Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib GSK-J4 1.0000 2.6667
uM
 48
hr
 1 1461 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.76 NGFR rabbit IgG(H+L) / donkey NGFR 1082.05
WM115-A2-rep1
D6
COLO 858 Vemurafenib GSK-J4 0.3160 0.5333
uM
 48
hr
 1 2928 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 284.13 NGFR rabbit IgG(H+L) / donkey NGFR 124.77
Colo858-B1-rep1
D6
WM-115 Vemurafenib GSK-J4 1.0000 2.6667
uM
 48
hr
 2 1545 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.21 NGFR rabbit IgG(H+L) / donkey NGFR 1027.05
WM115-A2-rep2
D6
A-375 Vemurafenib GSK-J4 1.0000 0.5333
uM
 48
hr
 2 3446 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.35 NGFR rabbit IgG(H+L) / donkey NGFR 187.41
A375-B1-rep2
D6
COLO 858 Vemurafenib GSK-J4 0.3160 2.6667
uM
 48
hr
 2 682 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 189.00 NGFR rabbit IgG(H+L) / donkey NGFR 11.65
Colo858-A2-rep2
D6
A-375 Vemurafenib GSK-J4 1.0000 2.6667
uM
 48
hr
 2 1738 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 131.40 NGFR rabbit IgG(H+L) / donkey NGFR 104.43
A375-A2-rep2
D6
WM-115 Vemurafenib GSK-J4 1.0000 0.5333
uM
 48
hr
 1 1681 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.56 NGFR rabbit IgG(H+L) / donkey NGFR 1168.38
WM115-B1-rep1
D6
WM-115 Vemurafenib GSK-J4 1.0000 0.1067
uM
 48
hr
 2 1662 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.28 NGFR rabbit IgG(H+L) / donkey NGFR 1193.23
WM115-B2-rep2
D6
COLO 858 Vemurafenib GSK-J4 0.3160 0.5333
uM
 48
hr
 2 3044 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 288.92 NGFR rabbit IgG(H+L) / donkey NGFR 131.62
Colo858-B1-rep2
D6
COLO 858 Vemurafenib GSK-J4 0.3160 0.1067
uM
 48
hr
 2 3387 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 368.22 NGFR rabbit IgG(H+L) / donkey NGFR 158.47
Colo858-B2-rep2
D6
A-375 Vemurafenib GSK-J4 1.0000 0.1067
uM
 48
hr
 1 3386 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 161.57 NGFR rabbit IgG(H+L) / donkey NGFR 228.63
A375-B2-rep1
D6
COLO 858 Vemurafenib GSK-J4 0.3160 0.1067
uM
 48
hr
 1 3271 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 334.17 NGFR rabbit IgG(H+L) / donkey NGFR 153.69
Colo858-B2-rep1
D6
A-375 Vemurafenib GSK-J4 1.0000 0.1067
uM
 48
hr
 2 3245 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 160.22 NGFR rabbit IgG(H+L) / donkey NGFR 162.74
A375-B2-rep2
D6
WM-115 Vemurafenib I-BET 1.0000 2.6667
uM
 48
hr
 2 1147 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 121.03 NGFR rabbit IgG(H+L) / donkey NGFR 490.84
WM115-A2-rep2
E7
COLO 858 Vemurafenib I-BET 0.3160 2.6667
uM
 48
hr
 1 2153 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.18 NGFR rabbit IgG(H+L) / donkey NGFR 12.87
Colo858-A2-rep1
E7
WM-115 Vemurafenib I-BET 1.0000 0.5333
uM
 48
hr
 1 1388 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 131.94 NGFR rabbit IgG(H+L) / donkey NGFR 759.27
WM115-B1-rep1
E7
COLO 858 Vemurafenib I-BET 0.3160 2.6667
uM
 48
hr
 2 2077 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.89 NGFR rabbit IgG(H+L) / donkey NGFR 10.47
Colo858-A2-rep2
E7
A-375 Vemurafenib I-BET 1.0000 0.5333
uM
 48
hr
 2 2710 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.52 NGFR rabbit IgG(H+L) / donkey NGFR 139.02
A375-B1-rep2
E7
COLO 858 Vemurafenib I-BET 0.3160 0.5333
uM
 48
hr
 2 2224 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 161.45 NGFR rabbit IgG(H+L) / donkey NGFR 24.73
Colo858-B1-rep2
E7
COLO 858 Vemurafenib I-BET 0.3160 0.1067
uM
 48
hr
 1 2640 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 228.39 NGFR rabbit IgG(H+L) / donkey NGFR 75.66
Colo858-B2-rep1
E7
A-375 Vemurafenib I-BET 1.0000 2.6667
uM
 48
hr
 2 2493 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.30 NGFR rabbit IgG(H+L) / donkey NGFR 93.88
A375-A2-rep2
E7
A-375 Vemurafenib I-BET 1.0000 0.1067
uM
 48
hr
 2 2858 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.80 NGFR rabbit IgG(H+L) / donkey NGFR 167.95
A375-B2-rep2
E7
COLO 858 Vemurafenib I-BET 0.3160 0.1067
uM
 48
hr
 2 2556 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 250.08 NGFR rabbit IgG(H+L) / donkey NGFR 76.21
Colo858-B2-rep2
E7
COLO 858 Vemurafenib I-BET 0.3160 0.5333
uM
 48
hr
 1 2681 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.14 NGFR rabbit IgG(H+L) / donkey NGFR 33.28
Colo858-B1-rep1
E7
A-375 Vemurafenib I-BET 1.0000 0.5333
uM
 48
hr
 1 2611 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 126.80 NGFR rabbit IgG(H+L) / donkey NGFR 177.94
A375-B1-rep1
E7