LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 3 of 6: Cytosolic fluorescent intensity measurements at 48 hr. - Dataset (ID:20305)

Antibodies Studied

  • 21 Antibodies
HMS LINCS ID Name Alternative Names Clone Name LINCS ID Resource Identification Initiative ID Nominal Protein Target HMS LINCS ID(s)
80001-1
H3 (pS10)
Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #3475
D2C8 AB_10694639
80002-2
p21 / Alexa Fluor 647
p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor® 647 Conjugate) #8587
12D1 AB_10892861
80003-1
KI-67 / clone D3B5
Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) #11882
D3B5
80004-1
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555
Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #8957
D20B12
80005-1
S6 (pS235; pS236) / Alexa Fluor 647
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #4851
D57.2.2E AB_916160
80008-1
EGFR
EGF Receptor (D38B1) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #5616
D38B1 AB_10691853
80011-1
PCNA
PCNA (PC10) Mouse mAb (Alexa Fluor® 488 Conjugate) #8580
PC10 AB_11178664
80012-1
ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) / Alexa Fluor 488
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #4344
D13.14.4E AB_2139960
80022-1
p53
p53 Antibody (DO-1): sc-126
DO-1 AB_628082
80024-2
mouse IgG(H+L) / goat
Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 647 conjugate
AB_10564125
80029-1
p27Kip1 / clone Y236
Anti-p27 KIP 1 antibody [Y236] (Alexa Fluor® 647) (ab194234)
Y236
80035-1
FOXO3a
FoxO3a (75D8) Rabbit mAb #2497
75D8 AB_836876
80036-1
Cyclin-D1
Anti-Cyclin D1 antibody [EPR2241] (Alexa Fluor® 488) (ab190194)
EPR2241
80082-1
RP-A p32
RPA32/RPA2 (4E4) Rat mAb #2208
4E4 AB_2238543
80083-1
E2F-1
Anti-E2F1 antibody [EPR3818(3)] (Alexa Fluor® 555) (ab208078)
EPR3818(3)
80084-1
FOXO1a
Anti-FOXO1A antibody [EP927Y] (Alexa Fluor® 555) (ab207244)
EP927Y
80085-1
c-Myc
Anti-c-Myc antibody [Y69] (Alexa Fluor® 555) (ab201780)
Y69
80086-1
H2A.X (pS139) / clone 2F3
Alexa Fluor® 647 anti-H2A.X Phospho (Ser139) Antibody
80087-1
Cyclin-E1 / eFluor 660
Anti-Human Cyclin E eFluor® 660
HE12 AB_2574350
80088-1
rat IgG(H+L)
Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555
AB_2535855
80089-1
rabbit IgG(H+L) / goat
Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488
AB_2576217
  • 21 Antibodies