Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Rocilinostat 1.0000 0.5333
uM
 48
hr
 1 1305 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.30 NGFR rabbit IgG(H+L) / donkey NGFR 965.10
WM115-B1-rep1
B5
A-375 Vemurafenib Rocilinostat 1.0000 0.1067
uM
 48
hr
 1 2966 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 230.12 NGFR rabbit IgG(H+L) / donkey NGFR 190.47
A375-B2-rep1
B5
A-375 Vemurafenib GSK-J1 1.0000 0.5333
uM
 48
hr
 1 2570 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 160.48 NGFR rabbit IgG(H+L) / donkey NGFR 156.00
A375-B1-rep1
B6
COLO 858 Vemurafenib GSK-J1 0.3160 0.5333
uM
 48
hr
 1 1919 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.19 NGFR rabbit IgG(H+L) / donkey NGFR 163.29
Colo858-B1-rep1
B6
WM-115 Vemurafenib GSK-J1 1.0000 2.6667
uM
 48
hr
 1 1200 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.71 NGFR rabbit IgG(H+L) / donkey NGFR 1023.37
WM115-A2-rep1
B6
WM-115 Vemurafenib GSK-J1 1.0000 0.5333
uM
 48
hr
 1 1268 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.27 NGFR rabbit IgG(H+L) / donkey NGFR 1155.24
WM115-B1-rep1
B6
COLO 858 Vemurafenib GSK-J1 0.3160 0.5333
uM
 48
hr
 2 1597 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 332.46 NGFR rabbit IgG(H+L) / donkey NGFR 143.42
Colo858-B1-rep2
B6
A-375 Vemurafenib GSK-J1 1.0000 0.1067
uM
 48
hr
 2 2557 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 175.06 NGFR rabbit IgG(H+L) / donkey NGFR 180.64
A375-B2-rep2
B6
COLO 858 Vemurafenib GSK-J1 0.3160 0.1067
uM
 48
hr
 2 1949 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 408.18 NGFR rabbit IgG(H+L) / donkey NGFR 166.40
Colo858-B2-rep2
B6
A-375 Vemurafenib GSK-J1 1.0000 0.5333
uM
 48
hr
 2 2749 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.27 NGFR rabbit IgG(H+L) / donkey NGFR 180.25
A375-B1-rep2
B6
COLO 858 Vemurafenib GSK-J1 0.3160 2.6667
uM
 48
hr
 1 1988 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 344.25 NGFR rabbit IgG(H+L) / donkey NGFR 140.14
Colo858-A2-rep1
B6
WM-115 Vemurafenib GSK-J1 1.0000 2.6667
uM
 48
hr
 2 1583 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.68 NGFR rabbit IgG(H+L) / donkey NGFR 1085.48
WM115-A2-rep2
B6
A-375 Vemurafenib GSK-J1 1.0000 2.6667
uM
 48
hr
 1 2495 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 184.65 NGFR rabbit IgG(H+L) / donkey NGFR 225.50
A375-A2-rep1
B6
A-375 Vemurafenib GSK-J1 1.0000 2.6667
uM
 48
hr
 2 2734 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 198.78 NGFR rabbit IgG(H+L) / donkey NGFR 231.75
A375-A2-rep2
B6
COLO 858 Vemurafenib GSK-J1 0.3160 0.1067
uM
 48
hr
 1 1501 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 295.74 NGFR rabbit IgG(H+L) / donkey NGFR 156.47
Colo858-B2-rep1
B6
WM-115 Vemurafenib GSK-J1 1.0000 0.1067
uM
 48
hr
 2 1158 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.88 NGFR rabbit IgG(H+L) / donkey NGFR 1182.56
WM115-B2-rep2
B6
COLO 858 Vemurafenib GSK-J1 0.3160 2.6667
uM
 48
hr
 2 1655 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.58 NGFR rabbit IgG(H+L) / donkey NGFR 139.46
Colo858-A2-rep2
B6
WM-115 Vemurafenib GSK-J1 1.0000 0.1067
uM
 48
hr
 1 1493 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.17 NGFR rabbit IgG(H+L) / donkey NGFR 1162.33
WM115-B2-rep1
B6
WM-115 Vemurafenib GSK-J1 1.0000 0.5333
uM
 48
hr
 2 1323 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.46 NGFR rabbit IgG(H+L) / donkey NGFR 1138.62
WM115-B1-rep2
B6
A-375 Vemurafenib GSK-J1 1.0000 0.1067
uM
 48
hr
 1 2690 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 178.62 NGFR rabbit IgG(H+L) / donkey NGFR 187.40
A375-B2-rep1
B6
A-375 Vemurafenib PFI-1 1.0000 0.5333
uM
 48
hr
 1 2521 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.70 NGFR rabbit IgG(H+L) / donkey NGFR 191.24
A375-B1-rep1
B7
COLO 858 Vemurafenib PFI-1 0.3160 0.1067
uM
 48
hr
 2 2090 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 400.42 NGFR rabbit IgG(H+L) / donkey NGFR 145.17
Colo858-B2-rep2
B7
A-375 Vemurafenib PFI-1 1.0000 0.1067
uM
 48
hr
 2 2198 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.45 NGFR rabbit IgG(H+L) / donkey NGFR 167.49
A375-B2-rep2
B7
COLO 858 Vemurafenib PFI-1 0.3160 0.5333
uM
 48
hr
 2 1454 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 310.50 NGFR rabbit IgG(H+L) / donkey NGFR 102.50
Colo858-B1-rep2
B7
WM-115 Vemurafenib PFI-1 1.0000 0.5333
uM
 48
hr
 1 1121 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 128.12 NGFR rabbit IgG(H+L) / donkey NGFR 999.98
WM115-B1-rep1
B7