Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Anacardic acid 1.0000 2.6667
uM
 48
hr
 1 1318 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.31 NGFR rabbit IgG(H+L) / donkey NGFR 170.13
A375-A2-rep1
G3
WM-115 Vemurafenib Anacardic acid 1.0000 2.6667
uM
 48
hr
 2 1013 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 132.50 NGFR rabbit IgG(H+L) / donkey NGFR 989.81
WM115-A2-rep2
G3
WM-115 Vemurafenib Anacardic acid 1.0000 2.6667
uM
 48
hr
 1 997 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.62 NGFR rabbit IgG(H+L) / donkey NGFR 928.34
WM115-A2-rep1
G3
WM-115 Vemurafenib Anacardic acid 1.0000 0.1067
uM
 48
hr
 1 1092 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.00 NGFR rabbit IgG(H+L) / donkey NGFR 967.15
WM115-B2-rep1
G3
COLO 858 Vemurafenib Anacardic acid 0.3160 0.5333
uM
 48
hr
 2 926 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 272.48 NGFR rabbit IgG(H+L) / donkey NGFR 124.30
Colo858-B1-rep2
G3
COLO 858 Vemurafenib Anacardic acid 0.3160 2.6667
uM
 48
hr
 1 1253 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 315.28 NGFR rabbit IgG(H+L) / donkey NGFR 120.19
Colo858-A2-rep1
G3
COLO 858 Vemurafenib Anacardic acid 0.3160 2.6667
uM
 48
hr
 2 998 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 267.99 NGFR rabbit IgG(H+L) / donkey NGFR 163.66
Colo858-A2-rep2
G3
COLO 858 Vemurafenib Anacardic acid 0.3160 0.5333
uM
 48
hr
 1 1751 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 322.89 NGFR rabbit IgG(H+L) / donkey NGFR 153.60
Colo858-B1-rep1
G3
COLO 858 Vemurafenib Anacardic acid 0.3160 0.1067
uM
 48
hr
 1 1144 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 317.68 NGFR rabbit IgG(H+L) / donkey NGFR 153.35
Colo858-B2-rep1
G3
COLO 858 Vemurafenib Anacardic acid 0.3160 0.1067
uM
 48
hr
 2 1570 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 344.62 NGFR rabbit IgG(H+L) / donkey NGFR 122.24
Colo858-B2-rep2
G3
WM-115 Vemurafenib Anacardic acid 1.0000 0.1067
uM
 48
hr
 2 1318 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.59 NGFR rabbit IgG(H+L) / donkey NGFR 1134.58
WM115-B2-rep2
G3
A-375 Vemurafenib Azacitidine 1.0000 2.6667
uM
 48
hr
 2 1303 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 286.33 NGFR rabbit IgG(H+L) / donkey NGFR 62.73
A375-A2-rep2
E2
A-375 Vemurafenib Azacitidine 1.0000 0.1067
uM
 48
hr
 1 2464 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 196.92 NGFR rabbit IgG(H+L) / donkey NGFR 145.59
A375-B2-rep1
E2
COLO 858 Vemurafenib Azacitidine 0.3160 2.6667
uM
 48
hr
 2 674 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 228.99 NGFR rabbit IgG(H+L) / donkey NGFR 27.17
Colo858-A2-rep2
E2
WM-115 Vemurafenib Azacitidine 1.0000 0.1067
uM
 48
hr
 1 1371 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.64 NGFR rabbit IgG(H+L) / donkey NGFR 985.52
WM115-B2-rep1
E2
WM-115 Vemurafenib Azacitidine 1.0000 0.5333
uM
 48
hr
 2 1168 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.86 NGFR rabbit IgG(H+L) / donkey NGFR 911.40
WM115-B1-rep2
E2
A-375 Vemurafenib Azacitidine 1.0000 0.5333
uM
 48
hr
 1 1856 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 191.48 NGFR rabbit IgG(H+L) / donkey NGFR 101.73
A375-B1-rep1
E2
WM-115 Vemurafenib Azacitidine 1.0000 2.6667
uM
 48
hr
 2 841 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.83 NGFR rabbit IgG(H+L) / donkey NGFR 592.72
WM115-A2-rep2
E2
A-375 Vemurafenib Azacitidine 1.0000 0.1067
uM
 48
hr
 2 2202 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 173.55 NGFR rabbit IgG(H+L) / donkey NGFR 137.51
A375-B2-rep2
E2
COLO 858 Vemurafenib Azacitidine 0.3160 0.5333
uM
 48
hr
 1 1775 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 290.70 NGFR rabbit IgG(H+L) / donkey NGFR 98.06
Colo858-B1-rep1
E2
A-375 Vemurafenib Azacitidine 1.0000 0.5333
uM
 48
hr
 2 2016 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 219.91 NGFR rabbit IgG(H+L) / donkey NGFR 107.93
A375-B1-rep2
E2
COLO 858 Vemurafenib Azacitidine 0.3160 0.1067
uM
 48
hr
 1 1758 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 254.56 NGFR rabbit IgG(H+L) / donkey NGFR 128.93
Colo858-B2-rep1
E2
COLO 858 Vemurafenib Azacitidine 0.3160 0.5333
uM
 48
hr
 2 1199 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 281.80 NGFR rabbit IgG(H+L) / donkey NGFR 95.43
Colo858-B1-rep2
E2
COLO 858 Vemurafenib Azacitidine 0.3160 0.1067
uM
 48
hr
 2 2234 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 274.09 NGFR rabbit IgG(H+L) / donkey NGFR 137.68
Colo858-B2-rep2
E2
A-375 Vemurafenib Azacitidine 1.0000 2.6667
uM
 48
hr
 1 1210 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 261.75 NGFR rabbit IgG(H+L) / donkey NGFR 53.65
A375-A2-rep1
E2