RNAseq_Biosample_Type: cell lines 
    RNAseq_Biosample_summary: human breast cell lines 
    RNAseq_Associated_SOPs: Nextera DNA kit 
    RNAseq_Replication_Type: triplicate 
    RNAseq_Replication_Count: 3 
    RNAseq_Library_Prep_Date: 8/24/2017 
    RNAseq_Library_Date_Extracted: 8/11/2017 
    RNAseq_Spike-in_Time: Spike-in added to library prior to sequencing 
    RNAseq_Library_Indexing_Basepairing: 8 
    RNAseq_Barcode: N701 
    RNAseq_cDNA_Preparation_Method: Random 
    RNAseq_PCR_Cycle_Number: 5 
    RNAseq_Sequencing_Facility: Harvard University Bauer core 
    RNAseq_Sequencing_Chemistry_Version: v2 
    RNAseq_Raw_File_Type: fastq 
    RNAseq_Raw_File_Replicate_Number:  
    RNAseq_Plate_Format: 336 
    RNAseq_Temperature:  
    RNAseq_Complexity_Perturbagen: Multiple 
    RNAseq_Complexity_Concentration: Multiple 
    RNAseq_Complexity_Cell_Type: Multiple 
    RNAseq_Complexity_Cell_Density: Multiple 
    RNAseq_Complexity_Time_Point: One 
    RNAseq_Complexity_Perturbagen_Number: No 
    RNAseq_Concentration: n/a 
    RNAseq_Time_Point: 6 h