Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib C646 1.0000 2.6667
uM
 48
hr
 2 1111 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.86 NGFR rabbit IgG(H+L) / donkey NGFR 957.59
WM115-A2-rep2
E3
WM-115 Vemurafenib Mocetinostat 1.0000 2.6667
uM
 48
hr
 2 1114 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.59 NGFR rabbit IgG(H+L) / donkey NGFR 597.14
WM115-A2-rep2
E4
WM-115 Vemurafenib Resveratrol 1.0000 2.6667
uM
 48
hr
 2 1386 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.68 NGFR rabbit IgG(H+L) / donkey NGFR 1062.85
WM115-A2-rep2
E5
WM-115 Vemurafenib Daminozide 1.0000 2.6667
uM
 48
hr
 2 1444 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.62 NGFR rabbit IgG(H+L) / donkey NGFR 996.97
WM115-A2-rep2
E6
WM-115 Vemurafenib I-BET 1.0000 2.6667
uM
 48
hr
 2 1147 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 121.03 NGFR rabbit IgG(H+L) / donkey NGFR 490.84
WM115-A2-rep2
E7
WM-115 Vemurafenib Epigallocatechin gallate 1.0000 2.6667
uM
 48
hr
 2 1317 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 126.33 NGFR rabbit IgG(H+L) / donkey NGFR 1067.58
WM115-A2-rep2
E8
WM-115 Vemurafenib Decitabine 1.0000 2.6667
uM
 48
hr
 2 1225 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.80 NGFR rabbit IgG(H+L) / donkey NGFR 940.16
WM115-A2-rep2
F2
WM-115 Vemurafenib Garcinol 1.0000 2.6667
uM
 48
hr
 2 936 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.05 NGFR rabbit IgG(H+L) / donkey NGFR 1030.71
WM115-A2-rep2
F3
WM-115 Vemurafenib Pracinostat 1.0000 2.6667
uM
 48
hr
 2 775 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.68 NGFR rabbit IgG(H+L) / donkey NGFR 379.92
WM115-A2-rep2
F4
WM-115 Vemurafenib BIX-01294 1.0000 2.6667
uM
 48
hr
 2 865 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 241.06 NGFR rabbit IgG(H+L) / donkey NGFR 515.22
WM115-A2-rep2
F5
WM-115 Vemurafenib Methylstat 1.0000 2.6667
uM
 48
hr
 2 860 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 112.41 NGFR rabbit IgG(H+L) / donkey NGFR 846.07
WM115-A2-rep2
F6
WM-115 Vemurafenib I-BET151 1.0000 2.6667
uM
 48
hr
 2 768 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.34 NGFR rabbit IgG(H+L) / donkey NGFR 479.27
WM115-A2-rep2
F7
WM-115 Vemurafenib CX-5461 1.0000 2.6667
uM
 48
hr
 2 1216 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 116.78 NGFR rabbit IgG(H+L) / donkey NGFR 1052.21
WM115-A2-rep2
F8
WM-115 Vemurafenib RG-108 1.0000 2.6667
uM
 48
hr
 2 1106 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.86 NGFR rabbit IgG(H+L) / donkey NGFR 841.05
WM115-A2-rep2
G2
WM-115 Vemurafenib Anacardic acid 1.0000 2.6667
uM
 48
hr
 2 1013 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 132.50 NGFR rabbit IgG(H+L) / donkey NGFR 989.81
WM115-A2-rep2
G3
WM-115 Vemurafenib MC1568 1.0000 2.6667
uM
 48
hr
 2 1007 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 320.70 NGFR rabbit IgG(H+L) / donkey NGFR 977.00
WM115-A2-rep2
G4
WM-115 Vemurafenib UNC0638 1.0000 2.6667
uM
 48
hr
 2 1049 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 169.31 NGFR rabbit IgG(H+L) / donkey NGFR 874.75
WM115-A2-rep2
G5
WM-115 Vemurafenib Tranylcypromine 1.0000 2.6667
uM
 48
hr
 2 906 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.81 NGFR rabbit IgG(H+L) / donkey NGFR 1167.67
WM115-A2-rep2
G6
WM-115 Vemurafenib Ischemin 1.0000 2.6667
uM
 48
hr
 2 1033 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 128.78 NGFR rabbit IgG(H+L) / donkey NGFR 1025.85
WM115-A2-rep2
G7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 815 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.71 NGFR rabbit IgG(H+L) / donkey NGFR 826.75
WM115-A2-rep2
H10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 867 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.77 NGFR rabbit IgG(H+L) / donkey NGFR 905.23
WM115-A2-rep2
H11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 991 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.39 NGFR rabbit IgG(H+L) / donkey NGFR 912.83
WM115-A2-rep2
H2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 854 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.28 NGFR rabbit IgG(H+L) / donkey NGFR 899.54
WM115-A2-rep2
H3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 808 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.65 NGFR rabbit IgG(H+L) / donkey NGFR 855.37
WM115-A2-rep2
H4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 754 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.51 NGFR rabbit IgG(H+L) / donkey NGFR 910.39
WM115-A2-rep2
H5