Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 996 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.05 NGFR rabbit IgG(H+L) / donkey NGFR 1083.30
WM115-A2-rep1
A9
A-375 Vemurafenib Rucaparib 1.0000 2.6667
uM
 48
hr
 1 2038 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.07 NGFR rabbit IgG(H+L) / donkey NGFR 172.41
A375-A2-rep1
C3
WM-115 Vemurafenib Resveratrol 1.0000 0.1067
uM
 48
hr
 2 1381 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.09 NGFR rabbit IgG(H+L) / donkey NGFR 1087.55
WM115-B2-rep2
E5
WM-115 Vemurafenib AGK2 1.0000 2.6667
uM
 48
hr
 1 193 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.10 NGFR rabbit IgG(H+L) / donkey NGFR 876.79
WM115-A2-rep1
D5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1123 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.11 NGFR rabbit IgG(H+L) / donkey NGFR 1122.60
WM115-B2-rep2
A7
COLO 858 Vemurafenib Entinostat 0.3160 2.6667
uM
 48
hr
 2 2000 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.13 NGFR rabbit IgG(H+L) / donkey NGFR 12.84
Colo858-A2-rep2
D4
WM-115 Vemurafenib JW55 1.0000 0.1067
uM
 48
hr
 2 1482 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.32 NGFR rabbit IgG(H+L) / donkey NGFR 1155.40
WM115-B2-rep2
D3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1136 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.45 NGFR rabbit IgG(H+L) / donkey NGFR 946.39
WM115-B1-rep1
H10
WM-115 Vemurafenib Resveratrol 1.0000 0.5333
uM
 48
hr
 2 1298 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.55 NGFR rabbit IgG(H+L) / donkey NGFR 1087.58
WM115-B1-rep2
E5
WM-115 Vemurafenib Selisistat 1.0000 0.1067
uM
 48
hr
 2 1468 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.56 NGFR rabbit IgG(H+L) / donkey NGFR 1089.03
WM115-B2-rep2
C5
WM-115 Vemurafenib Rucaparib 1.0000 0.1067
uM
 48
hr
 1 1484 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.57 NGFR rabbit IgG(H+L) / donkey NGFR 1094.92
WM115-B2-rep1
C3
WM-115 Vemurafenib Resveratrol 1.0000 0.1067
uM
 48
hr
 1 1377 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.59 NGFR rabbit IgG(H+L) / donkey NGFR 1187.81
WM115-B2-rep1
E5
WM-115 Vemurafenib Decitabine 1.0000 2.6667
uM
 48
hr
 1 1175 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.60 NGFR rabbit IgG(H+L) / donkey NGFR 898.23
WM115-A2-rep1
F2
A-375 Vemurafenib I-BET151 1.0000 0.1067
uM
 48
hr
 2 2822 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.62 NGFR rabbit IgG(H+L) / donkey NGFR 182.94
A375-B2-rep2
F7
WM-115 Vemurafenib GSK-J1 1.0000 2.6667
uM
 48
hr
 1 1200 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.71 NGFR rabbit IgG(H+L) / donkey NGFR 1023.37
WM115-A2-rep1
B6
WM-115 Vemurafenib Olaparib 1.0000 2.6667
uM
 48
hr
 2 1137 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.72 NGFR rabbit IgG(H+L) / donkey NGFR 1028.30
WM115-A2-rep2
B2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1150 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.75 NGFR rabbit IgG(H+L) / donkey NGFR 1097.89
WM115-B2-rep2
H10
WM-115 Vemurafenib Ischemin 1.0000 0.1067
uM
 48
hr
 1 1092 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.85 NGFR rabbit IgG(H+L) / donkey NGFR 1107.34
WM115-B2-rep1
G7
WM-115 Vemurafenib Decitabine 1.0000 0.1067
uM
 48
hr
 2 1393 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.04 NGFR rabbit IgG(H+L) / donkey NGFR 1121.73
WM115-B2-rep2
F2
WM-115 Vemurafenib (+)-JQ1 1.0000 0.1067
uM
 48
hr
 1 1197 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.08 NGFR rabbit IgG(H+L) / donkey NGFR 701.57
WM115-B2-rep1
C7
WM-115 Vemurafenib Garcinol 1.0000 0.1067
uM
 48
hr
 2 1397 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.18 NGFR rabbit IgG(H+L) / donkey NGFR 1141.90
WM115-B2-rep2
F3
WM-115 Vemurafenib Belinostat 1.0000 2.6667
uM
 48
hr
 2 690 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.25 NGFR rabbit IgG(H+L) / donkey NGFR 284.20
WM115-A2-rep2
C4
WM-115 Vemurafenib IOX2 1.0000 0.1067
uM
 48
hr
 1 1508 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.26 NGFR rabbit IgG(H+L) / donkey NGFR 1073.48
WM115-B2-rep1
D8
WM-115 Vemurafenib GSK-J4 1.0000 0.1067
uM
 48
hr
 1 1589 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.26 NGFR rabbit IgG(H+L) / donkey NGFR 1214.14
WM115-B2-rep1
D6
WM-115 Vemurafenib UNC669 1.0000 0.5333
uM
 48
hr
 2 1455 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.47 NGFR rabbit IgG(H+L) / donkey NGFR 1276.18
WM115-B1-rep2
B8