Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

 Full screen view
Filter results: >>>>
Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib (+)-JQ1 0.3160 2.6667
uM
 48
hr
 1 1745 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.39 NGFR rabbit IgG(H+L) / donkey NGFR 10.46
Colo858-A2-rep1
C7
WM-115 Vemurafenib AGK2 1.0000 0.1067
uM
 48
hr
 2 1468 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.46 NGFR rabbit IgG(H+L) / donkey NGFR 1223.13
WM115-B2-rep2
D5
WM-115 Vemurafenib Methylstat 1.0000 0.1067
uM
 48
hr
 1 1231 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.55 NGFR rabbit IgG(H+L) / donkey NGFR 1100.39
WM115-B2-rep1
F6
WM-115 Vemurafenib (+)-JQ1 1.0000 0.5333
uM
 48
hr
 2 1082 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.57 NGFR rabbit IgG(H+L) / donkey NGFR 548.46
WM115-B1-rep2
C7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 867 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.77 NGFR rabbit IgG(H+L) / donkey NGFR 905.23
WM115-A2-rep2
H11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1002 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.83 NGFR rabbit IgG(H+L) / donkey NGFR 994.93
WM115-B2-rep2
H11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1040 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.93 NGFR rabbit IgG(H+L) / donkey NGFR 791.91
WM115-A2-rep1
H8
WM-115 Vemurafenib Mocetinostat 1.0000 0.1067
uM
 48
hr
 1 1586 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.99 NGFR rabbit IgG(H+L) / donkey NGFR 1412.07
WM115-B2-rep1
E4
WM-115 Vemurafenib CX-5461 1.0000 0.1067
uM
 48
hr
 1 1311 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.99 NGFR rabbit IgG(H+L) / donkey NGFR 1203.80
WM115-B2-rep1
F8
WM-115 Vemurafenib IOX2 1.0000 0.5333
uM
 48
hr
 2 1361 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.11 NGFR rabbit IgG(H+L) / donkey NGFR 1071.77
WM115-B1-rep2
D8
WM-115 Vemurafenib CX-5461 1.0000 0.1067
uM
 48
hr
 2 1332 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.12 NGFR rabbit IgG(H+L) / donkey NGFR 1219.65
WM115-B2-rep2
F8
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 821 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.47 NGFR rabbit IgG(H+L) / donkey NGFR 902.73
WM115-B1-rep2
H6
WM-115 Vemurafenib Epigallocatechin gallate 1.0000 2.6667
uM
 48
hr
 1 1303 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.63 NGFR rabbit IgG(H+L) / donkey NGFR 1092.09
WM115-A2-rep1
E8
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 815 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.71 NGFR rabbit IgG(H+L) / donkey NGFR 826.75
WM115-A2-rep2
H10
A-375 Vemurafenib I-BET151 1.0000 2.6667
uM
 48
hr
 1 1612 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.73 NGFR rabbit IgG(H+L) / donkey NGFR 68.89
A375-A2-rep1
F7
A-375 Vemurafenib (+)-JQ1 1.0000 2.6667
uM
 48
hr
 1 1957 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.74 NGFR rabbit IgG(H+L) / donkey NGFR 79.92
A375-A2-rep1
C7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 904 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.03 NGFR rabbit IgG(H+L) / donkey NGFR 939.97
WM115-B2-rep1
H6
WM-115 Vemurafenib I-BET151 1.0000 0.5333
uM
 48
hr
 2 1142 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.08 NGFR rabbit IgG(H+L) / donkey NGFR 686.83
WM115-B1-rep2
F7
WM-115 Vemurafenib Methylstat 1.0000 0.1067
uM
 48
hr
 2 1428 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.16 NGFR rabbit IgG(H+L) / donkey NGFR 1139.78
WM115-B2-rep2
F6
WM-115 Vemurafenib Entinostat 1.0000 0.5333
uM
 48
hr
 1 1649 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.31 NGFR rabbit IgG(H+L) / donkey NGFR 1011.83
WM115-B1-rep1
D4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1092 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.32 NGFR rabbit IgG(H+L) / donkey NGFR 1076.20
WM115-B2-rep2
H6
WM-115 Vemurafenib I-BET151 1.0000 0.5333
uM
 48
hr
 1 1213 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.34 NGFR rabbit IgG(H+L) / donkey NGFR 683.96
WM115-B1-rep1
F7
WM-115 Vemurafenib Epigallocatechin gallate 1.0000 0.5333
uM
 48
hr
 2 1274 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.39 NGFR rabbit IgG(H+L) / donkey NGFR 1161.43
WM115-B1-rep2
E8
WM-115 Vemurafenib Tranylcypromine 1.0000 0.1067
uM
 48
hr
 1 1145 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.44 NGFR rabbit IgG(H+L) / donkey NGFR 997.49
WM115-B2-rep1
G6
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 892 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.44 NGFR rabbit IgG(H+L) / donkey NGFR 1032.39
WM115-B1-rep2
H10