Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Mocetinostat 0.3160 0.5333
uM
 48
hr
 1 2873 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.87 NGFR rabbit IgG(H+L) / donkey NGFR 44.51
Colo858-B1-rep1
E4
A-375 Vemurafenib Pracinostat 1.0000 0.5333
uM
 48
hr
 1 1766 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 425.92 NGFR rabbit IgG(H+L) / donkey NGFR 51.29
A375-B1-rep1
F4
A-375 Vemurafenib Azacitidine 1.0000 2.6667
uM
 48
hr
 1 1210 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 261.75 NGFR rabbit IgG(H+L) / donkey NGFR 53.65
A375-A2-rep1
E2
A-375 Vemurafenib Methylstat 1.0000 2.6667
uM
 48
hr
 1 1079 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.72 NGFR rabbit IgG(H+L) / donkey NGFR 55.48
A375-A2-rep1
F6
A-375 Vemurafenib UNC0638 1.0000 2.6667
uM
 48
hr
 1 1266 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 181.34 NGFR rabbit IgG(H+L) / donkey NGFR 57.07
A375-A2-rep1
G5
A-375 Vemurafenib Vorinostat 1.0000 2.6667
uM
 48
hr
 1 1890 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 229.09 NGFR rabbit IgG(H+L) / donkey NGFR 60.09
A375-A2-rep1
D2
A-375 Vemurafenib Pracinostat 1.0000 0.5333
uM
 48
hr
 2 2095 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 492.96 NGFR rabbit IgG(H+L) / donkey NGFR 61.23
A375-B1-rep2
F4
A-375 Vemurafenib Vorinostat 1.0000 2.6667
uM
 48
hr
 2 2222 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 252.58 NGFR rabbit IgG(H+L) / donkey NGFR 61.42
A375-A2-rep2
D2
A-375 Vemurafenib Azacitidine 1.0000 2.6667
uM
 48
hr
 2 1303 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 286.33 NGFR rabbit IgG(H+L) / donkey NGFR 62.73
A375-A2-rep2
E2
COLO 858 Vemurafenib Belinostat 0.3160 0.1067
uM
 48
hr
 1 798 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 187.52 NGFR rabbit IgG(H+L) / donkey NGFR 66.34
Colo858-B2-rep1
C4
COLO 858 Vemurafenib Methylstat 0.3160 2.6667
uM
 48
hr
 1 1783 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 213.42 NGFR rabbit IgG(H+L) / donkey NGFR 68.18
Colo858-A2-rep1
F6
A-375 Vemurafenib I-BET151 1.0000 2.6667
uM
 48
hr
 1 1612 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.73 NGFR rabbit IgG(H+L) / donkey NGFR 68.89
A375-A2-rep1
F7
COLO 858 Vemurafenib Methylstat 0.3160 2.6667
uM
 48
hr
 2 1461 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.86 NGFR rabbit IgG(H+L) / donkey NGFR 69.08
Colo858-A2-rep2
F6
COLO 858 Vemurafenib AGK2 0.3160 2.6667
uM
 48
hr
 2 635 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 266.45 NGFR rabbit IgG(H+L) / donkey NGFR 71.47
Colo858-A2-rep2
D5
A-375 Vemurafenib I-BET151 1.0000 2.6667
uM
 48
hr
 2 1718 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.96 NGFR rabbit IgG(H+L) / donkey NGFR 72.08
A375-A2-rep2
F7
A-375 Vemurafenib UNC0638 1.0000 2.6667
uM
 48
hr
 2 1362 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 190.15 NGFR rabbit IgG(H+L) / donkey NGFR 72.59
A375-A2-rep2
G5
A-375 Vemurafenib (+)-JQ1 1.0000 0.5333
uM
 48
hr
 2 2283 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.15 NGFR rabbit IgG(H+L) / donkey NGFR 74.90
A375-B1-rep2
C7
COLO 858 Vemurafenib I-BET 0.3160 0.1067
uM
 48
hr
 1 2640 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 228.39 NGFR rabbit IgG(H+L) / donkey NGFR 75.66
Colo858-B2-rep1
E7
COLO 858 Vemurafenib I-BET 0.3160 0.1067
uM
 48
hr
 2 2556 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 250.08 NGFR rabbit IgG(H+L) / donkey NGFR 76.21
Colo858-B2-rep2
E7
A-375 Vemurafenib Rocilinostat 1.0000 2.6667
uM
 48
hr
 2 2940 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 277.54 NGFR rabbit IgG(H+L) / donkey NGFR 76.23
A375-A2-rep2
B5
A-375 Vemurafenib (+)-JQ1 1.0000 0.5333
uM
 48
hr
 1 2279 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.52 NGFR rabbit IgG(H+L) / donkey NGFR 79.90
A375-B1-rep1
C7
COLO 858 Vemurafenib Entinostat 0.3160 0.5333
uM
 48
hr
 1 2835 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 224.61 NGFR rabbit IgG(H+L) / donkey NGFR 79.92
Colo858-B1-rep1
D4
A-375 Vemurafenib (+)-JQ1 1.0000 2.6667
uM
 48
hr
 1 1957 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.74 NGFR rabbit IgG(H+L) / donkey NGFR 79.92
A375-A2-rep1
C7
COLO 858 Vemurafenib Entinostat 0.3160 0.5333
uM
 48
hr
 2 2701 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 211.88 NGFR rabbit IgG(H+L) / donkey NGFR 80.70
Colo858-B1-rep2
D4
A-375 Vemurafenib Mocetinostat 1.0000 2.6667
uM
 48
hr
 2 533 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 254.36 NGFR rabbit IgG(H+L) / donkey NGFR 81.09
A375-A2-rep2
E4