Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Olaparib 0.3160 0.5333
uM
 48
hr
 2 1658 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 296.94 NGFR rabbit IgG(H+L) / donkey NGFR 158.73
Colo858-B1-rep2
B2
A-375 Vemurafenib Olaparib 1.0000 2.6667
uM
 48
hr
 2 2229 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.21 NGFR rabbit IgG(H+L) / donkey NGFR 210.77
A375-A2-rep2
B2
A-375 Vemurafenib Olaparib 1.0000 0.5333
uM
 48
hr
 1 2160 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.98 NGFR rabbit IgG(H+L) / donkey NGFR 170.41
A375-B1-rep1
B2
WM-115 Vemurafenib Olaparib 1.0000 0.1067
uM
 48
hr
 2 1291 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.34 NGFR rabbit IgG(H+L) / donkey NGFR 1162.67
WM115-B2-rep2
B2
COLO 858 Vemurafenib PFI-1 0.3160 0.5333
uM
 48
hr
 1 1513 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 278.22 NGFR rabbit IgG(H+L) / donkey NGFR 84.85
Colo858-B1-rep1
B7
WM-115 Vemurafenib PFI-1 1.0000 2.6667
uM
 48
hr
 1 1044 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.16 NGFR rabbit IgG(H+L) / donkey NGFR 698.55
WM115-A2-rep1
B7
COLO 858 Vemurafenib PFI-1 0.3160 0.1067
uM
 48
hr
 2 2090 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 400.42 NGFR rabbit IgG(H+L) / donkey NGFR 145.17
Colo858-B2-rep2
B7
COLO 858 Vemurafenib PFI-1 0.3160 2.6667
uM
 48
hr
 2 1014 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.85 NGFR rabbit IgG(H+L) / donkey NGFR 19.30
Colo858-A2-rep2
B7
WM-115 Vemurafenib PFI-1 1.0000 0.5333
uM
 48
hr
 1 1121 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 128.12 NGFR rabbit IgG(H+L) / donkey NGFR 999.98
WM115-B1-rep1
B7
A-375 Vemurafenib PFI-1 1.0000 0.5333
uM
 48
hr
 2 2340 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.42 NGFR rabbit IgG(H+L) / donkey NGFR 204.08
A375-B1-rep2
B7
COLO 858 Vemurafenib PFI-1 0.3160 2.6667
uM
 48
hr
 1 1383 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.34 NGFR rabbit IgG(H+L) / donkey NGFR 30.41
Colo858-A2-rep1
B7
COLO 858 Vemurafenib PFI-1 0.3160 0.1067
uM
 48
hr
 1 1597 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 305.58 NGFR rabbit IgG(H+L) / donkey NGFR 152.98
Colo858-B2-rep1
B7
WM-115 Vemurafenib PFI-1 1.0000 0.5333
uM
 48
hr
 2 1302 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.78 NGFR rabbit IgG(H+L) / donkey NGFR 1035.27
WM115-B1-rep2
B7
A-375 Vemurafenib PFI-1 1.0000 0.1067
uM
 48
hr
 2 2198 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.45 NGFR rabbit IgG(H+L) / donkey NGFR 167.49
A375-B2-rep2
B7
WM-115 Vemurafenib PFI-1 1.0000 2.6667
uM
 48
hr
 2 1357 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.24 NGFR rabbit IgG(H+L) / donkey NGFR 628.79
WM115-A2-rep2
B7
A-375 Vemurafenib PFI-1 1.0000 2.6667
uM
 48
hr
 2 2464 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.44 NGFR rabbit IgG(H+L) / donkey NGFR 154.43
A375-A2-rep2
B7
COLO 858 Vemurafenib PFI-1 0.3160 0.5333
uM
 48
hr
 2 1454 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 310.50 NGFR rabbit IgG(H+L) / donkey NGFR 102.50
Colo858-B1-rep2
B7
A-375 Vemurafenib PFI-1 1.0000 0.5333
uM
 48
hr
 1 2521 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.70 NGFR rabbit IgG(H+L) / donkey NGFR 191.24
A375-B1-rep1
B7
WM-115 Vemurafenib PFI-1 1.0000 0.1067
uM
 48
hr
 2 1186 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.71 NGFR rabbit IgG(H+L) / donkey NGFR 1098.16
WM115-B2-rep2
B7
WM-115 Vemurafenib PFI-1 1.0000 0.1067
uM
 48
hr
 1 1493 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.99 NGFR rabbit IgG(H+L) / donkey NGFR 1089.78
WM115-B2-rep1
B7
A-375 Vemurafenib PFI-1 1.0000 2.6667
uM
 48
hr
 1 2255 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.05 NGFR rabbit IgG(H+L) / donkey NGFR 162.18
A375-A2-rep1
B7
A-375 Vemurafenib PFI-1 1.0000 0.1067
uM
 48
hr
 1 2527 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 190.19 NGFR rabbit IgG(H+L) / donkey NGFR 218.09
A375-B2-rep1
B7
WM-115 Vemurafenib Pracinostat 1.0000 0.5333
uM
 48
hr
 1 1333 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.90 NGFR rabbit IgG(H+L) / donkey NGFR 666.71
WM115-B1-rep1
F4
COLO 858 Vemurafenib Pracinostat 0.3160 0.1067
uM
 48
hr
 2 1001 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.71 NGFR rabbit IgG(H+L) / donkey NGFR 138.38
Colo858-B2-rep2
F4
COLO 858 Vemurafenib Pracinostat 0.3160 2.6667
uM
 48
hr
 2 127 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 200.69 NGFR rabbit IgG(H+L) / donkey NGFR 37.13
Colo858-A2-rep2
F4