Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Tranylcypromine 1.0000 0.1067
uM
 48
hr
 1 2043 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.99 NGFR rabbit IgG(H+L) / donkey NGFR 192.09
A375-B2-rep1
G6
A-375 Vemurafenib Ischemin 1.0000 0.1067
uM
 48
hr
 1 1998 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 186.72 NGFR rabbit IgG(H+L) / donkey NGFR 161.90
A375-B2-rep1
G7
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1702 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.33 NGFR rabbit IgG(H+L) / donkey NGFR 152.80
A375-B2-rep1
H10
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1693 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.82 NGFR rabbit IgG(H+L) / donkey NGFR 130.50
A375-B2-rep1
H11
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1685 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.63 NGFR rabbit IgG(H+L) / donkey NGFR 141.16
A375-B2-rep1
H2
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1675 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.32 NGFR rabbit IgG(H+L) / donkey NGFR 154.64
A375-B2-rep1
H3
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1748 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 166.30 NGFR rabbit IgG(H+L) / donkey NGFR 148.34
A375-B2-rep1
H4
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1713 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 183.53 NGFR rabbit IgG(H+L) / donkey NGFR 151.47
A375-B2-rep1
H5
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1586 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.41 NGFR rabbit IgG(H+L) / donkey NGFR 151.52
A375-B2-rep1
H6
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1496 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.86 NGFR rabbit IgG(H+L) / donkey NGFR 154.03
A375-B2-rep1
H7
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1588 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.67 NGFR rabbit IgG(H+L) / donkey NGFR 144.38
A375-B2-rep1
H8
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1733 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 161.19 NGFR rabbit IgG(H+L) / donkey NGFR 146.36
A375-B2-rep1
H9
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1910 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.46 NGFR rabbit IgG(H+L) / donkey NGFR 175.04
A375-B2-rep2
A10
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1596 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 148.87 NGFR rabbit IgG(H+L) / donkey NGFR 151.79
A375-B2-rep2
A11
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2145 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 192.96 NGFR rabbit IgG(H+L) / donkey NGFR 200.10
A375-B2-rep2
A2
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2139 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 169.08 NGFR rabbit IgG(H+L) / donkey NGFR 191.16
A375-B2-rep2
A3
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2113 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 195.29 NGFR rabbit IgG(H+L) / donkey NGFR 185.68
A375-B2-rep2
A4
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1882 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 161.60 NGFR rabbit IgG(H+L) / donkey NGFR 180.56
A375-B2-rep2
A5
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1875 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 176.75 NGFR rabbit IgG(H+L) / donkey NGFR 195.09
A375-B2-rep2
A6
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1569 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.54 NGFR rabbit IgG(H+L) / donkey NGFR 186.02
A375-B2-rep2
A7
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1668 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.45 NGFR rabbit IgG(H+L) / donkey NGFR 154.12
A375-B2-rep2
A8
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2082 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 165.98 NGFR rabbit IgG(H+L) / donkey NGFR 171.03
A375-B2-rep2
A9
A-375 Vemurafenib Olaparib 1.0000 0.1067
uM
 48
hr
 2 2141 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.10 NGFR rabbit IgG(H+L) / donkey NGFR 172.84
A375-B2-rep2
B2
A-375 Vemurafenib Iniparib 1.0000 0.1067
uM
 48
hr
 2 2469 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.14 NGFR rabbit IgG(H+L) / donkey NGFR 203.97
A375-B2-rep2
B3
A-375 Vemurafenib CTB 1.0000 0.1067
uM
 48
hr
 2 2227 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 169.07 NGFR rabbit IgG(H+L) / donkey NGFR 150.81
A375-B2-rep2
B4