Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1144 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 332.95 NGFR rabbit IgG(H+L) / donkey NGFR 125.32
Colo858-B2-rep1
A2
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2412 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 231.59 NGFR rabbit IgG(H+L) / donkey NGFR 226.51
A375-A2-rep2
A2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1314 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 162.02 NGFR rabbit IgG(H+L) / donkey NGFR 966.82
WM115-A2-rep1
A2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1304 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.81 NGFR rabbit IgG(H+L) / donkey NGFR 1013.49
WM115-B1-rep2
A2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1024 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.50 NGFR rabbit IgG(H+L) / donkey NGFR 135.78
Colo858-A2-rep1
A3
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2443 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 184.77 NGFR rabbit IgG(H+L) / donkey NGFR 168.94
A375-B2-rep1
A3
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2235 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.50 NGFR rabbit IgG(H+L) / donkey NGFR 181.65
A375-B1-rep1
A3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1434 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.89 NGFR rabbit IgG(H+L) / donkey NGFR 1166.16
WM115-B2-rep2
A3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1070 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 412.54 NGFR rabbit IgG(H+L) / donkey NGFR 139.52
Colo858-B2-rep2
A3
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2312 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.78 NGFR rabbit IgG(H+L) / donkey NGFR 179.41
A375-B1-rep2
A3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1319 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.08 NGFR rabbit IgG(H+L) / donkey NGFR 923.84
WM115-A2-rep2
A3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 915 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 295.91 NGFR rabbit IgG(H+L) / donkey NGFR 130.55
Colo858-B1-rep2
A3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 887 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 344.64 NGFR rabbit IgG(H+L) / donkey NGFR 133.90
Colo858-B1-rep1
A3
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2139 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 169.08 NGFR rabbit IgG(H+L) / donkey NGFR 191.16
A375-B2-rep2
A3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1253 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.31 NGFR rabbit IgG(H+L) / donkey NGFR 1049.31
WM115-B2-rep1
A3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1167 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 160.50 NGFR rabbit IgG(H+L) / donkey NGFR 1047.84
WM115-B1-rep2
A3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1054 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.53 NGFR rabbit IgG(H+L) / donkey NGFR 926.14
WM115-A2-rep1
A3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 928 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 297.12 NGFR rabbit IgG(H+L) / donkey NGFR 136.39
Colo858-B2-rep1
A3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 908 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.42 NGFR rabbit IgG(H+L) / donkey NGFR 130.36
Colo858-A2-rep2
A3
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2008 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 185.44 NGFR rabbit IgG(H+L) / donkey NGFR 205.73
A375-A2-rep1
A3
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2177 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 175.69 NGFR rabbit IgG(H+L) / donkey NGFR 218.26
A375-A2-rep2
A3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1254 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.90 NGFR rabbit IgG(H+L) / donkey NGFR 981.56
WM115-B1-rep1
A3
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2113 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 195.29 NGFR rabbit IgG(H+L) / donkey NGFR 185.68
A375-B2-rep2
A4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1202 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 368.53 NGFR rabbit IgG(H+L) / donkey NGFR 150.06
Colo858-B2-rep2
A4
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2171 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 208.08 NGFR rabbit IgG(H+L) / donkey NGFR 204.03
A375-A2-rep1
A4