Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1106 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 325.17 NGFR rabbit IgG(H+L) / donkey NGFR 144.51
Colo858-B1-rep2
A4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1108 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 147.71 NGFR rabbit IgG(H+L) / donkey NGFR 912.06
WM115-A2-rep1
H10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1108 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 148.07 NGFR rabbit IgG(H+L) / donkey NGFR 1162.21
WM115-B2-rep2
H2
WM-115 Vemurafenib C646 1.0000 2.6667
uM
 48
hr
 2 1111 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.86 NGFR rabbit IgG(H+L) / donkey NGFR 957.59
WM115-A2-rep2
E3
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1111 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.94 NGFR rabbit IgG(H+L) / donkey NGFR 127.79
A375-B1-rep1
H8
WM-115 Vemurafenib UNC0638 1.0000 0.1067
uM
 48
hr
 1 1111 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.67 NGFR rabbit IgG(H+L) / donkey NGFR 1220.72
WM115-B2-rep1
G5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1112 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.20 NGFR rabbit IgG(H+L) / donkey NGFR 1115.20
WM115-B1-rep1
A6
WM-115 Vemurafenib Mocetinostat 1.0000 2.6667
uM
 48
hr
 2 1114 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.59 NGFR rabbit IgG(H+L) / donkey NGFR 597.14
WM115-A2-rep2
E4
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1114 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 185.61 NGFR rabbit IgG(H+L) / donkey NGFR 154.43
A375-B1-rep2
H6
WM-115 Vemurafenib Garcinol 1.0000 0.5333
uM
 48
hr
 2 1114 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.06 NGFR rabbit IgG(H+L) / donkey NGFR 1016.89
WM115-B1-rep2
F3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1117 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 282.92 NGFR rabbit IgG(H+L) / donkey NGFR 133.48
Colo858-B2-rep2
H4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1117 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 406.39 NGFR rabbit IgG(H+L) / donkey NGFR 145.48
Colo858-A2-rep2
A5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1118 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.18 NGFR rabbit IgG(H+L) / donkey NGFR 1039.92
WM115-B2-rep2
H9
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1118 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.53 NGFR rabbit IgG(H+L) / donkey NGFR 1078.75
WM115-B2-rep2
H4
WM-115 Vemurafenib PFI-1 1.0000 0.5333
uM
 48
hr
 1 1121 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 128.12 NGFR rabbit IgG(H+L) / donkey NGFR 999.98
WM115-B1-rep1
B7
WM-115 Vemurafenib Ischemin 1.0000 2.6667
uM
 48
hr
 1 1121 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.01 NGFR rabbit IgG(H+L) / donkey NGFR 989.25
WM115-A2-rep1
G7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1121 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 328.09 NGFR rabbit IgG(H+L) / donkey NGFR 131.17
Colo858-B1-rep1
A10
WM-115 Vemurafenib UNC0638 1.0000 2.6667
uM
 48
hr
 1 1121 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 170.32 NGFR rabbit IgG(H+L) / donkey NGFR 760.80
WM115-A2-rep1
G5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1122 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 296.79 NGFR rabbit IgG(H+L) / donkey NGFR 157.80
Colo858-A2-rep1
A5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1122 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 307.07 NGFR rabbit IgG(H+L) / donkey NGFR 147.66
Colo858-B1-rep1
H7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1123 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.11 NGFR rabbit IgG(H+L) / donkey NGFR 1122.60
WM115-B2-rep2
A7
COLO 858 Vemurafenib MC1568 0.3160 2.6667
uM
 48
hr
 2 1124 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.62 NGFR rabbit IgG(H+L) / donkey NGFR 110.02
Colo858-A2-rep2
G4
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1126 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 214.28 NGFR rabbit IgG(H+L) / donkey NGFR 175.81
A375-B1-rep2
H5
WM-115 Vemurafenib C646 1.0000 0.5333
uM
 48
hr
 2 1128 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 128.60 NGFR rabbit IgG(H+L) / donkey NGFR 1048.49
WM115-B1-rep2
E3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1132 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 307.65 NGFR rabbit IgG(H+L) / donkey NGFR 141.85
Colo858-B1-rep1
H5