Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 610 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 248.64 NGFR rabbit IgG(H+L) / donkey NGFR 141.66
Colo858-B1-rep2
H3
COLO 858 Vemurafenib AGK2 0.3160 2.6667
uM
 48
hr
 2 635 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 266.45 NGFR rabbit IgG(H+L) / donkey NGFR 71.47
Colo858-A2-rep2
D5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 673 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 276.26 NGFR rabbit IgG(H+L) / donkey NGFR 130.08
Colo858-A2-rep1
H5
COLO 858 Vemurafenib Azacitidine 0.3160 2.6667
uM
 48
hr
 2 674 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 228.99 NGFR rabbit IgG(H+L) / donkey NGFR 27.17
Colo858-A2-rep2
E2
A-375 Vemurafenib AGK2 1.0000 2.6667
uM
 48
hr
 1 678 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 394.51 NGFR rabbit IgG(H+L) / donkey NGFR 132.96
A375-A2-rep1
D5
COLO 858 Vemurafenib GSK-J4 0.3160 2.6667
uM
 48
hr
 2 682 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 189.00 NGFR rabbit IgG(H+L) / donkey NGFR 11.65
Colo858-A2-rep2
D6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 683 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 277.61 NGFR rabbit IgG(H+L) / donkey NGFR 110.87
Colo858-A2-rep1
H4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 685 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 333.97 NGFR rabbit IgG(H+L) / donkey NGFR 138.64
Colo858-B1-rep2
A8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 685 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 282.57 NGFR rabbit IgG(H+L) / donkey NGFR 147.29
Colo858-B1-rep2
H5
WM-115 Vemurafenib Belinostat 1.0000 2.6667
uM
 48
hr
 2 690 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.25 NGFR rabbit IgG(H+L) / donkey NGFR 284.20
WM115-A2-rep2
C4
WM-115 Vemurafenib I-BET151 1.0000 2.6667
uM
 48
hr
 1 691 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.59 NGFR rabbit IgG(H+L) / donkey NGFR 497.01
WM115-A2-rep1
F7
WM-115 Vemurafenib Belinostat 1.0000 2.6667
uM
 48
hr
 1 705 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.59 NGFR rabbit IgG(H+L) / donkey NGFR 342.51
WM115-A2-rep1
C4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 709 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 278.92 NGFR rabbit IgG(H+L) / donkey NGFR 116.26
Colo858-B2-rep1
H3
COLO 858 Vemurafenib BIX-01294 0.3160 2.6667
uM
 48
hr
 2 721 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.48 NGFR rabbit IgG(H+L) / donkey NGFR 30.74
Colo858-A2-rep2
F5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 728 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 275.60 NGFR rabbit IgG(H+L) / donkey NGFR 129.02
Colo858-A2-rep2
H4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 746 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.54 NGFR rabbit IgG(H+L) / donkey NGFR 1007.03
WM115-B2-rep1
H5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 747 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.67 NGFR rabbit IgG(H+L) / donkey NGFR 1024.66
WM115-B1-rep2
H7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 748 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 229.27 NGFR rabbit IgG(H+L) / donkey NGFR 160.07
Colo858-B2-rep1
H4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 754 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.51 NGFR rabbit IgG(H+L) / donkey NGFR 910.39
WM115-A2-rep2
H5
COLO 858 Vemurafenib Rocilinostat 0.3160 2.6667
uM
 48
hr
 1 755 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 163.81 NGFR rabbit IgG(H+L) / donkey NGFR 184.22
Colo858-A2-rep1
B5
WM-115 Vemurafenib Garcinol 1.0000 2.6667
uM
 48
hr
 1 758 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.60 NGFR rabbit IgG(H+L) / donkey NGFR 1025.88
WM115-A2-rep1
F3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 764 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 266.14 NGFR rabbit IgG(H+L) / donkey NGFR 145.66
Colo858-B1-rep2
H6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 764 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 263.12 NGFR rabbit IgG(H+L) / donkey NGFR 148.13
Colo858-B1-rep2
H8
WM-115 Vemurafenib I-BET151 1.0000 2.6667
uM
 48
hr
 2 768 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.34 NGFR rabbit IgG(H+L) / donkey NGFR 479.27
WM115-A2-rep2
F7
WM-115 Vemurafenib Pracinostat 1.0000 2.6667
uM
 48
hr
 2 775 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.68 NGFR rabbit IgG(H+L) / donkey NGFR 379.92
WM115-A2-rep2
F4