Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1082 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 358.68 NGFR rabbit IgG(H+L) / donkey NGFR 117.87
Colo858-B1-rep1
A7
COLO 858 Vemurafenib GSK-J1 0.3160 0.5333
uM
 48
hr
 1 1919 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.19 NGFR rabbit IgG(H+L) / donkey NGFR 163.29
Colo858-B1-rep1
B6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1005 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 348.84 NGFR rabbit IgG(H+L) / donkey NGFR 143.10
Colo858-B1-rep1
A6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1008 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 371.10 NGFR rabbit IgG(H+L) / donkey NGFR 120.59
Colo858-B1-rep1
A5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1214 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 380.70 NGFR rabbit IgG(H+L) / donkey NGFR 139.87
Colo858-B1-rep1
A4
COLO 858 Vemurafenib Rucaparib 0.3160 0.5333
uM
 48
hr
 1 2060 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 271.45 NGFR rabbit IgG(H+L) / donkey NGFR 153.79
Colo858-B1-rep1
C3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 887 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 344.64 NGFR rabbit IgG(H+L) / donkey NGFR 133.90
Colo858-B1-rep1
A3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1184 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 361.53 NGFR rabbit IgG(H+L) / donkey NGFR 140.40
Colo858-B1-rep1
A2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1350 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 353.28 NGFR rabbit IgG(H+L) / donkey NGFR 136.44
Colo858-B1-rep1
A11
COLO 858 Vemurafenib (+)-JQ1 0.3160 0.5333
uM
 48
hr
 1 1371 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 102.15 NGFR rabbit IgG(H+L) / donkey NGFR 16.33
Colo858-B1-rep1
C7
COLO 858 Vemurafenib I-BET 0.3160 0.5333
uM
 48
hr
 1 2681 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.14 NGFR rabbit IgG(H+L) / donkey NGFR 33.28
Colo858-B1-rep1
E7
COLO 858 Vemurafenib Daminozide 0.3160 0.5333
uM
 48
hr
 1 3063 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 319.66 NGFR rabbit IgG(H+L) / donkey NGFR 144.83
Colo858-B1-rep1
E6
COLO 858 Vemurafenib Mocetinostat 0.3160 0.5333
uM
 48
hr
 1 2873 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.87 NGFR rabbit IgG(H+L) / donkey NGFR 44.51
Colo858-B1-rep1
E4
COLO 858 Vemurafenib C646 0.3160 0.5333
uM
 48
hr
 1 2531 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 280.51 NGFR rabbit IgG(H+L) / donkey NGFR 145.18
Colo858-B1-rep1
E3
COLO 858 Vemurafenib Azacitidine 0.3160 0.5333
uM
 48
hr
 1 1775 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 290.70 NGFR rabbit IgG(H+L) / donkey NGFR 98.06
Colo858-B1-rep1
E2
COLO 858 Vemurafenib IOX2 0.3160 0.5333
uM
 48
hr
 1 2770 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 335.63 NGFR rabbit IgG(H+L) / donkey NGFR 134.52
Colo858-B1-rep1
D8
COLO 858 Vemurafenib (-)-JQ1 0.3160 0.5333
uM
 48
hr
 1 3001 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 327.88 NGFR rabbit IgG(H+L) / donkey NGFR 161.24
Colo858-B1-rep1
D7
COLO 858 Vemurafenib GSK-J4 0.3160 0.5333
uM
 48
hr
 1 2928 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 284.13 NGFR rabbit IgG(H+L) / donkey NGFR 124.77
Colo858-B1-rep1
D6
COLO 858 Vemurafenib AGK2 0.3160 0.5333
uM
 48
hr
 1 1897 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 317.36 NGFR rabbit IgG(H+L) / donkey NGFR 134.31
Colo858-B1-rep1
D5
COLO 858 Vemurafenib Entinostat 0.3160 0.5333
uM
 48
hr
 1 2835 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 224.61 NGFR rabbit IgG(H+L) / donkey NGFR 79.92
Colo858-B1-rep1
D4
COLO 858 Vemurafenib JW55 0.3160 0.5333
uM
 48
hr
 1 2560 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 320.04 NGFR rabbit IgG(H+L) / donkey NGFR 134.51
Colo858-B1-rep1
D3
COLO 858 Vemurafenib Resveratrol 0.3160 0.5333
uM
 48
hr
 1 2695 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 316.17 NGFR rabbit IgG(H+L) / donkey NGFR 134.95
Colo858-B1-rep1
E5
COLO 858 Vemurafenib Vorinostat 0.3160 0.5333
uM
 48
hr
 1 1313 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 213.34 NGFR rabbit IgG(H+L) / donkey NGFR 151.95
Colo858-B1-rep1
D2
COLO 858 Vemurafenib UNC1215 0.3160 0.5333
uM
 48
hr
 1 2463 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 322.32 NGFR rabbit IgG(H+L) / donkey NGFR 160.13
Colo858-B1-rep1
C8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1121 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 328.09 NGFR rabbit IgG(H+L) / donkey NGFR 131.17
Colo858-B1-rep1
A10