Breast Cancer Profiling Project – Proteomics 3: 1 total proteome dataset for a 27-cell line breast cancer panel under basal conditions - Dataset (ID:20370)

    
  PROTEOMICS: 
    PROTEOMICS_Assay_Name: DDA 
    PROTEOMICS_Assay_Type: TMT 
    PROTEOMICS_SamplePrep_Lysis_Buffer: SDS (2% SDS 150 mM NaCl 50 mM Tris pH8.5 1X HALT 2 mM sodium vanadate) 
    PROTEOMICS_SamplePrep_Lysis_Protocol: NA 
    PROTEOMICS_SamplePrep_Subcellular_Fractionation: FALSE 
    PROTEOMICS_SamplePrep_Subcellular_Fractionation_Target: NA 
    PROTEOMICS_SamplePrep_Protein_Reduction_Agent: DTT 
    PROTEOMICS_SamplePrep_Alkylation_Agent: IAA 
    PROTEOMICS_SamplePrep_Protein_Enrichment: FALSE 
    PROTEOMICS_SamplePrep_Protein_Enrichment_Target: NA 
    PROTEOMICS_SamplePrep_Protein_Fractionation: FALSE 
    PROTEOMICS_SamplePrep_Protein_Fractionation_mode: NA 
    PROTEOMICS_SamplePrep_Peptide_Enrichment: FALSE 
    PROTEOMICS_SamplePrep_Peptide_Enrichment_Target: NA 
    PROTEOMICS_SamplePrep_Peptide_Fractionation: TRUE 
    PROTEOMICS_SamplePrep_Peptide_Fractionation_mode: alkaline reverse phase 
    PROTEOMICS_SamplePrep_Automation: FALSE 
    PROTEOMICS_SamplePrep_Digestion_Enzyme: Trypsin and LysC mix 
    PROTEOMICS_SamplePrep_Digestion_Standard: FALSE 
    PROTEOMICS_SamplePrep_Digestion_Standard_AA_sequence: NA 
    PROTEOMICS_MS_Instrument: Orbitrap Fusion Lumos MS 
    PROTEOMICS_MS_Instrument_Software: Xcalibur 
    PROTEOMICS_MS_Instrument_Software_Version: 3.3x 
    PROTEOMICS_MS_Instrument_Sample_Interface: LC 
    PROTEOMICS_Processing_Primary_Signal_Processing: Xcalibur 
    PROTEOMICS_Processing_Primary_Signal_Processing_Version: FASTA_DATABASE 
    PROTEOMICS_Processing_Analyte_Detection_Mode: Sequest 
    PROTEOMICS_Processing_Analyte_Detection_FILE: NA 
    PROTEOMICS_Processing_Static_Modification_AA(+/-amu): Carbamidomethy C(+57.021454) TMT N-terminus TMT on K 
    PROTEOMICS_Processing_Differential_ModificationAA(+/-amu): oxidation M+15.994915 
    PROTEOMICS_Processing_Statistical_Processing: github.com/datarail/msda 
    PROTEOMICS_Processing_Statistical_Processing_Version: NA