LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 3 of 6: Cytosolic fluorescent intensity measurements at 48 hr. - Dataset (ID:20305)
- Detail
- Small Molecules Studied
- Cell Lines Studied
- Proteins Studied
- Antibodies Studied
- Other Reagents Studied
- Results
Antibodies Studied
Download: Excel (XLSX), Comma-separated (CSV)
- 21 Antibodies
| HMS LINCS ID | Name | Alternative Names | Clone Name | LINCS ID | Resource Identification Initiative ID | Nominal Protein Target HMS LINCS ID(s) |
|---|---|---|---|---|---|---|
| 80001-1 | H3 (pS10) | Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #3475 | D2C8 | AB_10694639 | ||
| 80002-2 | p21 / Alexa Fluor 647 | p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor® 647 Conjugate) #8587 | 12D1 | AB_10892861 | ||
| 80003-1 | KI-67 / clone D3B5 | Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) #11882 | D3B5 | |||
| 80004-1 | Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555 | Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #8957 | D20B12 | |||
| 80005-1 | S6 (pS235; pS236) / Alexa Fluor 647 | Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #4851 | D57.2.2E | AB_916160 | ||
| 80008-1 | EGFR | EGF Receptor (D38B1) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #5616 | D38B1 | AB_10691853 | ||
| 80011-1 | PCNA | PCNA (PC10) Mouse mAb (Alexa Fluor® 488 Conjugate) #8580 | PC10 | AB_11178664 | ||
| 80012-1 | ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) / Alexa Fluor 488 | Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #4344 | D13.14.4E | AB_2139960 | ||
| 80022-1 | p53 | p53 Antibody (DO-1): sc-126 | DO-1 | AB_628082 | ||
| 80024-2 | mouse IgG(H+L) / goat | Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 647 conjugate | AB_10564125 | |||
| 80029-1 | p27Kip1 / clone Y236 | Anti-p27 KIP 1 antibody [Y236] (Alexa Fluor® 647) (ab194234) | Y236 | |||
| 80035-1 | FOXO3a | FoxO3a (75D8) Rabbit mAb #2497 | 75D8 | AB_836876 | ||
| 80036-1 | Cyclin-D1 | Anti-Cyclin D1 antibody [EPR2241] (Alexa Fluor® 488) (ab190194) | EPR2241 | |||
| 80082-1 | RP-A p32 | RPA32/RPA2 (4E4) Rat mAb #2208 | 4E4 | AB_2238543 | ||
| 80083-1 | E2F-1 | Anti-E2F1 antibody [EPR3818(3)] (Alexa Fluor® 555) (ab208078) | EPR3818(3) | |||
| 80084-1 | FOXO1a | Anti-FOXO1A antibody [EP927Y] (Alexa Fluor® 555) (ab207244) | EP927Y | |||
| 80085-1 | c-Myc | Anti-c-Myc antibody [Y69] (Alexa Fluor® 555) (ab201780) | Y69 | |||
| 80086-1 | H2A.X (pS139) / clone 2F3 | Alexa Fluor® 647 anti-H2A.X Phospho (Ser139) Antibody | ||||
| 80087-1 | Cyclin-E1 / eFluor 660 | Anti-Human Cyclin E eFluor® 660 | HE12 | AB_2574350 | ||
| 80088-1 | rat IgG(H+L) | Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 | AB_2535855 | |||
| 80089-1 | rabbit IgG(H+L) / goat | Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | AB_2576217 |
- 21 Antibodies