Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1442 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.89 NGFR rabbit IgG(H+L) / donkey NGFR 1114.51
WM115-B2-rep1
A9
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1350 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 187.39 NGFR rabbit IgG(H+L) / donkey NGFR 169.71
A375-A2-rep2
H2
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1289 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 205.41 NGFR rabbit IgG(H+L) / donkey NGFR 165.25
A375-A2-rep2
H11
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1358 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 192.78 NGFR rabbit IgG(H+L) / donkey NGFR 135.20
A375-A2-rep2
H10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1144 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.31 NGFR rabbit IgG(H+L) / donkey NGFR 1008.74
WM115-B2-rep2
H8
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2409 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 178.97 NGFR rabbit IgG(H+L) / donkey NGFR 199.27
A375-A2-rep2
A9
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1871 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 184.59 NGFR rabbit IgG(H+L) / donkey NGFR 210.13
A375-A2-rep2
A8
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1797 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 197.36 NGFR rabbit IgG(H+L) / donkey NGFR 224.46
A375-A2-rep2
A7
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2033 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 217.52 NGFR rabbit IgG(H+L) / donkey NGFR 238.98
A375-A2-rep2
A6
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2194 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 194.15 NGFR rabbit IgG(H+L) / donkey NGFR 186.08
A375-A2-rep2
A5
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2355 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 209.47 NGFR rabbit IgG(H+L) / donkey NGFR 230.86
A375-A2-rep2
A4
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2177 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 175.69 NGFR rabbit IgG(H+L) / donkey NGFR 218.26
A375-A2-rep2
A3
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2412 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 231.59 NGFR rabbit IgG(H+L) / donkey NGFR 226.51
A375-A2-rep2
A2
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1793 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.73 NGFR rabbit IgG(H+L) / donkey NGFR 197.31
A375-A2-rep2
A11
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1915 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 204.90 NGFR rabbit IgG(H+L) / donkey NGFR 199.36
A375-A2-rep2
A10
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1087 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.27 NGFR rabbit IgG(H+L) / donkey NGFR 138.42
A375-A2-rep1
H9
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1099 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.81 NGFR rabbit IgG(H+L) / donkey NGFR 136.50
A375-A2-rep1
H8
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1074 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.31 NGFR rabbit IgG(H+L) / donkey NGFR 161.00
A375-A2-rep1
H7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 883 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.08 NGFR rabbit IgG(H+L) / donkey NGFR 1102.94
WM115-B2-rep1
H10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 952 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.09 NGFR rabbit IgG(H+L) / donkey NGFR 915.79
WM115-B2-rep1
H11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 939 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.31 NGFR rabbit IgG(H+L) / donkey NGFR 946.36
WM115-B2-rep1
H2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 845 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.87 NGFR rabbit IgG(H+L) / donkey NGFR 1057.39
WM115-B2-rep1
H3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 808 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.62 NGFR rabbit IgG(H+L) / donkey NGFR 1052.15
WM115-B2-rep1
H4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 746 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.54 NGFR rabbit IgG(H+L) / donkey NGFR 1007.03
WM115-B2-rep1
H5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 904 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.03 NGFR rabbit IgG(H+L) / donkey NGFR 939.97
WM115-B2-rep1
H6