Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib UNC0638 0.3160 2.6667
uM
 48
hr
 2 793 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 188.46 NGFR rabbit IgG(H+L) / donkey NGFR 153.42
Colo858-A2-rep2
G5
COLO 858 Vemurafenib Tranylcypromine 0.3160 2.6667
uM
 48
hr
 2 1329 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 304.95 NGFR rabbit IgG(H+L) / donkey NGFR 179.43
Colo858-A2-rep2
G6
COLO 858 Vemurafenib Ischemin 0.3160 2.6667
uM
 48
hr
 2 1515 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 335.23 NGFR rabbit IgG(H+L) / donkey NGFR 124.34
Colo858-A2-rep2
G7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 901 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 241.75 NGFR rabbit IgG(H+L) / donkey NGFR 128.46
Colo858-A2-rep2
H10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 865 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 215.59 NGFR rabbit IgG(H+L) / donkey NGFR 149.37
Colo858-A2-rep2
H11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 992 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 322.85 NGFR rabbit IgG(H+L) / donkey NGFR 126.77
Colo858-A2-rep2
H2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 920 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 277.58 NGFR rabbit IgG(H+L) / donkey NGFR 140.83
Colo858-A2-rep2
H3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 728 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 275.60 NGFR rabbit IgG(H+L) / donkey NGFR 129.02
Colo858-A2-rep2
H4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 818 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 270.37 NGFR rabbit IgG(H+L) / donkey NGFR 132.19
Colo858-A2-rep2
H5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 951 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 247.15 NGFR rabbit IgG(H+L) / donkey NGFR 133.80
Colo858-A2-rep2
H6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 903 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 227.64 NGFR rabbit IgG(H+L) / donkey NGFR 138.37
Colo858-A2-rep2
H7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 922 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 278.87 NGFR rabbit IgG(H+L) / donkey NGFR 126.84
Colo858-A2-rep2
H8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 780 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 234.33 NGFR rabbit IgG(H+L) / donkey NGFR 134.94
Colo858-A2-rep2
H9
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1121 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 328.09 NGFR rabbit IgG(H+L) / donkey NGFR 131.17
Colo858-B1-rep1
A10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1350 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 353.28 NGFR rabbit IgG(H+L) / donkey NGFR 136.44
Colo858-B1-rep1
A11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1184 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 361.53 NGFR rabbit IgG(H+L) / donkey NGFR 140.40
Colo858-B1-rep1
A2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 887 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 344.64 NGFR rabbit IgG(H+L) / donkey NGFR 133.90
Colo858-B1-rep1
A3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1214 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 380.70 NGFR rabbit IgG(H+L) / donkey NGFR 139.87
Colo858-B1-rep1
A4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1008 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 371.10 NGFR rabbit IgG(H+L) / donkey NGFR 120.59
Colo858-B1-rep1
A5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1005 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 348.84 NGFR rabbit IgG(H+L) / donkey NGFR 143.10
Colo858-B1-rep1
A6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1082 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 358.68 NGFR rabbit IgG(H+L) / donkey NGFR 117.87
Colo858-B1-rep1
A7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 970 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 376.63 NGFR rabbit IgG(H+L) / donkey NGFR 125.61
Colo858-B1-rep1
A8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1228 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 378.70 NGFR rabbit IgG(H+L) / donkey NGFR 125.18
Colo858-B1-rep1
A9
COLO 858 Vemurafenib Olaparib 0.3160 0.5333
uM
 48
hr
 1 1838 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 311.35 NGFR rabbit IgG(H+L) / donkey NGFR 143.80
Colo858-B1-rep1
B2
COLO 858 Vemurafenib Iniparib 0.3160 0.5333
uM
 48
hr
 1 1621 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 314.44 NGFR rabbit IgG(H+L) / donkey NGFR 151.86
Colo858-B1-rep1
B3