PROTEOMICS:
PROTEOMICS_Assay_Name: DDA
PROTEOMICS_Assay_Type: TMT
PROTEOMICS_SamplePrep_Lysis_Buffer: SDS (2% SDS 150 mM NaCl 50 mM Tris pH8.5 1X HALT 2 mM sodium vanadate)
PROTEOMICS_SamplePrep_Lysis_Protocol: NA
PROTEOMICS_SamplePrep_Subcellular_Fractionation: FALSE
PROTEOMICS_SamplePrep_Subcellular_Fractionation_Target: NA
PROTEOMICS_SamplePrep_Protein_Reduction_Agent: DTT
PROTEOMICS_SamplePrep_Alkylation_Agent: IAA
PROTEOMICS_SamplePrep_Protein_Enrichment: FALSE
PROTEOMICS_SamplePrep_Protein_Enrichment_Target: NA
PROTEOMICS_SamplePrep_Protein_Fractionation: FALSE
PROTEOMICS_SamplePrep_Protein_Fractionation_mode: NA
PROTEOMICS_SamplePrep_Peptide_Enrichment: FALSE
PROTEOMICS_SamplePrep_Peptide_Enrichment_Target: NA
PROTEOMICS_SamplePrep_Peptide_Fractionation: TRUE
PROTEOMICS_SamplePrep_Peptide_Fractionation_mode: alkaline reverse phase
PROTEOMICS_SamplePrep_Automation: FALSE
PROTEOMICS_SamplePrep_Digestion_Enzyme: Trypsin and LysC mix
PROTEOMICS_SamplePrep_Digestion_Standard: FALSE
PROTEOMICS_SamplePrep_Digestion_Standard_AA_sequence: NA
PROTEOMICS_MS_Instrument: Orbitrap Fusion Lumos MS
PROTEOMICS_MS_Instrument_Software: Xcalibur
PROTEOMICS_MS_Instrument_Software_Version: 3.3x
PROTEOMICS_MS_Instrument_Sample_Interface: LC
PROTEOMICS_Processing_Primary_Signal_Processing: Xcalibur
PROTEOMICS_Processing_Primary_Signal_Processing_Version: FASTA_DATABASE
PROTEOMICS_Processing_Analyte_Detection_Mode: Sequest
PROTEOMICS_Processing_Analyte_Detection_FILE: NA
PROTEOMICS_Processing_Static_Modification_AA(+/-amu): Carbamidomethy C(+57.021454) TMT N-terminus TMT on K
PROTEOMICS_Processing_Differential_ModificationAA(+/-amu): oxidation M+15.994915
PROTEOMICS_Processing_Statistical_Processing: github.com/datarail/msda
PROTEOMICS_Processing_Statistical_Processing_Version: NA