Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 821 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 254.01 NGFR rabbit IgG(H+L) / donkey NGFR 137.66
Colo858-B2-rep1
H5
COLO 858 Vemurafenib Azacitidine 0.3160 0.1067
uM
 48
hr
 2 2234 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 274.09 NGFR rabbit IgG(H+L) / donkey NGFR 137.68
Colo858-B2-rep2
E2
COLO 858 Vemurafenib AGK2 0.3160 0.1067
uM
 48
hr
 2 2858 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 305.97 NGFR rabbit IgG(H+L) / donkey NGFR 137.70
Colo858-B2-rep2
D5
A-375 Vemurafenib RG-108 1.0000 0.1067
uM
 48
hr
 1 2074 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.70 NGFR rabbit IgG(H+L) / donkey NGFR 138.05
A375-B2-rep1
G2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 903 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 227.64 NGFR rabbit IgG(H+L) / donkey NGFR 138.37
Colo858-A2-rep2
H7
COLO 858 Vemurafenib Pracinostat 0.3160 0.1067
uM
 48
hr
 2 1001 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.71 NGFR rabbit IgG(H+L) / donkey NGFR 138.38
Colo858-B2-rep2
F4
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1087 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.27 NGFR rabbit IgG(H+L) / donkey NGFR 138.42
A375-A2-rep1
H9
A-375 Vemurafenib Tranylcypromine 1.0000 0.1067
uM
 48
hr
 2 1897 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.61 NGFR rabbit IgG(H+L) / donkey NGFR 138.48
A375-B2-rep2
G6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 685 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 333.97 NGFR rabbit IgG(H+L) / donkey NGFR 138.64
Colo858-B1-rep2
A8
A-375 Vemurafenib JW55 1.0000 2.6667
uM
 48
hr
 1 2571 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.73 NGFR rabbit IgG(H+L) / donkey NGFR 138.78
A375-A2-rep1
D3
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1364 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 201.35 NGFR rabbit IgG(H+L) / donkey NGFR 138.83
A375-B1-rep2
H11
A-375 Vemurafenib I-BET 1.0000 0.5333
uM
 48
hr
 2 2710 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.52 NGFR rabbit IgG(H+L) / donkey NGFR 139.02
A375-B1-rep2
E7
COLO 858 Vemurafenib BIX-01294 0.3160 0.1067
uM
 48
hr
 2 2596 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 356.20 NGFR rabbit IgG(H+L) / donkey NGFR 139.03
Colo858-B2-rep2
F5
A-375 Vemurafenib RG-108 1.0000 0.1067
uM
 48
hr
 2 2074 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.49 NGFR rabbit IgG(H+L) / donkey NGFR 139.25
A375-B2-rep2
G2
COLO 858 Vemurafenib Resveratrol 0.3160 0.5333
uM
 48
hr
 2 2772 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 301.42 NGFR rabbit IgG(H+L) / donkey NGFR 139.46
Colo858-B1-rep2
E5
COLO 858 Vemurafenib GSK-J1 0.3160 2.6667
uM
 48
hr
 2 1655 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.58 NGFR rabbit IgG(H+L) / donkey NGFR 139.46
Colo858-A2-rep2
B6
A-375 Vemurafenib CTB 1.0000 0.5333
uM
 48
hr
 2 2694 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.97 NGFR rabbit IgG(H+L) / donkey NGFR 139.51
A375-B1-rep2
B4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1070 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 412.54 NGFR rabbit IgG(H+L) / donkey NGFR 139.52
Colo858-B2-rep2
A3
A-375 Vemurafenib RG-108 1.0000 2.6667
uM
 48
hr
 2 1857 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 209.06 NGFR rabbit IgG(H+L) / donkey NGFR 139.68
A375-A2-rep2
G2
COLO 858 Vemurafenib Daminozide 0.3160 0.5333
uM
 48
hr
 2 3157 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 329.58 NGFR rabbit IgG(H+L) / donkey NGFR 139.83
Colo858-B1-rep2
E6
COLO 858 Vemurafenib C646 0.3160 0.1067
uM
 48
hr
 2 2646 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 378.38 NGFR rabbit IgG(H+L) / donkey NGFR 139.85
Colo858-B2-rep2
E3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1214 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 380.70 NGFR rabbit IgG(H+L) / donkey NGFR 139.87
Colo858-B1-rep1
A4
COLO 858 Vemurafenib Garcinol 0.3160 2.6667
uM
 48
hr
 2 1133 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 279.50 NGFR rabbit IgG(H+L) / donkey NGFR 140.01
Colo858-A2-rep2
F3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 964 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 357.17 NGFR rabbit IgG(H+L) / donkey NGFR 140.07
Colo858-B2-rep2
A7
COLO 858 Vemurafenib Ischemin 0.3160 0.5333
uM
 48
hr
 2 1246 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 336.98 NGFR rabbit IgG(H+L) / donkey NGFR 140.12
Colo858-B1-rep2
G7