Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib (+)-JQ1 1.0000 0.5333
uM
 48
hr
 2 1082 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.57 NGFR rabbit IgG(H+L) / donkey NGFR 548.46
WM115-B1-rep2
C7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1082 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.35 NGFR rabbit IgG(H+L) / donkey NGFR 1063.55
WM115-B1-rep1
A11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1082 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 358.68 NGFR rabbit IgG(H+L) / donkey NGFR 117.87
Colo858-B1-rep1
A7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1086 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.77 NGFR rabbit IgG(H+L) / donkey NGFR 1029.13
WM115-B1-rep1
H9
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1087 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.27 NGFR rabbit IgG(H+L) / donkey NGFR 138.42
A375-A2-rep1
H9
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1088 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.47 NGFR rabbit IgG(H+L) / donkey NGFR 155.22
A375-A2-rep1
H10
WM-115 Vemurafenib CX-5461 1.0000 2.6667
uM
 48
hr
 1 1088 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.79 NGFR rabbit IgG(H+L) / donkey NGFR 1051.81
WM115-A2-rep1
F8
WM-115 Vemurafenib RG-108 1.0000 2.6667
uM
 48
hr
 1 1089 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.86 NGFR rabbit IgG(H+L) / donkey NGFR 782.30
WM115-A2-rep1
G2
WM-115 Vemurafenib Ischemin 1.0000 0.1067
uM
 48
hr
 1 1092 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.85 NGFR rabbit IgG(H+L) / donkey NGFR 1107.34
WM115-B2-rep1
G7
WM-115 Vemurafenib Anacardic acid 1.0000 0.1067
uM
 48
hr
 1 1092 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.00 NGFR rabbit IgG(H+L) / donkey NGFR 967.15
WM115-B2-rep1
G3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1092 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.32 NGFR rabbit IgG(H+L) / donkey NGFR 1076.20
WM115-B2-rep2
H6
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1092 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.52 NGFR rabbit IgG(H+L) / donkey NGFR 1059.09
WM115-B1-rep1
A5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1093 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.58 NGFR rabbit IgG(H+L) / donkey NGFR 1088.13
WM115-A2-rep1
A10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1094 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 146.45 NGFR rabbit IgG(H+L) / donkey NGFR 1030.60
WM115-A2-rep1
A11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1094 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.83 NGFR rabbit IgG(H+L) / donkey NGFR 922.23
WM115-A2-rep1
H6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1095 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 337.39 NGFR rabbit IgG(H+L) / donkey NGFR 164.33
Colo858-A2-rep1
A7
WM-115 Vemurafenib UNC0638 1.0000 0.5333
uM
 48
hr
 1 1095 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 146.03 NGFR rabbit IgG(H+L) / donkey NGFR 1097.91
WM115-B1-rep1
G5
COLO 858 Vemurafenib MC1568 0.3160 0.5333
uM
 48
hr
 2 1098 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 323.03 NGFR rabbit IgG(H+L) / donkey NGFR 124.26
Colo858-B1-rep2
G4
WM-115 Vemurafenib Anacardic acid 1.0000 0.5333
uM
 48
hr
 1 1099 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.32 NGFR rabbit IgG(H+L) / donkey NGFR 1027.42
WM115-B1-rep1
G3
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1099 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.81 NGFR rabbit IgG(H+L) / donkey NGFR 136.50
A375-A2-rep1
H8
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1100 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.13 NGFR rabbit IgG(H+L) / donkey NGFR 1179.05
WM115-B2-rep2
A10
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1100 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 184.49 NGFR rabbit IgG(H+L) / donkey NGFR 149.00
A375-B1-rep2
H7
WM-115 Vemurafenib RG-108 1.0000 0.5333
uM
 48
hr
 2 1105 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.17 NGFR rabbit IgG(H+L) / donkey NGFR 1056.85
WM115-B1-rep2
G2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1105 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 336.45 NGFR rabbit IgG(H+L) / donkey NGFR 169.68
Colo858-B2-rep1
A9
WM-115 Vemurafenib RG-108 1.0000 2.6667
uM
 48
hr
 2 1106 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.86 NGFR rabbit IgG(H+L) / donkey NGFR 841.05
WM115-A2-rep2
G2