Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Garcinol 1.0000 2.6667
uM
 48
hr
 2 2002 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 190.44 NGFR rabbit IgG(H+L) / donkey NGFR 192.31
A375-A2-rep2
F3
WM-115 Vemurafenib Garcinol 1.0000 0.1067
uM
 48
hr
 1 1268 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.31 NGFR rabbit IgG(H+L) / donkey NGFR 1155.37
WM115-B2-rep1
F3
A-375 Vemurafenib Garcinol 1.0000 0.1067
uM
 48
hr
 1 2418 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 177.58 NGFR rabbit IgG(H+L) / donkey NGFR 200.14
A375-B2-rep1
F3
WM-115 Vemurafenib Garcinol 1.0000 0.5333
uM
 48
hr
 1 1246 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.16 NGFR rabbit IgG(H+L) / donkey NGFR 1098.79
WM115-B1-rep1
F3
WM-115 Vemurafenib Garcinol 1.0000 2.6667
uM
 48
hr
 2 936 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.05 NGFR rabbit IgG(H+L) / donkey NGFR 1030.71
WM115-A2-rep2
F3
A-375 Vemurafenib Garcinol 1.0000 2.6667
uM
 48
hr
 1 1325 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 146.88 NGFR rabbit IgG(H+L) / donkey NGFR 175.82
A375-A2-rep1
F3
A-375 Vemurafenib Garcinol 1.0000 0.5333
uM
 48
hr
 1 2101 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 167.05 NGFR rabbit IgG(H+L) / donkey NGFR 180.94
A375-B1-rep1
F3
COLO 858 Vemurafenib Garcinol 0.3160 0.1067
uM
 48
hr
 2 2520 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 363.75 NGFR rabbit IgG(H+L) / donkey NGFR 149.22
Colo858-B2-rep2
F3
WM-115 Vemurafenib Garcinol 1.0000 2.6667
uM
 48
hr
 1 758 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.60 NGFR rabbit IgG(H+L) / donkey NGFR 1025.88
WM115-A2-rep1
F3
WM-115 Vemurafenib GSK-J1 1.0000 0.1067
uM
 48
hr
 2 1158 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.88 NGFR rabbit IgG(H+L) / donkey NGFR 1182.56
WM115-B2-rep2
B6
A-375 Vemurafenib GSK-J1 1.0000 2.6667
uM
 48
hr
 1 2495 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 184.65 NGFR rabbit IgG(H+L) / donkey NGFR 225.50
A375-A2-rep1
B6
WM-115 Vemurafenib GSK-J1 1.0000 0.1067
uM
 48
hr
 1 1493 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.17 NGFR rabbit IgG(H+L) / donkey NGFR 1162.33
WM115-B2-rep1
B6
A-375 Vemurafenib GSK-J1 1.0000 0.1067
uM
 48
hr
 2 2557 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 175.06 NGFR rabbit IgG(H+L) / donkey NGFR 180.64
A375-B2-rep2
B6
COLO 858 Vemurafenib GSK-J1 0.3160 0.1067
uM
 48
hr
 2 1949 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 408.18 NGFR rabbit IgG(H+L) / donkey NGFR 166.40
Colo858-B2-rep2
B6
COLO 858 Vemurafenib GSK-J1 0.3160 0.5333
uM
 48
hr
 2 1597 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 332.46 NGFR rabbit IgG(H+L) / donkey NGFR 143.42
Colo858-B1-rep2
B6
WM-115 Vemurafenib GSK-J1 1.0000 2.6667
uM
 48
hr
 1 1200 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.71 NGFR rabbit IgG(H+L) / donkey NGFR 1023.37
WM115-A2-rep1
B6
COLO 858 Vemurafenib GSK-J1 0.3160 0.1067
uM
 48
hr
 1 1501 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 295.74 NGFR rabbit IgG(H+L) / donkey NGFR 156.47
Colo858-B2-rep1
B6
A-375 Vemurafenib GSK-J1 1.0000 0.5333
uM
 48
hr
 2 2749 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.27 NGFR rabbit IgG(H+L) / donkey NGFR 180.25
A375-B1-rep2
B6
WM-115 Vemurafenib GSK-J1 1.0000 0.5333
uM
 48
hr
 2 1323 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.46 NGFR rabbit IgG(H+L) / donkey NGFR 1138.62
WM115-B1-rep2
B6
COLO 858 Vemurafenib GSK-J1 0.3160 2.6667
uM
 48
hr
 1 1988 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 344.25 NGFR rabbit IgG(H+L) / donkey NGFR 140.14
Colo858-A2-rep1
B6
COLO 858 Vemurafenib GSK-J1 0.3160 0.5333
uM
 48
hr
 1 1919 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.19 NGFR rabbit IgG(H+L) / donkey NGFR 163.29
Colo858-B1-rep1
B6
WM-115 Vemurafenib GSK-J1 1.0000 0.5333
uM
 48
hr
 1 1268 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.27 NGFR rabbit IgG(H+L) / donkey NGFR 1155.24
WM115-B1-rep1
B6
COLO 858 Vemurafenib GSK-J1 0.3160 2.6667
uM
 48
hr
 2 1655 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.58 NGFR rabbit IgG(H+L) / donkey NGFR 139.46
Colo858-A2-rep2
B6
A-375 Vemurafenib GSK-J1 1.0000 2.6667
uM
 48
hr
 2 2734 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 198.78 NGFR rabbit IgG(H+L) / donkey NGFR 231.75
A375-A2-rep2
B6
A-375 Vemurafenib GSK-J1 1.0000 0.5333
uM
 48
hr
 1 2570 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 160.48 NGFR rabbit IgG(H+L) / donkey NGFR 156.00
A375-B1-rep1
B6