Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1242 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 296.85 NGFR rabbit IgG(H+L) / donkey NGFR 156.51
Colo858-A2-rep1
A11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1440 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 346.44 NGFR rabbit IgG(H+L) / donkey NGFR 179.05
Colo858-B2-rep2
A11
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1793 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.73 NGFR rabbit IgG(H+L) / donkey NGFR 197.31
A375-A2-rep2
A11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1297 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.03 NGFR rabbit IgG(H+L) / donkey NGFR 965.78
WM115-A2-rep2
A11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1350 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 353.28 NGFR rabbit IgG(H+L) / donkey NGFR 136.44
Colo858-B1-rep1
A11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1405 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 151.46 NGFR rabbit IgG(H+L) / donkey NGFR 1114.77
WM115-B2-rep1
A11
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1490 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 206.53 NGFR rabbit IgG(H+L) / donkey NGFR 194.20
A375-B1-rep2
A11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1094 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 146.45 NGFR rabbit IgG(H+L) / donkey NGFR 1030.60
WM115-A2-rep1
A11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 994 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 353.13 NGFR rabbit IgG(H+L) / donkey NGFR 159.64
Colo858-B1-rep2
A11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1179 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.88 NGFR rabbit IgG(H+L) / donkey NGFR 1171.65
WM115-B2-rep2
A11
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1596 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 148.87 NGFR rabbit IgG(H+L) / donkey NGFR 151.79
A375-B2-rep2
A11
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2478 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.11 NGFR rabbit IgG(H+L) / donkey NGFR 175.65
A375-B2-rep1
A2
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2160 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 190.11 NGFR rabbit IgG(H+L) / donkey NGFR 151.59
A375-B1-rep1
A2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1304 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.81 NGFR rabbit IgG(H+L) / donkey NGFR 1013.49
WM115-B1-rep2
A2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1290 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.40 NGFR rabbit IgG(H+L) / donkey NGFR 1023.32
WM115-B1-rep1
A2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1358 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 340.31 NGFR rabbit IgG(H+L) / donkey NGFR 143.67
Colo858-B2-rep2
A2
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2412 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 231.59 NGFR rabbit IgG(H+L) / donkey NGFR 226.51
A375-A2-rep2
A2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1202 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 347.86 NGFR rabbit IgG(H+L) / donkey NGFR 141.29
Colo858-A2-rep1
A2
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2145 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 192.96 NGFR rabbit IgG(H+L) / donkey NGFR 200.10
A375-B2-rep2
A2
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2214 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 229.79 NGFR rabbit IgG(H+L) / donkey NGFR 205.29
A375-A2-rep1
A2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 906 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 347.23 NGFR rabbit IgG(H+L) / donkey NGFR 108.59
Colo858-B1-rep2
A2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1184 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 361.53 NGFR rabbit IgG(H+L) / donkey NGFR 140.40
Colo858-B1-rep1
A2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1033 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 359.83 NGFR rabbit IgG(H+L) / donkey NGFR 196.89
Colo858-A2-rep2
A2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1314 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 162.02 NGFR rabbit IgG(H+L) / donkey NGFR 966.82
WM115-A2-rep1
A2
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2108 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 195.39 NGFR rabbit IgG(H+L) / donkey NGFR 201.04
A375-B1-rep2
A2