Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib (-)-JQ1 0.3160 0.1067
uM
 48
hr
 2 3368 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 384.19 NGFR rabbit IgG(H+L) / donkey NGFR 168.62
Colo858-B2-rep2
D7
COLO 858 Vemurafenib IOX2 0.3160 0.1067
uM
 48
hr
 2 3195 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 403.34 NGFR rabbit IgG(H+L) / donkey NGFR 186.02
Colo858-B2-rep2
D8
COLO 858 Vemurafenib Azacitidine 0.3160 0.1067
uM
 48
hr
 2 2234 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 274.09 NGFR rabbit IgG(H+L) / donkey NGFR 137.68
Colo858-B2-rep2
E2
COLO 858 Vemurafenib C646 0.3160 0.1067
uM
 48
hr
 2 2646 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 378.38 NGFR rabbit IgG(H+L) / donkey NGFR 139.85
Colo858-B2-rep2
E3
COLO 858 Vemurafenib Mocetinostat 0.3160 0.1067
uM
 48
hr
 2 3092 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 219.79 NGFR rabbit IgG(H+L) / donkey NGFR 83.00
Colo858-B2-rep2
E4
COLO 858 Vemurafenib Resveratrol 0.3160 0.1067
uM
 48
hr
 2 3137 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 346.17 NGFR rabbit IgG(H+L) / donkey NGFR 157.63
Colo858-B2-rep2
E5
COLO 858 Vemurafenib Daminozide 0.3160 0.1067
uM
 48
hr
 2 3171 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 314.34 NGFR rabbit IgG(H+L) / donkey NGFR 161.15
Colo858-B2-rep2
E6
COLO 858 Vemurafenib I-BET 0.3160 0.1067
uM
 48
hr
 2 2556 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 250.08 NGFR rabbit IgG(H+L) / donkey NGFR 76.21
Colo858-B2-rep2
E7
COLO 858 Vemurafenib Epigallocatechin gallate 0.3160 0.1067
uM
 48
hr
 2 3297 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 322.94 NGFR rabbit IgG(H+L) / donkey NGFR 169.39
Colo858-B2-rep2
E8
COLO 858 Vemurafenib Decitabine 0.3160 0.1067
uM
 48
hr
 2 1989 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 259.19 NGFR rabbit IgG(H+L) / donkey NGFR 178.70
Colo858-B2-rep2
F2
COLO 858 Vemurafenib Garcinol 0.3160 0.1067
uM
 48
hr
 2 2520 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 363.75 NGFR rabbit IgG(H+L) / donkey NGFR 149.22
Colo858-B2-rep2
F3
COLO 858 Vemurafenib Pracinostat 0.3160 0.1067
uM
 48
hr
 2 1001 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.71 NGFR rabbit IgG(H+L) / donkey NGFR 138.38
Colo858-B2-rep2
F4
COLO 858 Vemurafenib BIX-01294 0.3160 0.1067
uM
 48
hr
 2 2596 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 356.20 NGFR rabbit IgG(H+L) / donkey NGFR 139.03
Colo858-B2-rep2
F5
COLO 858 Vemurafenib Methylstat 0.3160 0.1067
uM
 48
hr
 2 2596 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 315.46 NGFR rabbit IgG(H+L) / donkey NGFR 165.29
Colo858-B2-rep2
F6
COLO 858 Vemurafenib I-BET151 0.3160 0.1067
uM
 48
hr
 2 2368 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 194.16 NGFR rabbit IgG(H+L) / donkey NGFR 42.29
Colo858-B2-rep2
F7
COLO 858 Vemurafenib CX-5461 0.3160 0.1067
uM
 48
hr
 2 2469 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 222.16 NGFR rabbit IgG(H+L) / donkey NGFR 215.24
Colo858-B2-rep2
F8
COLO 858 Vemurafenib RG-108 0.3160 0.1067
uM
 48
hr
 2 1903 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 295.84 NGFR rabbit IgG(H+L) / donkey NGFR 163.68
Colo858-B2-rep2
G2
COLO 858 Vemurafenib Anacardic acid 0.3160 0.1067
uM
 48
hr
 2 1570 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 344.62 NGFR rabbit IgG(H+L) / donkey NGFR 122.24
Colo858-B2-rep2
G3
COLO 858 Vemurafenib MC1568 0.3160 0.1067
uM
 48
hr
 2 1738 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 316.29 NGFR rabbit IgG(H+L) / donkey NGFR 132.37
Colo858-B2-rep2
G4
COLO 858 Vemurafenib UNC0638 0.3160 0.1067
uM
 48
hr
 2 1720 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 295.24 NGFR rabbit IgG(H+L) / donkey NGFR 192.89
Colo858-B2-rep2
G5
COLO 858 Vemurafenib Tranylcypromine 0.3160 0.1067
uM
 48
hr
 2 1549 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 334.43 NGFR rabbit IgG(H+L) / donkey NGFR 145.32
Colo858-B2-rep2
G6
COLO 858 Vemurafenib Ischemin 0.3160 0.1067
uM
 48
hr
 2 1683 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 304.71 NGFR rabbit IgG(H+L) / donkey NGFR 137.29
Colo858-B2-rep2
G7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1333 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 323.49 NGFR rabbit IgG(H+L) / donkey NGFR 141.87
Colo858-B2-rep2
H10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1012 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 248.16 NGFR rabbit IgG(H+L) / donkey NGFR 135.16
Colo858-B2-rep2
H11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1281 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 283.24 NGFR rabbit IgG(H+L) / donkey NGFR 140.21
Colo858-B2-rep2
H2