Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib (+)-JQ1 0.3160 0.1067
uM
 48
hr
 1 1564 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 110.51 NGFR rabbit IgG(H+L) / donkey NGFR 23.82
Colo858-B2-rep1
C7
COLO 858 Vemurafenib I-BET 0.3160 0.5333
uM
 48
hr
 2 2224 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 161.45 NGFR rabbit IgG(H+L) / donkey NGFR 24.73
Colo858-B1-rep2
E7
COLO 858 Vemurafenib BIX-01294 0.3160 2.6667
uM
 48
hr
 1 561 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 189.27 NGFR rabbit IgG(H+L) / donkey NGFR 24.74
Colo858-A2-rep1
F5
COLO 858 Vemurafenib Pracinostat 0.3160 0.5333
uM
 48
hr
 2 326 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 166.82 NGFR rabbit IgG(H+L) / donkey NGFR 26.66
Colo858-B1-rep2
F4
A-375 Vemurafenib Belinostat 1.0000 2.6667
uM
 48
hr
 2 319 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 360.58 NGFR rabbit IgG(H+L) / donkey NGFR 26.94
A375-A2-rep2
C4
COLO 858 Vemurafenib Azacitidine 0.3160 2.6667
uM
 48
hr
 2 674 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 228.99 NGFR rabbit IgG(H+L) / donkey NGFR 27.17
Colo858-A2-rep2
E2
COLO 858 Vemurafenib (+)-JQ1 0.3160 0.1067
uM
 48
hr
 2 2000 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.50 NGFR rabbit IgG(H+L) / donkey NGFR 27.98
Colo858-B2-rep2
C7
COLO 858 Vemurafenib Vorinostat 0.3160 2.6667
uM
 48
hr
 2 478 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 242.50 NGFR rabbit IgG(H+L) / donkey NGFR 28.47
Colo858-A2-rep2
D2
COLO 858 Vemurafenib Pracinostat 0.3160 2.6667
uM
 48
hr
 1 169 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 222.43 NGFR rabbit IgG(H+L) / donkey NGFR 28.52
Colo858-A2-rep1
F4
COLO 858 Vemurafenib Belinostat 0.3160 0.5333
uM
 48
hr
 1 394 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 228.28 NGFR rabbit IgG(H+L) / donkey NGFR 28.69
Colo858-B1-rep1
C4
COLO 858 Vemurafenib PFI-1 0.3160 2.6667
uM
 48
hr
 1 1383 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.34 NGFR rabbit IgG(H+L) / donkey NGFR 30.41
Colo858-A2-rep1
B7
COLO 858 Vemurafenib BIX-01294 0.3160 2.6667
uM
 48
hr
 2 721 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.48 NGFR rabbit IgG(H+L) / donkey NGFR 30.74
Colo858-A2-rep2
F5
COLO 858 Vemurafenib I-BET151 0.3160 0.1067
uM
 48
hr
 1 1645 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 181.50 NGFR rabbit IgG(H+L) / donkey NGFR 33.08
Colo858-B2-rep1
F7
A-375 Vemurafenib BIX-01294 1.0000 2.6667
uM
 48
hr
 1 825 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 295.68 NGFR rabbit IgG(H+L) / donkey NGFR 33.17
A375-A2-rep1
F5
COLO 858 Vemurafenib I-BET 0.3160 0.5333
uM
 48
hr
 1 2681 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.14 NGFR rabbit IgG(H+L) / donkey NGFR 33.28
Colo858-B1-rep1
E7
COLO 858 Vemurafenib Pracinostat 0.3160 0.5333
uM
 48
hr
 1 456 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 184.16 NGFR rabbit IgG(H+L) / donkey NGFR 34.51
Colo858-B1-rep1
F4
A-375 Vemurafenib Pracinostat 1.0000 2.6667
uM
 48
hr
 2 215 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 234.28 NGFR rabbit IgG(H+L) / donkey NGFR 35.01
A375-A2-rep2
F4
A-375 Vemurafenib Belinostat 1.0000 0.1067
uM
 48
hr
 1 2949 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 194.46 NGFR rabbit IgG(H+L) / donkey NGFR 37.06
A375-B2-rep1
C4
COLO 858 Vemurafenib Pracinostat 0.3160 2.6667
uM
 48
hr
 2 127 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 200.69 NGFR rabbit IgG(H+L) / donkey NGFR 37.13
Colo858-A2-rep2
F4
A-375 Vemurafenib Pracinostat 1.0000 2.6667
uM
 48
hr
 1 137 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 388.80 NGFR rabbit IgG(H+L) / donkey NGFR 39.73
A375-A2-rep1
F4
COLO 858 Vemurafenib Mocetinostat 0.3160 0.5333
uM
 48
hr
 2 2847 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.05 NGFR rabbit IgG(H+L) / donkey NGFR 41.69
Colo858-B1-rep2
E4
COLO 858 Vemurafenib I-BET151 0.3160 0.1067
uM
 48
hr
 2 2368 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 194.16 NGFR rabbit IgG(H+L) / donkey NGFR 42.29
Colo858-B2-rep2
F7
A-375 Vemurafenib BIX-01294 1.0000 2.6667
uM
 48
hr
 2 1250 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 292.19 NGFR rabbit IgG(H+L) / donkey NGFR 42.51
A375-A2-rep2
F5
A-375 Vemurafenib Methylstat 1.0000 2.6667
uM
 48
hr
 2 1381 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.28 NGFR rabbit IgG(H+L) / donkey NGFR 43.97
A375-A2-rep2
F6
A-375 Vemurafenib Belinostat 1.0000 0.1067
uM
 48
hr
 2 2898 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 184.66 NGFR rabbit IgG(H+L) / donkey NGFR 44.08
A375-B2-rep2
C4