Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib UNC669 1.0000 0.1067
uM
 48
hr
 2 2020 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.07 NGFR rabbit IgG(H+L) / donkey NGFR 160.38
A375-B2-rep2
B8
WM-115 Vemurafenib UNC1215 1.0000 0.1067
uM
 48
hr
 1 1541 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.07 NGFR rabbit IgG(H+L) / donkey NGFR 1112.68
WM115-B2-rep1
C8
WM-115 Vemurafenib (-)-JQ1 1.0000 0.1067
uM
 48
hr
 2 1607 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.27 NGFR rabbit IgG(H+L) / donkey NGFR 1139.85
WM115-B2-rep2
D7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1253 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.31 NGFR rabbit IgG(H+L) / donkey NGFR 1049.31
WM115-B2-rep1
A3
WM-115 Vemurafenib Iniparib 1.0000 0.5333
uM
 48
hr
 2 1282 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.32 NGFR rabbit IgG(H+L) / donkey NGFR 1147.41
WM115-B1-rep2
B3
WM-115 Vemurafenib Anacardic acid 1.0000 0.5333
uM
 48
hr
 1 1099 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.32 NGFR rabbit IgG(H+L) / donkey NGFR 1027.42
WM115-B1-rep1
G3
COLO 858 Vemurafenib PFI-1 0.3160 2.6667
uM
 48
hr
 1 1383 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.34 NGFR rabbit IgG(H+L) / donkey NGFR 30.41
Colo858-A2-rep1
B7
WM-115 Vemurafenib JW55 1.0000 0.5333
uM
 48
hr
 2 1270 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.41 NGFR rabbit IgG(H+L) / donkey NGFR 1093.58
WM115-B1-rep2
D3
WM-115 Vemurafenib Rocilinostat 1.0000 0.1067
uM
 48
hr
 2 1382 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.42 NGFR rabbit IgG(H+L) / donkey NGFR 1083.28
WM115-B2-rep2
B5
WM-115 Vemurafenib Vorinostat 1.0000 2.6667
uM
 48
hr
 1 1431 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.45 NGFR rabbit IgG(H+L) / donkey NGFR 436.29
WM115-A2-rep1
D2
WM-115 Vemurafenib Tranylcypromine 1.0000 0.5333
uM
 48
hr
 2 900 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.52 NGFR rabbit IgG(H+L) / donkey NGFR 1071.63
WM115-B1-rep2
G6
WM-115 Vemurafenib UNC669 1.0000 0.1067
uM
 48
hr
 2 1142 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.65 NGFR rabbit IgG(H+L) / donkey NGFR 1180.01
WM115-B2-rep2
B8
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1086 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.77 NGFR rabbit IgG(H+L) / donkey NGFR 1029.13
WM115-B1-rep1
H9
A-375 Vemurafenib (+)-JQ1 1.0000 0.1067
uM
 48
hr
 1 2815 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.87 NGFR rabbit IgG(H+L) / donkey NGFR 142.58
A375-B2-rep1
C7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 971 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.87 NGFR rabbit IgG(H+L) / donkey NGFR 1060.23
WM115-B1-rep1
H8
WM-115 Vemurafenib Vorinostat 1.0000 0.1067
uM
 48
hr
 2 1513 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.96 NGFR rabbit IgG(H+L) / donkey NGFR 1082.99
WM115-B2-rep2
D2
WM-115 Vemurafenib PFI-1 1.0000 0.1067
uM
 48
hr
 1 1493 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.99 NGFR rabbit IgG(H+L) / donkey NGFR 1089.78
WM115-B2-rep1
B7
WM-115 Vemurafenib Iniparib 1.0000 2.6667
uM
 48
hr
 1 1294 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.06 NGFR rabbit IgG(H+L) / donkey NGFR 1014.90
WM115-A2-rep1
B3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 930 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.08 NGFR rabbit IgG(H+L) / donkey NGFR 1077.20
WM115-A2-rep1
A7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 952 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.09 NGFR rabbit IgG(H+L) / donkey NGFR 915.79
WM115-B2-rep1
H11
WM-115 Vemurafenib CTB 1.0000 2.6667
uM
 48
hr
 2 1474 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.13 NGFR rabbit IgG(H+L) / donkey NGFR 836.64
WM115-A2-rep2
B4
WM-115 Vemurafenib Garcinol 1.0000 0.5333
uM
 48
hr
 1 1246 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.16 NGFR rabbit IgG(H+L) / donkey NGFR 1098.79
WM115-B1-rep1
F3
WM-115 Vemurafenib GSK-J1 1.0000 0.5333
uM
 48
hr
 2 1323 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.46 NGFR rabbit IgG(H+L) / donkey NGFR 1138.62
WM115-B1-rep2
B6
WM-115 Vemurafenib AGK2 1.0000 0.1067
uM
 48
hr
 1 1420 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.59 NGFR rabbit IgG(H+L) / donkey NGFR 1264.14
WM115-B2-rep1
D5
WM-115 Vemurafenib Veliparib 1.0000 0.1067
uM
 48
hr
 2 1355 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.84 NGFR rabbit IgG(H+L) / donkey NGFR 1032.08
WM115-B2-rep2
C2